Search Results - "DeTitta, George T."

Thermofluor-based high-throughput stability optimization of proteins for structural studies by Ericsson, Ulrika B., Hallberg, B. Martin, DeTitta, George T., Dekker, Niek, Nordlund, Pär

“…Production of proteins well suited for structural studies is inherently difficult and time-consuming. Protein sample homogeneity, stability, and solubility are…”
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A deliberate approach to screening for initial crystallization conditions of biological macromolecules by Luft, Joseph R, Collins, Robert J, Fehrman, Nancy A, Lauricella, Angela M, Veatch, Christina K, DeTitta, George T

“…A method to rationally predict crystallization conditions for a previously uncrystallized macromolecule has not yet been developed. One way around this problem…”
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Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies by Arakaki, Tracy L, Buckner, Frederick S, Gillespie, J. Robert, Malmquist, Nicholas A, Phillips, Margaret A, Kalyuzhniy, Oleksandr, Luft, Joseph R, DeTitta, George T, Verlinde, Christophe L.M.J, Van Voorhis, Wesley C, Hol, Wim G.J, Merritt, Ethan A

“…Nucleotide biosynthesis pathways have been reported to be essential in some protozoan pathogens. Hence, we evaluated the essentiality of one enzyme in the…”
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Blocking S-Adenosylmethionine Synthesis in Yeast Allows Selenomethionine Incorporation and Multiwavelength Anomalous Dispersion Phasing by Malkowski, Michael G., Quartley, Erin, Friedman, Alan E., Babulski, Julie, Kon, Yoshiko, Wolfley, Jennifer, Said, Meriem, Luft, Joseph R., Phizicky, Eric M., DeTitta, George T., Grayhack, Elizabeth J.

“…Saccharomyces cerevisiae is an ideal host from which to obtain high levels of posttranslationally modified eukaryotic proteins for x-ray crystallography…”
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Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data by Hunt, John F, Price II, W Nicholson, Chen, Yang, Handelman, Samuel K, Neely, Helen, Manor, Philip, Karlin, Richard, Nair, Rajesh, Liu, Jinfeng, Baran, Michael, Everett, John, Tong, Saichiu N, Forouhar, Farhad, Swaminathan, Swarup S, Acton, Thomas, Xiao, Rong, Luft, Joseph R, Lauricella, Angela, DeTitta, George T, Rost, Burkhard, Montelione, Gaetano T

“…The physical properties that determine the propensity of a protein to form a well-ordered crystal suitable for structure determination are poorly understood…”
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On the Application of the Minimal Principle to Solve Unknown Structures by Miller, Russ, DeTitta, George T., Jones, Rob, Langs, David A., Weeks, Charles M., Hauptman, Herbert A.

“…The Shake-and-Bake method of structure determination is a new direct methods phasing algorithm based on a minimum-variance, phase invariant residual, which is…”
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Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography by Clark, Kathleen M., Fedoriw, Nadia, Robinson, Katrina, Connelly, Sara M., Randles, Joan, Malkowski, Michael G., DeTitta, George T., Dumont, Mark E.

“…To enhance the quantity and quality of eukaryotic transmembrane proteins (TMPs) available for structure determination by X-ray crystallography, we have…”
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Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins by Koszelak‐Rosenblum, Mary, Krol, Adam, Mozumdar, Namrita, Wunsch, Kristin, Ferin, Adam, Cook, Eleanor, Veatch, Christina K., Nagel, Raymond, Luft, Joseph R., DeTitta, George T., Malkowski, Michael G.

“…Elucidating the structures of membrane proteins is essential to our understanding of disease states and a critical component in the rational design of drugs…”
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Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding by Quartley, Erin, Alexandrov, Andrei, Mikucki, Maryann, Buckner, Frederick S, Hol, Wim G, DeTitta, George T, Phizicky, Eric M, Grayhack, Elizabeth J

“…High level expression of many eukaryotic proteins for structural analysis is likely to require a eukaryotic host since many proteins are either insoluble or…”
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Efficient optimization of crystallization conditions by manipulation of drop volume ratio and temperature by Luft, Joseph R., Wolfley, Jennifer R., Said, Meriem I., Nagel, Raymond M., Lauricella, Angela M., Smith, Jennifer L., Thayer, Max H., Veatch, Christina K., Snell, Edward H., Malkowski, Michael G., DeTitta, George T.

“…An efficient optimization method for the crystallization of biological macromolecules has been developed and tested. This builds on a successful…”
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Macromolecular crystallization in a high throughput laboratory—the search phase by Luft, Joseph R, Wolfley, Jennifer, Jurisica, Igor, Glasgow, Janice, Fortier, Suzanne, DeTitta, George T

“…Macromolecular crystallization efforts are frequently divided into a search phase, during which approximate conditions are sought, and an optimization phase,…”
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The Crystal Structure of MT0146/CbiT Suggests that the Putative Precorrin-8w Decarboxylase Is a Methyltransferase by Keller, Jacob P., Smith, Paul M., Benach, Jordi, Christendat, Dinesh, deTitta, George T., Hunt, John F.

“…The CbiT and CbiE enzymes participate in the biosynthesis of vitamin B12. They are fused together in some organisms to form a protein called CobL, which…”
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Inherent Conformation of the Biotin Bicyclic Moiety: Searching for a Role for Sulfur by DeTitta, George T, Blessing, Robert H, Moss, Grant R, King, Harry F, Sukumaran, Dinesh K, Roskwitalski, Raymond L

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Establishing a training set through the visual analysis of crystallization trials. Part II: crystal examples by Snell, Edward H., Lauricella, Angela M., Potter, Stephen A., Luft, Joseph R., Gulde, Stacey M., Collins, Robert J., Franks, Geoff, Malkowski, Michael G., Cumbaa, Christian, Jurisica, Igor, DeTitta, George T.

“…In the automated image analysis of crystallization experiments, representative examples of outcomes can be obtained rapidly. However, while the outcomes appear…”
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A method to produce microseed stock for use in the crystallization of biological macromolecules by Luft, Joseph R., DeTitta, George T.

“…A method is presented for producing a seed‐stock mixture for macromolecular crystallization. A PTFE bead and micro­centrifuge tube act as mortar and pestle for…”
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Improving objectivity and scalability in protein crystallization by Jurisica, I., Rogers, P., Glasgow, J.I., Collins, R.J., Wolfley, J.R., Luft, J.R., DeTitta, G.T.

“…This article describes the application of image analysis techniques to protein crystallization experiment classification. By applying knowledge discovery…”
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Molecular structure of biotin. Results of two independent crystal structure investigations by DeTitta, George T, Edmonds, James W, Stallings, William, Donohue, Jerry

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High-Resolution X-ray Diffraction and ab Initio Quantum Chemical Studies of Glycoluril, a Biotin Analog by Li, Naiyin, Maluendes, Sergio, Blessing, Robert H, Dupuis, Michel, Moss, Grant R, DeTitta, George T

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Chirality of intermediates in thiamin catalysis: structure of (+)-2-(1-hydroxyethyl)-3,4-dimethyl-5-(2-hydroxyethyl)thiazolium iodide, the absolute stereochemistry of the enantiomers of 2-(1-hydroxyethyl)thiamin, and enzymic reaction of the diphosphates by Kluger, Ronald, Karimian, Khashayar, Gish, Gerald, Pangborn, Walter A, DeTitta, George T

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Preface by DeTitta, George T

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