A network system for vitellogenin synthesis in the mussel Mytilus galloprovincialis (L.)

The aim of this study is to assess, by RT‐PCR, in situ hybridization, electron microscopy, and immunohistochemistry, the site/s of vitellogenin (VTG) synthesis in the mussel Mytilus galloprovincialis. Our investigations demonstrate that, among the analyzed tissues, the synthesis of VTG occurs only i...

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Published in:	Journal of cellular physiology Vol. 228; no. 3; pp. 547 - 555
Main Authors:	Agnese, Marisa, Verderame, Mariailaria, De Meo, Enrico, Prisco, Marina, Rosati, Luigi, Limatola, Ermelinda, del Gaudio, Rosanna, Aceto, Serena, Andreuccetti, Piero
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-03-2013 Wiley Subscription Services, Inc
Subjects:	Animals Digestive glands Female Immunohistochemistry In Situ Hybridization Microscopy, Electron, Transmission Mollusks Mytilus - genetics Mytilus - metabolism Mytilus - ultrastructure Oocytes - metabolism Oocytes - ultrastructure Ovary - anatomy & histology Ovary - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Vitellogenins - biosynthesis Vitellogenins - genetics
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Summary:	The aim of this study is to assess, by RT‐PCR, in situ hybridization, electron microscopy, and immunohistochemistry, the site/s of vitellogenin (VTG) synthesis in the mussel Mytilus galloprovincialis. Our investigations demonstrate that, among the analyzed tissues, the synthesis of VTG occurs only in the female gonad, that is, within the oocyte and follicle and connective cells. Such a synthesis is just evident in early vitellogenic oocytes, whose cytoplasm is characterized by numerous RER cisternae and an extended Golgi complex surrounded by nascent yolk platelets. The synthesis of VTG goes on in vitellogenic oocytes assuming a pear form, and progressively reduces once the oocyte shows the pear or polygonal form, typical of those oocytes that have concluded the growth. The expression of VTG occurs also within follicle (auxiliary) and connective cells. In particular, it is noteworthy that follicle cells are characterized by numerous RER cisternae and an active Golgi complex surrounded by numerous vesicles and vacuoles containing electron dense material. The same material is also present along their plasma membrane, within the intercellular space between oocyte and follicle cells, and finally within invaginations of the oocyte surface, thus suggesting a VTG transfer to the oocyte via endocytosis. Differently, no VTG synthesis was observed within digestive gland. All together the findings here reported strongly suggest that in M. galloprovincialis, inside the gonad, the VTG synthesis occurs in the oocyte (autosynthesis) and in the follicle and adipogranular cells (heterosynthesis). J. Cell. Physiol. 228: 547–555, 2013. © 2012 Wiley Periodicals, Inc.
Bibliography:	MIUR PRIN 2010-2011 to Prof G. De Vico istex:278F197A82B4C5C173DDCB20C8C33AEB8706C226 University of Naples Federico II (F.A.R.O 2011 to Dr. S. Aceto) ArticleID:JCP24161 ark:/67375/WNG-K465N5S5-T ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9541 1097-4652
DOI:	10.1002/jcp.24161