Search Results - "DeWitt, Mark A"

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA by Richardson, Christopher D, Ray, Graham J, DeWitt, Mark A, Curie, Gemma L, Corn, Jacob E

“…The efficiency of homology-directed genome editing with CRISPR-Cas9 is boosted through improved design of donor DNA. Targeted genomic manipulation by Cas9 can…”
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Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells by DeWitt, Mark A, Magis, Wendy, Bray, Nicolas L, Wang, Tianjiao, Berman, Jennifer R, Urbinati, Fabrizia, Heo, Seok-Jin, Mitros, Therese, Muñoz, Denise P, Boffelli, Dario, Kohn, Donald B, Walters, Mark C, Carroll, Dana, Martin, David I K, Corn, Jacob E

“…Genetic diseases of blood cells are prime candidates for treatment through ex vivo gene editing of CD34 hematopoietic stem/progenitor cells (HSPCs), and a…”
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Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering by Lee, Kunwoo, Mackley, Vanessa A, Rao, Anirudh, Chong, Anthony T, Dewitt, Mark A, Corn, Jacob E, Murthy, Niren

“…Chemical modification of the gRNA and donor DNA has great potential for improving the gene editing efficiency of Cas9 and Cpf1, but has not been investigated…”
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Cytoplasmic Dynein Moves Through Uncoordinated Stepping of the AAA+ Ring Domains by DeWitt, Mark A., Chang, Amy Y., Combs, Peter A., Yildiz, Ahmet

“…Cytoplasmic dynein is a homodimenc AAA+ motor that transports a multitude of cargos toward the microtubule minus end. How the two catalytic head domains…”
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Improving Gene Editing Outcomes in Human Hematopoietic Stem and Progenitor Cells by Temporal Control of DNA Repair by Lomova, Anastasia, Clark, Danielle N., Campo‐Fernandez, Beatriz, Flores‐Bjurström, Carmen, Kaufman, Michael L., Fitz‐Gibbon, Sorel, Wang, Xiaoyan, Miyahira, Eric Y., Brown, Devin, DeWitt, Mark A., Corn, Jacob E., Hollis, Roger P., Romero, Zulema, Kohn, Donald B.

“…Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated system (Cas9)‐mediated gene editing of human hematopoietic stem cells…”
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Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair by Lee, Kunwoo, Conboy, Michael, Park, Hyo Min, Jiang, Fuguo, Kim, Hyun Jin, Dewitt, Mark A., Mackley, Vanessa A., Chang, Kevin, Rao, Anirudh, Skinner, Colin, Shobha, Tamanna, Mehdipour, Melod, Liu, Hui, Huang, Wen-chin, Lan, Freeman, Bray, Nicolas L., Li, Song, Corn, Jacob E., Kataoka, Kazunori, Doudna, Jennifer A., Conboy, Irina, Murthy, Niren

“…Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR associated protein 9 (Cas9)-based therapeutics, especially those that can correct…”
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The AAA3 domain of cytoplasmic dynein acts as a switch to facilitate microtubule release by DeWitt, Mark A, Cypranowska, Caroline A, Cleary, Frank B, Belyy, Vladislav, Yildiz, Ahmet

“…Cytoplasmic dynein has multiple ATPase subunits, with AAA1 as the primary ATPase. Single-molecule and biochemical approaches reveal that AAA3 ATPase has a role…”
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Distance Mapping in Proteins Using Fluorescence Spectroscopy: The Tryptophan-Induced Quenching (TrIQ) Method by Mansoor, Steven E, DeWitt, Mark A, Farrens, David L

“…Studying the interplay between protein structure and function remains a daunting task. Especially lacking are methods for measuring structural changes in real…”
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Tension on the linker gates the ATP-dependent release of dynein from microtubules by Cleary, Frank B., Dewitt, Mark A., Bilyard, Thomas, Htet, Zaw Min, Belyy, Vladislav, Chan, Danna D., Chang, Amy Y., Yildiz, Ahmet

“…Cytoplasmic dynein is a dimeric motor that transports intracellular cargoes towards the minus end of microtubules (MTs). In contrast to other processive…”
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Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin by Ravi, Nithin Sam, Wienert, Beeke, Wyman, Stacia K, Bell, Henry William, George, Anila, Mahalingam, Gokulnath, Vu, Jonathan T, Prasad, Kirti, Bandlamudi, Bhanu Prasad, Devaraju, Nivedhitha, Rajendiran, Vignesh, Syedbasha, Nazar, Pai, Aswin Anand, Nakamura, Yukio, Kurita, Ryo, Narayanasamy, Muthuraman, Balasubramanian, Poonkuzhali, Thangavel, Saravanabhavan, Marepally, Srujan, Velayudhan, Shaji R, Srivastava, Alok, DeWitt, Mark A, Crossley, Merlin, Corn, Jacob E, Mohankumar, Kumarasamypet M

