Role of IgG(T) and IgGa isotypes obtained from arachnidic antivenom to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms

The ability of IgG(T) and IgGa subclasses—isolated by liquid chromatography from equine arachnidic antivenom (AAV)—to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms as well as to remove venom toxins from circulation was investigated. These subcl...

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Bibliographic Details
Published in:	Toxicon (Oxford) Vol. 48; no. 6; pp. 649 - 661
Main Authors:	Toro, Ana Flávia, Malta, Marília Brinati, Soares, Sabrina Lucio, Da Rocha, Guilherme Casoni, da Silva Lira, Marcela, De Oliveira, Thais Abbate, Takehara, Harumi Ando, Lopes-Ferreira, Mônica, Santoro, Marcelo Larami, Guidolin, Rosalvo, Gondo Higashi, Hisako, Fernandes, Irene, Barbaro, Katia Cristina
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-11-2006 Elsevier Science
Subjects:	Adeno-associated virus Animal poisons toxicology. Antivenoms Animals Antivenins - chemistry Antivenins - pharmacology Arachnid Arachnidic antivenom Biological and medical sciences Chromatography, Liquid Horses - immunology IgG(T) IgGa Immunoglobulin G - isolation & purification Immunoglobulin G - pharmacology Immunotherapy Loxosceles Loxosceles gaucho Medical sciences Mice Phoneutria Phoneutria nigriventer Phosphoric Diester Hydrolases - immunology Phosphoric Diester Hydrolases - toxicity Scorpion Venoms - antagonists & inhibitors Scorpion Venoms - immunology Scorpion Venoms - toxicity Spider Venoms - antagonists & inhibitors Spider Venoms - immunology Spider Venoms - toxicity Spiders - chemistry Spiders - metabolism Tityus Tityus serrulatus Toxicology Venom Arachnidic antivenom Loxosceles Immunotherapy Venom Arachnid IgG(T) IgGa Phoneutria Tityus Scorpionides Rodentia Animal origin Activity Araneida Arachnida Vertebrata Mammalia Treatment Antivenom Mouse Arthropoda Animal Invertebrata Neutralization
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Summary:	The ability of IgG(T) and IgGa subclasses—isolated by liquid chromatography from equine arachnidic antivenom (AAV)—to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms as well as to remove venom toxins from circulation was investigated. These subclasses showed similar antibody titers against L. gaucho, P. nigriventer and T. serrulatus venoms, and by immunoblotting few differences were observed in the recognition pattern of venom antigens. IgG(T) and IgGa neutralized 100% lethality induced by L. gaucho and 50% of P. nigriventer venom, but IgGa failed to neutralize T. serrulatus venom, in contrast to IgG(T). Both subclasses neutralized local reactions and dermonecrosis induced by L. gaucho venom in rabbits. In mice, IgG(T) and IgGa partially neutralized the edematogenic activity induced by P. nigriventer and T. serrulatus venoms, but only IgG(T) neutralized (ca. 81%) the nociceptive activity induced by T. serrulatus venom. Both subclasses failed to neutralize nociceptive activity induced by P. nigriventer venom. IgG(T) reduced the serum venom levels of animals injected with L. gaucho, P. nigriventer or T. serrulatus venoms, while IgGa solely reduced L. gaucho and P. nigriventer venoms levels. Our results demostrate that IgG(T) and IgGa subclasses neutralize toxic activities induced by P. nigriventer, T. serrulatus and L. gaucho venoms with different efficacies, as well as depurate these venoms from circulation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-0101 1879-3150
DOI:	10.1016/j.toxicon.2006.07.029