Search Results - "De Lorenzo, Víctor"

Bioremediation 3.0: Engineering pollutant-removing bacteria in the times of systemic biology by Dvořák, Pavel, Nikel, Pablo I., Damborský, Jiří, de Lorenzo, Víctor

“…Elimination or mitigation of the toxic effects of chemical waste released to the environment by industrial and urban activities relies largely on the catalytic…”
Get full text

In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9 by Álvarez, Beatriz, Mencía, Mario, de Lorenzo, Víctor, Fernández, Luis Ángel

“…In vivo mutagenesis systems accelerate directed protein evolution but often show restricted capabilities and deleterious off-site mutations on cells. To…”
Get full text

Volatilization of Arsenic from Polluted Soil by Pseudomonas putida Engineered for Expression of the arsM Arsenic(III) S‑Adenosine Methyltransferase Gene by Chen, Jian, Sun, Guo-Xin, Wang, Xiao-Xue, Lorenzo, Víctor de, Rosen, Barry P, Zhu, Yong-Guan

“…Even though arsenic is one of the most widespread environmental carcinogens, methods of remediation are still limited. In this report we demonstrate that a…”
Get full text

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis by Belda, Eugeni, van Heck, Ruben G. A., José Lopez-Sanchez, Maria, Cruveiller, Stéphane, Barbe, Valérie, Fraser, Claire, Klenk, Hans-Peter, Petersen, Jörn, Morgat, Anne, Nikel, Pablo I., Vallenet, David, Rouy, Zoé, Sekowska, Agnieszka, Martins dos Santos, Vitor A. P., de Lorenzo, Víctor, Danchin, Antoine, Médigue, Claudine

“…Summary By the time the complete genome sequence of the soil bacterium Pseudomonas putida KT2440 was published in 2002 (Nelson et al., ) this bacterium was…”
Get full text

Chemical reactivity drives spatiotemporal organisation of bacterial metabolism by de Lorenzo, Víctor, Sekowska, Agnieszka, Danchin, Antoine

“…In this review, we examine how bacterial metabolism is shaped by chemical constraints acting on the material and dynamic layout of enzymatic networks and…”
Get full text

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities by Martínez-García, Esteban, Aparicio, Tomás, Goñi-Moreno, Angel, Fraile, Sofía, de Lorenzo, Víctor

“…The Standard European Vector Architecture 2.0 database (SEVA-DB 2.0, http://seva.cnb.csic.es) is an improved and expanded version of the platform released in…”
Get full text

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes by Silva-Rocha, Rafael, Martínez-García, Esteban, Calles, Belén, Chavarría, Max, Arce-Rodríguez, Alejandro, de Las Heras, Aitor, Páez-Espino, A David, Durante-Rodríguez, Gonzalo, Kim, Juhyun, Nikel, Pablo I, Platero, Raúl, de Lorenzo, Víctor

“…The 'Standard European Vector Architecture' database (SEVA-DB, http://seva.cnb.csic.es) was conceived as a user-friendly, web-based resource and a material…”
Get full text

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway by Dvorak, Pavel, Chrast, Lukas, Nikel, Pablo I, Fedr, Radek, Soucek, Karel, Sedlackova, Miroslava, Chaloupkova, Radka, de Lorenzo, Víctor, Prokop, Zbynek, Damborsky, Jiri

“…Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate…”
Get full text

Versioning biological cells for trustworthy cell engineering by Tellechea-Luzardo, Jonathan, Hobbs, Leanne, Velázquez, Elena, Pelechova, Lenka, Woods, Simon, de Lorenzo, Víctor, Krasnogor, Natalio

“…“Full-stack” biotechnology platforms for cell line (re)programming are on the horizon, thanks mostly to (a) advances in gene synthesis and editing techniques…”
Get full text

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes by Martínez-García, Esteban, Fraile, Sofía, Algar, Elena, Aparicio, Tomás, Velázquez, Elena, Calles, Belén, Tas, Huseyin, Blázquez, Blas, Martín, Bruno, Prieto, Clara, Sánchez-Sampedro, Lucas, Nørholm, Morten H H, Volke, Daniel C, Wirth, Nicolas T, Dvořák, Pavel, Alejaldre, Lorea, Grozinger, Lewis, Crowther, Matthew, Goñi-Moreno, Angel, Nikel, Pablo I, Nogales, Juan, de Lorenzo, Víctor

“…The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic…”
Get full text

Engineering the Soil Bacterium Pseudomonas putida for Arsenic Methylation by Chen, Jian, Qin, Jie, Zhu, Yong-Guan, de Lorenzo, Víctor, Rosen, Barry P

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
Get full text

Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors by Martínez-García, Esteban, Aparicio, Tomás, de Lorenzo, Víctor, Nikel, Pablo I

“…The construction of microbial cell factories à la carte largely depends on specialized molecular biology and synthetic biology tools needed to reprogram…”
Get more information

The Metabolic Redox Regime of Pseudomonas putida Tunes Its Evolvability toward Novel Xenobiotic Substrates by Akkaya, Özlem, Pérez-Pantoja, Danilo R, Calles, Belén, Nikel, Pablo I, de Lorenzo, Víctor

“…During evolution of biodegradation pathways for xenobiotic compounds involving Rieske nonheme iron oxygenases, the transition toward novel substrates is…”
Get full text

Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose by Dvořák, Pavel, Burýšková, Barbora, Popelářová, Barbora, Ebert, Birgitta E., Botka, Tibor, Bujdoš, Dalimil, Sánchez-Pascuala, Alberto, Schöttler, Hannah, Hayen, Heiko, de Lorenzo, Víctor, Blank, Lars M., Benešík, Martin

“…To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive…”
Get full text

pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes by Martínez-García, Esteban, Calles, Belén, Arévalo-Rodríguez, Miguel, de Lorenzo, Víctor

“…Since publication in 1977 of plasmid pBR322, many breakthroughs in Biology have depended on increasingly sophisticated vector platforms for analysis and…”
Get full text

The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas putida by Arce-Rodríguez, Alejandro, Calles, Belén, Nikel, Pablo I., de Lorenzo, Víctor

“…Summary The natural physiological regime of the soil bacterium Pseudomonas putida involves incessant exposure to endogenous metabolic conflicts and…”
Get full text

Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery by Velázquez, Elena, Lorenzo, Víctor de, Al-Ramahi, Yamal

“…Group II introns were developed some time ago as tools for the construction of knockout mutants in a wide range of organisms, ranging from Gram-positive and…”
Get full text

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts by Martínez-García, Esteban, Goñi-Moreno, Angel, Bartley, Bryan, McLaughlin, James, Sánchez-Sampedro, Lucas, del Pozo, Héctor Pascual, Hernández, Clara Prieto, Marletta, Ada Serena, De Lucrezia, Davide, Sánchez-Fernández, Guzmán, Fraile, Sofía, de Lorenzo, Víctor

Get full text

The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor by Nikel, Pablo I, Romero-Campero, Francisco J, Zeidman, Joshua A, Goñi-Moreno, Ángel, de Lorenzo, Víctor

“…The growth of the soil bacterium Pseudomonas putida KT2440 on glycerol as the sole carbon source is characterized by a prolonged lag phase, not observed with…”
Get full text

Multiple-Site Diversification of Regulatory Sequences Enables Interspecies Operability of Genetic Devices by Hueso-Gil, Angeles, Nyerges, Ákos, Pál, Csaba, Calles, Belén, de Lorenzo, Víctor

“…The features of the light-responsive cyanobacterial CcaSR regulatory module that determine interoperability of this optogenetic device between Escherichia coli…”
Get full text