Search Results - "De Vendittis, E."

Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve by Arcucci, A, Ruocco, M R, Albano, F, Granato, G, Romano, V, Corso, G, Bancone, C, De Vendittis, E, Della Corte, A, Montagnani, S

“…Ascending aortic aneurysm (AsAA) is a consequence of medial degeneration (MD), deriving from apoptotic loss of smooth muscle cells (SMC) and fragmentation of…”
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Psychrophilic superoxide dismutase from Pseudoalteromonas haloplanktis: biochemical characterization and identification of a highly reactive cysteine residue by Castellano, I., Di Maro, A., Ruocco, M.R., Chambery, A., Parente, A., Di Martino, M.T., Parlato, G., Masullo, M., De Vendittis, E.

“…A psychrophilic superoxide dismutase (SOD) has been characterized from the Antarctic eubacterium Pseudoalteromonas haloplanktis ( Ph). PhSOD is a homodimeric…”
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Erratum - Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve by Arcucci, A, Ruocco, M R, Albano, F, Granato, G, Romano, V, Corso, G, Bancone, C, De Vendittis, E, Della Corte, A, Montagnani, S

“…This correct the article published on European Journal of Histochemistry 2014;58:200-206 doi: 10.4081/ejh.2014.2383…”
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Archaebacterial elongation factor 1 alpha carries the catalytic site for GTP hydrolysis by MASULLO, M, DE VENDITTIS, E, BOCCHINI, V

“…The elongation factor 1 alpha from the archaebacterium Sulfolobus solfataricus (aEF-1 alpha) possesses an intrinsic GTPase activity that is triggered by NaCl…”
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The elongation factor G carries a catalytic site for GTP hydrolysis, which is revealed by using 2-propanol in the absence of ribosomes by De Vendittis, E, Masullo, M, Bocchini, V

“…In the absence of ribosomal particles, elongation factor G (EF-G) promotes very little GTP hydrolysis. After the addition of some aliphatic alcohols to EF-G,…”
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Markers of mitochondrial dysfunction during the diclofenac-induced apoptosis in melanoma cell lines by Albano, Francesco, Arcucci, Alessandro, Granato, Giuseppina, Romano, Simona, Montagnani, Stefania, De Vendittis, Emmanuele, Ruocco, Maria Rosaria

“…Melanoma is an aggressive cutaneous cancer, whose incidence is growing in recent years, especially in the younger population. The favorable therapy for this…”
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Heterologous expression in Escherichia coli of the gene encoding an archaeal thermoacidophilic elongation factor 2. Properties of the recombinant protein by De Vendittis, E., Amatruda, M.R., Raimo, G., Bocchini, V.

“…The gene encoding the elongation factor 2 from the hyperthermophilic archaeon Sulfolobus solfataricus ( SsEF-2) was expressed in Escherichia coli using the…”
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Regeneration of the GTP-bound from the GDP-bound form of human and yeast ras proteins by nucleotide exchange. Stimulatory effect of organic and inorganic polyphosphates by Vendittis, E. de, Zahn, R, Fasano, O

“…The regeneration of the GTP-bound from the GDP-bound form of purified human and yeast ras proteins occurs in vitro by a nucleotide-exchange reaction. For both…”
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RAS residues that are distant from the GDP binding site play a critical role in dissociation factor-stimulated release of GDP by Verrotti, A.C, Crechet, J.B, Di Blasi, F, Seidita, G, Mirisola, M.G, Kavounis, C, Nastopoulos, V, Burderi, E, De Vendittis, E, Parmeggiani, A

“…We have previously shown that a conserved glycine at position 82 of the yeast RAS2 protein is involved in the conversion of RAS proteins from the GDP- to the…”
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Hydrolysis of GTP by elongation factor Tu can be induced by monovalent cations in the absence of other effectors by Fasano, O, De Vendittis, E, Parmeggiani, A

“…In this work the authors show that increasing concentrations of monovalent cations cause the gamma -phosphate hydrolysis of the elongation factor Tu…”
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Suppression of defective RAS1 and RAS2 functions in yeast by an adenylate cyclase activated by a single amino acid change by De Vendittis, E., Vitelli, A., Zahn, R., Fasano, O.

