Avian lungs: A novel scaffold for lung bioengineering

Allogeneic lung transplant is limited both by the shortage of available donor lungs and by the lack of suitable long-term lung assist devices to bridge patients to lung transplantation. Avian lungs have different structure and mechanics resulting in more efficient gas exchange than mammalian lungs....

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Bibliographic Details
Published in:	PloS one Vol. 13; no. 6; p. e0198956
Main Authors:	Wrenn, Sean M, Griswold, Ethan D, Uhl, Franziska E, Uriarte, Juan J, Park, Heon E, Coffey, Amy L, Dearborn, Jacob S, Ahlers, Bethany A, Deng, Bin, Lam, Ying-Wai, Huston, Dryver R, Lee, Patrick C, Wagner, Darcy E, Weiss, Daniel J
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 27-06-2018 Public Library of Science (PLoS)
Subjects:	Anaphylaxis Animal models Animals Apoptosis Bioengineering Bioengineering - methods Biological research Biology Biology and Life Sciences Birds Cardiac and Cardiovascular Systems Cell adhesion Cell Proliferation Chickens Clinical Medicine Deoxyribonucleic acid DNA DNA structure Dromaiidae Dromaius novaehollandiae Electron microscopy Endothelial cells Epithelial cells Extracellular matrix Extracellular Matrix - metabolism Farms Feasibility studies Fibroblasts Gallus gallus domesticus Gas exchange Heart Humans Kardiologi Klinisk medicin Lung - cytology Lung diseases Lung transplantation Lungs Mammals Mechanical engineering Medical and Health Sciences Medicin och hälsovetenskap Medicine Medicine and Health Sciences Mesenchyme Ostomy Poultry Proteins Proteomics Pulmonary arteries Quantitation Research and Analysis Methods Respiratory diseases Respiratory tract Scaffolds Spectrometry Stem cells Stromal cells Throat Tissue Scaffolds Transplantation Transplants & implants United States > US
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Summary:	Allogeneic lung transplant is limited both by the shortage of available donor lungs and by the lack of suitable long-term lung assist devices to bridge patients to lung transplantation. Avian lungs have different structure and mechanics resulting in more efficient gas exchange than mammalian lungs. Decellularized avian lungs, recellularized with human lung cells, could therefore provide a powerful novel gas exchange unit for potential use in pulmonary therapeutics. To initially assess this in both small and large avian lung models, chicken (Gallus gallus domesticus) and emu (Dromaius novaehollandiae) lungs were decellularized using modifications of a detergent-based protocol, previously utilized with mammalian lungs. Light and electron microscopy, vascular and airway resistance, quantitation and gel analyses of residual DNA, and immunohistochemical and mass spectrometric analyses of remaining extracellular matrix (ECM) proteins demonstrated maintenance of lung structure, minimal residual DNA, and retention of major ECM proteins in the decellularized scaffolds. Seeding with human bronchial epithelial cells, human pulmonary vascular endothelial cells, human mesenchymal stromal cells, and human lung fibroblasts demonstrated initial cell attachment on decellularized avian lungs and growth over a 7-day period. These initial studies demonstrate that decellularized avian lungs may be a feasible approach for generating functional lung tissue for clinical therapeutics.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0198956