Search Results - "Debaene, François"

Innovative Native MS Methodologies for Antibody Drug Conjugate Characterization: High Resolution Native MS and IM-MS for Average DAR and DAR Distribution Assessment by Debaene, François, Bœuf, Amandine, Wagner-Rousset, Elsa, Colas, Olivier, Ayoub, Daniel, Corvaïa, Nathalie, Van Dorsselaer, Alain, Beck, Alain, Cianférani, Sarah

“…Antibody drug conjugates (ADCs) are macromolecules composed of cytotoxic drugs covalently attached via a conditionally stable linker to monoclonal antibodies…”
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Cutting-edge mass spectrometry characterization of originator, biosimilar and biobetter antibodies by Beck, Alain, Debaene, François, Diemer, Hélène, Wagner-Rousset, Elsa, Colas, Olivier, Dorsselaer, Alain Van, Cianférani, Sarah

“…The approval process for antibody biosimilars relies primarily on comprehensive analytical data to establish comparability and high similarity with the…”
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CBTF: New Amine-to-Thiol Coupling Reagent for Preparation of Antibody Conjugates with Increased Plasma Stability by Kolodych, Sergii, Koniev, Oleksandr, Baatarkhuu, Zoljargal, Bonnefoy, Jean-Yves, Debaene, François, Cianférani, Sarah, Van Dorsselaer, Alain, Wagner, Alain

“…Amine-to-thiol coupling is the most common route for the preparation of antibody–drug conjugates (ADC). It is usually achieved by using heterobifunctional…”
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Time Resolved Native Ion-Mobility Mass Spectrometry to Monitor Dynamics of IgG4 Fab Arm Exchange and “Bispecific” Monoclonal Antibody Formation by Debaene, François, Wagner-Rousset, Elsa, Colas, Olivier, Ayoub, Daniel, Corvaïa, Nathalie, Van Dorsselaer, Alain, Beck, Alain, Cianférani, Sarah

“…Monoclonal antibodies (mAbs) and derivatives such as antibody–drug conjugates (ADC) and bispecific antibodies (bsAb), are the fastest growing class of human…”
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Analytical characterization of biosimilar antibodies and Fc-fusion proteins by Beck, Alain, Diemer, Hélène, Ayoub, Daniel, Debaene, François, Wagner-Rousset, Elsa, Carapito, Christine, Van Dorsselaer, Alain, Sanglier-Cianférani, Sarah

“…•Extensive biosimilar/reference mAb comparison is required in biosimilar development.•In EU and USA, biosimilar/reference mAb primary amino-acid sequences must…”
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Supramolecular Luminescent Lanthanide Dimers for Fluoride Sequestering and Sensing by Liu, Tao, Nonat, Aline, Beyler, Maryline, Regueiro-Figueroa, Martín, Nchimi Nono, Katia, Jeannin, Olivier, Camerel, Franck, Debaene, François, Cianférani-Sanglier, Sarah, Tripier, Raphaël, Platas-Iglesias, Carlos, Charbonnière, Loïc J.

“…Lanthanide complexes (Ln=Eu, Tb, and Yb) that are based on a C2‐symmetric cyclen scaffold were prepared and characterized. The addition of fluoride anions to…”
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Cutting-edge mass spectrometry methods for the multi-level structural characterization of antibody-drug conjugates by Beck, Alain, Terral, Guillaume, Debaene, François, Wagner-Rousset, Elsa, Marcoux, Julien, Janin-Bussat, Marie-Claire, Colas, Olivier, Van Dorsselaer, Alain, Cianférani, Sarah

“…Antibody drug conjugates (ADCs) are highly cytotoxic drugs covalently attached via conditionally stable linkers to monoclonal antibodies (mAbs) and are among…”
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Screening approaches for the identification of Nrf2-Keap1 protein-protein interaction inhibitors targeting hot spot residues by Asano, Wataru, Hantani, Rie, Uhara, Toru, Debaene, François, Nomura, Akihiro, Yamaguchi, Keishi, Adachi, Tsuyoshi, Otake, Kazuki, Harada, Kazuhito, Hantani, Yoshiji

“…Protein-protein interactions (PPIs) play a crucial role in most biological processes and are important targets in the development of therapeutic agents…”
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Epitope characterization of anti-JAM-A antibodies using orthogonal mass spectrometry and surface plasmon resonance approaches by Terral, Guillaume, Champion, Thierry, Debaene, François, Colas, Olivier, Bourguet, Maxime, Wagner-Rousset, Elsa, Corvaia, Nathalie, Beck, Alain, Cianferani, Sarah

“…Junctional adhesion molecule-A (JAM-A) is an adherens and tight junction protein expressed by endothelial and epithelial cells and associated with cancer…”
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Launching Spiking Ligands into a Protein–Protein Interface: A Promising Strategy To Destabilize and Break Interface Formation in a tRNA Modifying Enzyme by Immekus, Florian, Barandun, Luzi Jakob, Betz, Michael, Debaene, François, Petiot, Stéphanie, Sanglier-Cianferani, Sarah, Reuter, Klaus, Diederich, François, Klebe, Gerhard

