Gene expression profiling in the deep water horse mussel Modiolus modiolus (L.) located near a marine municipal wastewater outfall

The marine bivalve Modiolus modiolus (L.) is a sentinel species used for the assessment of potential biological exposure to anthropogenic contaminants in benthic environments. Herein, we have developed real-time quantitative polymerase chain reaction assays for 12 specific mRNAs. The levels of each...

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Bibliographic Details
Published in:	Aquatic toxicology Vol. 93; no. 2; pp. 116 - 124
Main Authors:	Veldhoen, Nik, Lowe, Christopher J., Davis, Céline, Mazumder, Asit, Helbing, Caren C.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 28-06-2009 Amsterdam; New York: Elsevier Science Elsevier
Subjects:	Animal and plant ecology Animal, plant and microbial ecology Animals Applied ecology Base Sequence Biological and medical sciences Bivalve Bivalvia DNA primers Ecotoxicology, biological effects of pollution effluents Environmental contaminant Environmental Monitoring Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling General aspects genotoxicity Marine messenger RNA Modiolus Modiolus modiolus Molecular Sequence Data mRNA mussels Mytilidae - classification Mytilidae - genetics Mytilidae - metabolism nucleotide sequences Oceans and Seas pollutants polymerase chain reaction Real-time PCR RNA, Messenger - metabolism Sea water ecosystems Seawater - chemistry Synecology toxicity transcription (genetics) Waste Disposal, Fluid Wastewater water pollution British Columbia Environmental contaminant Real-time PCR mRNA Gene expression Wastewater Modiolus Bivalve Ecotoxicology Toxicity Deep water Waste water Marine environment Polymerase chain reaction Aquatic environment Pollution Messenger RNA Bivalvia Environment Urban waste water Invertebrata Mollusca Contaminant
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Summary:	The marine bivalve Modiolus modiolus (L.) is a sentinel species used for the assessment of potential biological exposure to anthropogenic contaminants in benthic environments. Herein, we have developed real-time quantitative polymerase chain reaction assays for 12 specific mRNAs. The levels of each mRNA transcript were evaluated in adductor muscle, gonad, and hepatopancreas tissue collected from animals located at a reference site and a site near a preliminary municipal wastewater treatment outfall. Significant differences in mRNA abundance in animals located at the wastewater outfall site were observed for CAT and NET/SCF6 in all three tissues examined, ABCA4 and HSP70 transcript abundance were increased in the adductor muscle and hepatopancreas, respectively. Transcript levels for MDR, CYP4, rpS4, rpS9, and Ca 2+-ATPase were not different between sites in the three tissues examined. This study sets the foundation for further evaluation of these transcripts’ utility in the evaluation of effluent effects within the marine environment.
Bibliography:	http://dx.doi.org/10.1016/j.aquatox.2009.04.002 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0166-445X 1879-1514
DOI:	10.1016/j.aquatox.2009.04.002