Search Results - "De Mol, Nico J"

Optimizing Divalent Inhibitors of Pseudomonas aeruginosa Lectin LecA by Using A Rigid Spacer by Pertici, Francesca, de Mol, Nico J., Kemmink, Johan, Pieters, Roland J.

“…Rigid spacers: Optimal highly potent divalent ligands of the P. aeruginosa lectin LecA have been prepared. The ligands consist of a rigid spacer derived from…”
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Functionalization of a Rigid Divalent Ligand for LecA, a Bacterial Adhesion Lectin by Fu, Ou, Pukin, Aliaksei V., Quarles van Ufford, H. C., Kemmink, Johan, de Mol, Nico J., Pieters, Roland J.

“…The bacterial adhesion lectin LecA is an attractive target for interference with the infectivity of its producer P. aeruginosa. Divalent ligands with two…”
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Site-specific peptide and protein immobilization on surface plasmon resonance chips via strain-promoted cycloaddition by Wammes, Angelique E M, Fischer, Marcel J E, de Mol, Nico J, van Eldijk, Mark B, Rutjes, Floris P J T, van Hest, Jan C M, van Delft, Floris L

“…Surface plasmon resonance (SPR) is a powerful label-free diagnostic tool to study biomolecular interactions. However, one of the drawbacks of SPR is the lack…”
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Synthesis of Lactose Dendrimers and Multivalency Effects in Binding to the Cholera Toxin B Subunit by Vrasidas, Ioannis, de Mol, Nico J., Liskamp, Rob M. J., Pieters, Roland J.

“…The synthesis of lactose‐containing dendrimers is described; the dendrimers used were based on the 3,5‐di(2‐aminoethoxy)benzoic acid repeating unit. Dendrimers…”
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Peptidomimetic ligands for the tandem SH2 domain of the Syk protein involved in signal transduction by Kuil, Joeri, van Wandelen, Loek T M, de Mol, Nico J, Liskamp, Rob M J

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Thiourea-based spacers in potent divalent inhibitors of Pseudomonas aeruginosa virulence lectin LecA by Pukin, Aliaksei V, Brouwer, Arwin J, Koomen, Leonie, Quarles van Ufford, H C, Kemmink, Johan, de Mol, Nico J, Pieters, Roland J

“…A new divalent highly potent inhibitor of the Pseudomonas aeruginosa lectin and virulence factor LecA was prepared. It contains two thiourea linkages which…”
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Surface plasmon resonance: a general introduction by de Mol, Nico J, Fischer, Marcel J E

“…Surface plasmon resonance (SPR) analysis is rather unique in that it allows assay of binding constants (affinity) and kinetic analysis of binding phenomena…”
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Unusual binding of Grb2 protein to a bivalent polyproline-ligand immobilized on a SPR sensor: Intermolecular bivalent binding by de Mol, Nico J., Kruijtzer, John A.W., Moret, Ed E., Broutin, Isabelle, Liskamp, Rob M.J.

“…The Grb2 adapter protein is involved in the activation of the Ras signaling pathway. It recruits the Sos protein by binding of its two SH3 domains to Sos…”
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Affinity constants for small molecules from SPR competition experiments by de Mol, Nico J

“…Direct assay of small molecules by SPR in general is troublesome and at least tedious procedures have to be applied. Competition experiments offer an…”
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ITAM-derived phosphopeptide-containing dendrimers as multivalent ligands for Syk tandem SH2 domain by Kuil, Joeri, Branderhorst, Hilbert M, Pieters, Roland J, de Mol, Nico J, Liskamp, Rob M J

“…Spleen tyrosine kinase (Syk) is activated when its tandem SH2 domain (tSH2) binds to a diphosphorylated ITAM motif of e.g. the FcepsilonRI receptor. In this…”
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Cell permeable ITAM constructs for the modulation of mediator release in mast cells by Kuil, Joeri, Fischer, Marcel J E, de Mol, Nico J, Liskamp, Rob M J

“…Spleen tyrosine kinase (Syk) is essential for high affinity IgE receptor (FcεRI) mediated mast cell degranulation. Once FcεRI is stimulated, intracellular ITAM…”
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Tissue-type plasminogen activator binds to Aβ and AIAPP amyloid fibrils with multiple domains by Beringer, Dennis X., Fischer, Marcel J. E., Meeldijk, Johannes D., van Donselaar, Elly G., de Mol, Nico J., Kroon-Batenburg, Loes M. J.