“…Apart from competitive active-site inhibition of protein function, perturbance of protein–protein interactions by small molecules in oligodomain enzymes opens…”
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Hot-spot analysis to dissect the functional protein-protein interface of a tRNA-modifying enzyme by Jakobi, Stephan, Nguyen, Tran Xuan Phong, Debaene, François, Metz, Alexander, Sanglier-Cianférani, Sarah, Reuter, Klaus, Klebe, Gerhard

“…ABSTRACT Interference with protein–protein interactions of interfaces larger than 1500 Å2 by small drug‐like molecules is notoriously difficult, particularly…”
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Expanding the scope of PNA-encoded libraries: divergent synthesis of libraries targeting cysteine, serine and metallo-proteases as well as tyrosine phosphatases by Debaene, François, Da Silva, Julien A., Pianowski, Zbigniew, Duran, Fernando J., Winssinger, Nicolas

“…Seven PNA-encoded combinatorial libraries targeting proteases and phosphatases with covalent reversible and irreversible mechanism-based inhibitors were…”
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Soaking suggests "alternative facts": Only co-crystallization discloses major ligand-induced interface rearrangements of a homodimeric tRNA-binding protein indicating a novel mode-of-inhibition by Ehrmann, Frederik Rainer, Stojko, Johann, Metz, Alexander, Debaene, François, Barandun, Luzi Jakob, Heine, Andreas, Diederich, François, Cianférani, Sarah, Reuter, Klaus, Klebe, Gerhard

“…For the efficient pathogenesis of Shigella, the causative agent of bacillary dysentery, full functionality of tRNA-guanine transglycosylase (TGT) is mandatory…”
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A phenyl-thiadiazolylidene-amine derivative ejects zinc from retroviral nucleocapsid zinc fingers and inactivates HIV virions by Vercruysse, Thomas, Basta, Beata, Dehaen, Wim, Humbert, Nicolas, Balzarini, Jan, Debaene, François, Sanglier-Cianférani, Sarah, Pannecouque, Christophe, Mély, Yves, Daelemans, Dirk

“…Sexual acquisition of the human immunodeficiency virus (HIV) through mucosal transmission may be prevented by using topically applied agents that block HIV…”
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Self-Assembled Small-Molecule Microarrays for Protease Screening and Profiling by Urbina, Hugo D, Debaene, François, Jost, Bernard, Bole-Feysot, Christine, Mason, Daniel E, Kuzmic, Petr, Harris, Jennifer L, Winssinger, Nicolas

“…Small-molecule microarrays are attractive for chemical biology as they permit the analysis of hundreds to thousands of interactions in a highly miniaturized…”
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Synthesis of a PNA-encoded cysteine protease inhibitor library by Debaene, François, Mejias, Lorenzo, Harris, Jennifer L., Winssinger, Nicolas

“…Peptide nucleic acids (PNAs) have been used to encode a combinatorial library whereby each compound is labeled with a PNA tag which reflects its synthetic…”
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Simultaneous Self-Assembly of a [2]Catenane, a Trefoil Knot, and a Solomon Link from a Simple Pair of Ligands by Prakasam, Thirumurugan, Lusi, Matteo, Elhabiri, Mourad, Platas-Iglesias, Carlos, Olsen, John-Carl, Asfari, Zouhair, Cianférani-Sanglier, Sarah, Debaene, François, Charbonnière, Loïc J., Trabolsi, Ali

“…A topological triptych: Three molecular links, a [2]catenane, a trefoil knot, and a Solomon link, were obtained in one pot through the self‐assembly of two…”
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The Importance of Charge in Perturbing the Aromatic Glue Stabilizing the Protein–Protein Interface of Homodimeric tRNA-Guanine Transglycosylase by Nguyen, Andreas, Nguyen, Dzung, Phong Nguyen, Tran Xuan, Sebastiani, Maurice, Dörr, Stefanie, Hernandez-Alba, Oscar, Debaene, François, Cianférani, Sarah, Heine, Andreas, Klebe, Gerhard, Reuter, Klaus

“…Bacterial tRNA-guanine transglycosylase (Tgt) is involved in the biosynthesis of the modified tRNA nucleoside queuosine present in the anticodon wobble…”
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Supramolecular Luminescent Lanthanide Dimers for Fluoride Sequestering and Sensing by Liu, Tao, Nonat, Aline, Beyler, Maryline, Regueiro-Figueroa, Martín, Nchimi Nono, Katia, Jeannin, Olivier, Camerel, Franck, Debaene, François, Cianférani-Sanglier, Sarah, Tripier, Raphaël, Platas-Iglesias, Carlos, Charbonnière, Loïc J.

“…Lanthanide complexes (Ln=Eu, Tb, and Yb) that are based on a C2‐symmetric cyclen scaffold were prepared and characterized. The addition of fluoride anions to…”
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Structural and functional studies of ReP1-NCXSQ, a protein regulating the squid nerve Na+/Ca2+ exchanger by Cousido-Siah, Alexandra, Ayoub, Daniel, Berberián, Graciela, Bollo, Mariana, Van Dorsselaer, Alain, Debaene, François, DiPolo, Reinaldo, Petrova, Tatiana, Schulze-Briese, Clemens, Olieric, Vincent, Esteves, Adriana, Mitschler, André, Sanglier-Cianférani, Sarah, Beaugé, Luis, Podjarny, Alberto

“…The protein ReP1‐NCXSQ was isolated from the cytosol of squid nerves and has been shown to be required for MgATP stimulation of the squid nerve Na+/Ca2+…”
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