Rapid biofabrication of tubular tissue constructs by centrifugal casting in a decellularized natural scaffold with laser-machined micropores

Centrifugal casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by centrifugal casting and...

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Bibliographic Details
Published in:	Journal of materials science. Materials in medicine Vol. 20; no. 1; pp. 329 - 337
Main Authors:	Kasyanov, Vladimir A., Hodde, Jason, Hiles, Michael C., Eisenberg, Carol, Eisenberg, Leonard, De Castro, Luis E. F., Ozolanta, Iveta, Murovska, Modra, Draughn, Robert A., Prestwich, Glenn D., Markwald, Roger R., Mironov, Vladimir
Format:	Journal Article
Language:	English
Published:	Boston Springer US 2009 Springer Nature B.V
Subjects:	Animals Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Blood Vessel Prosthesis Cell Line Cell Survival Ceramics Chemistry and Materials Science Composites Glass Humans Hydrogels Intestinal Mucosa - blood supply Intestinal Mucosa - chemistry Kinetics Lasers Materials Science Materials Testing Mesoderm - cytology Natural Materials Polymer Sciences Quail Regenerative Medicine/Tissue Engineering Surfaces and Interfaces Tensile Strength Thin Films Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry Vascular Graft Ultimate Strain Small Intestinal Submucosa Ultimate Stress Endothelial Progenitor Cell
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Summary:	Centrifugal casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by centrifugal casting and increase hydrogel retention, without compromising the biomechanical properties of the scaffold. Micropores with diameters of 50, 100, and 200 μm were machined at different linear densities in decellularized small intestine submucosa (SIS) planar sheets and tubular SIS scaffolds using an argon laser. The ultimate stress and ultimate strain values for SIS sheets with laser-machined micropores with diameter 50 μm and distance between holes as low as 714 μm were not significantly different from unmachined control SIS specimens. Centrifugal casting of GFP-labeled cells suspended in an in situ cross-linkable hyaluronan-based hydrogel resulted in scaffold recellularization with a high density of viable cells inside the laser-machined micropores. Perfusion tests demonstrated the retention of the cells encapsulated within the HA hydrogel in the microholes. Thus, an SIS scaffold with appropriately sized microholes can be loaded with hydrogel encapsulated cells by centrifugal casting to give a mechanically robust construct that retains the cell-seeded hydrogel, permitting rapid biofabrication of tubular tissue construct in a “bioreactor-free” fashion.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0957-4530 1573-4838
DOI:	10.1007/s10856-008-3590-3