“…Abstract Binding of tissue-type plasminogen activator (tPA) to amyloid and denatured proteins is reported in a number of studies. The binding site has been…”
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Switching between low and high affinity for the Syk tandem SH2 domain by irradiation of azobenzene containing ITAM peptidomimetics by Kuil, Joeri, van Wandelen, Loek T.M., de Mol, Nico J., Liskamp, Rob M.J.

“…Spleen tyrosine kinase (Syk) plays an essential role in IgE receptor signaling (FcεRI), which leads to mast cell degranulation. Divalent binding of the tandem…”
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Surface plasmon resonance for proteomics by de Mol, Nico J

“…Surface plasmon resonance (SPR) is a well-established label-free technique to detect mass changes near an SPR surface. For 20 years the benefits of SPR have…”
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Protein Flexibility and Ligand Rigidity: A Thermodynamic and Kinetic Study of ITAM-Based Ligand Binding to Syk Tandem SH2 by de Mol, Nico J, Catalina, M. Isabel, Dekker, Frank J, Fischer, Marcel J.E, Heck, Albert J.R, Liskamp, Rob M.J

“…The Syk tandem Src homology 2 domain (Syk tSH2) constitutes a flexible protein module involved in the regulation of Syk kinase activity. The Syk tSH2 domain is…”
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Effects of oxatomide and derivatives on high affinity IgE receptor-activated signal transduction pathways in rat basophilic leukemia cells: role of protein tyrosine hyperphosphorylation and inhibition of extracellular calcium influx by Paulussen, Jeannette J.C, Fischer, Marcel J.E, Roozendaal, Ramon L, van der Heijden, Vincent C, van Dijken, Peter, de Mol, Nico J, Janssen, Lambert H.M

“…The antiallergic drug oxatomide and analogs inhibit mediator release from a rat basophilic leukemia (RBL-2H3) cell line, which is frequently used as a mast…”
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Cyclic phosphopeptides for interference with Grb2 SH2 domain signal transduction prepared by ring-closing metathesis and phosphorylation by Dekker, Frank J, de Mol, Nico J, Fischer, Marcel J E, Kemmink, Johan, Liskamp, Rob M J

“…Cyclic phosphopeptides were prepared using ring-closing metathesis followed by phosphorylation. These cyclic phosphopeptides were designed to interact with the…”
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Changes in structural dynamics of the Grb2 adaptor protein upon binding of phosphotyrosine ligand to its SH2 domain by de Mol, Nico J., Catalina, M.Isabel, Fischer, Marcel J.E., Broutin, Isabelle, Maier, Claudia S., Heck, Albert J.R.

“…Growth factor receptor-bound protein 2 (Grb2) is an extensively studied adaptor protein involved in cell signaling. Grb2 is a highly flexible protein composed…”
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Replacement of the Intervening Amino Acid Sequence of a Syk-Binding Diphosphopeptide by a Nonpeptide Spacer with Preservation of High Affinity by Dekker, Frank J., de Mol, Nico J., van Ameijde, Jeroen, Fischer, Marcel J. E., Ruijtenbeek, Rob, Redegeld, Frank A. M., Liskamp, Rob M. J.

“…Divalent peptidomimetic compounds can bind a signal transduction protein and provide an avenue towards uncovering compounds capable of influencing or…”
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Amino propynyl benzoic acid building block in rigid spacers of divalent ligands binding to the syk SH2 domains with equally high affinity as the natural ligand by Dekker, Frank J, de Mol, Nico J, Fischer, Marcel J.E, Liskamp, Rob M.J

“…The construction of rigid spacers composed of amino propynyl benzoic acid building blocks is described. These spacers were used to link two phosphopeptide…”
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