“…We have constructed the yeast strain TS1, with the RAS2 gene replaced by mutant allele encoding a partially defective gene product, and with an inactive RAS1…”
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Adaptation of model proteins from cold to hot environments involves continuous and small adjustments of average parameters related to amino acid composition by De Vendittis, Emmanuele, Castellano, Immacolata, Cotugno, Roberta, Ruocco, Maria Rosaria, Raimo, Gennaro, Masullo, Mariorosario

“…The growth temperature adaptation of six model proteins has been studied in 42 microorganisms belonging to eubacterial and archaeal kingdoms, covering optimum…”
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Effect of propan-2-ol on enzymic and structural properties of elongation factor G by Masullo, M, Parlato, G, De Vendittis, E, Bocchini, V

“…Elongation factor G (EF-G) can support a GTPase activity in vitro even in the absence of ribosomes when propan-2-ol is present [GTPasep; De Vendittis, Masullo…”
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Identification of regulatory residues of the yeast adenylyl cyclase by Feger, G., De Vendittis, E., Vitelli, A., Masturzo, P., Zahn, R., Verrotti, A. C., Kavounis, C., Pal, G. P., Fasano, O.

“…We have attempted to identify amino acid residues of the yeast adenylyl cyclase that are involved in the regulation of its activity, by isolating adenylyl…”
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Glucose phosphate isomerase (GPI) "Morcone": a new variant from Italy by Alfinito, F, Ferraro, F, Rocco, S, De Vendittis, E, Piccirillo, G, Sementa, A, Colombo, M B, Zanella, A, Rotoli, B

“…Here we report the 4th Italian case of glucose phosphate isomerase (GPI) deficiency. The propositus is a young man suffering from chronic haemolytic anaemia…”
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Improving protein crystal quality by the without-oil microbatch method: crystallization and preliminary X-ray diffraction analysis of glutathione synthetase from Pseudoalteromonas haloplanktis by Merlino, Antonello, Russo Krauss, Irene, Albino, Antonella, Pica, Andrea, Vergara, Alessandro, Masullo, Mariorosario, De Vendittis, Emmanuele, Sica, Filomena

“…Glutathione synthetases catalyze the ATP-dependent synthesis of glutathione from l-γ-glutamyl- l-cysteine and glycine. Although these enzymes have been…”
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Differential cold-adaptation among protein components of the thioredoxin system in the psychrophilic eubacterium Pseudoalteromonas haloplanktis TAC 125 by Cotugno, Roberta, Rosaria Ruocco, Maria, Marco, Salvatore, Falasca, Patrizia, Evangelista, Giovanna, Raimo, Gennaro, Chambery, Angela, Di Maro, Antimo, Masullo, Mariorosario, De Vendittis, Emmanuele

“…Thioredoxin and thioredoxin reductase from the psychrophilic eubacterium Pseudoalteromonas haloplanktis were obtained as recombinant His-tagged proteins…”
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Yeast mutants temperature‐sensitive for growth after random mutagenesis of the chromosomal RAS2 gene and deletion of the RAS1 gene by Fasano, O., Crechet, J. B., De Vendittis, E., Zahn, R., Feger, G., Vitelli, A., Parmeggiani, A.

“…Saccharomyces cerevisiae strains with a disrupted RAS1 gene and with an intact RAS2 gene (ras1‐ RAS2 strains) grew well on both fermentable and nonfermentable…”
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Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: analysis of structure and thermostability by Ursby, Thomas, Adinolfi, Bianca Stella, Al-Karadaghi, Salam, De Vendittis, Emmanuele, Bocchini, Vincenzo

“…The crystal structure of superoxide dismutase (SOD) from the hyper thermophile Sulfolobus solfataricus has been determined at 2.3 Å resolution by molecular…”
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Structure, stability and flexibility of a psychrophilic iron superoxide dismutase by Merlino, A., Russo Krauss, I., Castellano, I., De Vendittis, E., Vergara, A., Sica, F.

“…Abstract only…”
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