“…Naturally occurring point mutations in the promoter switch hemoglobin synthesis from defective adult beta-globin to fetal gamma-globin in sickle cell patients…”
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Bidirectional helical motility of cytoplasmic dynein around microtubules by Can, Sinan, Dewitt, Mark A, Yildiz, Ahmet

“…Cytoplasmic dynein is a molecular motor responsible for minus-end-directed cargo transport along microtubules (MTs). Dynein motility has previously been…”
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ATF4 Regulates MYB to Increase γ-Globin in Response to Loss of β-Globin by Boontanrart, Mandy Y., Schröder, Markus S., Stehli, Gautier M., Banović, Marija, Wyman, Stacia K., Lew, Rachel J., Bordi, Matteo, Gowen, Benjamin G., DeWitt, Mark A., Corn, Jacob E.

“…β-Hemoglobinopathies can trigger rapid production of red blood cells in a process known as stress erythropoiesis. Cellular stress prompts differentiating…”
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CRISPR-Cas9 interrogation of a putative fetal globin repressor in human erythroid cells by Chung, Jennifer E, Magis, Wendy, Vu, Jonathan, Heo, Seok-Jin, Wartiovaara, Kirmo, Walters, Mark C, Kurita, Ryo, Nakamura, Yukio, Boffelli, Dario, Martin, David I K, Corn, Jacob E, DeWitt, Mark A

“…Sickle Cell Disease and ß-thalassemia, which are caused by defective or deficient adult ß-globin (HBB) respectively, are the most common serious genetic blood…”
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Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells by Shin, Jiyung J., Schröder, Markus S., Caiado, Francisco, Wyman, Stacia K., Bray, Nicolas L., Bordi, Matteo, Dewitt, Mark A., Vu, Jonathan T., Kim, Won-Tae, Hockemeyer, Dirk, Manz, Markus G., Corn, Jacob E.

“…Genome editing often takes the form of either error-prone sequence disruption by non-homologous end joining (NHEJ) or sequence replacement by homology-directed…”
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Genome editing via delivery of Cas9 ribonucleoprotein by DeWitt, Mark A., Corn, Jacob E., Carroll, Dana

“…•CRISPR-Cas genome editing has been employed successfully in many areas of biological research.•Among the remaining challenges in optimizing this platform is…”
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Covalent Protein Labeling and Improved Single-Molecule Optical Properties of Aqueous CdSe/CdS Quantum Dots by Wichner, Sara M, Mann, Victor R, Powers, Alexander S, Segal, Maya A, Mir, Mustafa, Bandaria, Jigar N, DeWitt, Mark A, Darzacq, Xavier, Yildiz, Ahmet, Cohen, Bruce E

“…Semiconductor quantum dots (QDs) have proven to be superior probes for single-molecule imaging compared to organic or genetically encoded fluorophores, but…”
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High-level correction of the sickle mutation is amplified in vivo during erythroid differentiation by Magis, Wendy, DeWitt, Mark A., Wyman, Stacia K., Vu, Jonathan T., Heo, Seok-Jin, Shao, Shirley J., Hennig, Finn, Romero, Zulema G., Campo-Fernandez, Beatriz, Said, Suzanne, McNeill, Matthew S., Rettig, Garrett R., Sun, Yongming, Wang, Yu, Behlke, Mark A., Kohn, Donald B., Boffelli, Dario, Walters, Mark C., Corn, Jacob E., Martin, David I.K.

“…A point mutation in sickle cell disease (SCD) alters one amino acid in the β-globin subunit of hemoglobin, with resultant anemia and multiorgan damage that…”
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The Conformations of the Manganese Transport Regulator of Bacillus subtilis in its Metal-free State by DeWitt, Mark A., Kliegman, Joseph I., Helmann, John D., Brennan, Richard G., Farrens, David L., Glasfeld, Arthur

“…The manganese transport regulator (MntR) from Bacillus subtilis binds cognate DNA sequences in response to elevated manganese concentrations. MntR functions as…”
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Antisense targeting of decoy exons can reduce intron retention and increase protein expression in human erythroblasts by Parra, Marilyn K, Zhang, Weiguo, Vu, Jonathan, DeWitt, Mark A, Conboy, John G

“…The decoy exon model has been proposed to regulate a subset of intron retention (IR) events involving predominantly larger introns (>1kb). Splicing reporter…”
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Novel Quantum Dot Probes for Single-Molecule Biophysics by Wichner, Sara M., DeWitt, Mark A., Cohen, Bruce E., Yildiz, Ahmet

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