Search Results - "Davis, Brigid M."

The design and analysis of transposon insertion sequencing experiments by Chao, Michael C., Abel, Sören, Davis, Brigid M., Waldor, Matthew K.

“…Transposon insertion sequencing (TIS) enables genome-wide definition of loci that are required for growth in diverse conditions. In this article, Waldor and…”
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ARTIST: high-resolution genome-wide assessment of fitness using transposon-insertion sequencing by Pritchard, Justin R, Chao, Michael C, Abel, Sören, Davis, Brigid M, Baranowski, Catherine, Zhang, Yanjia J, Rubin, Eric J, Waldor, Matthew K

“…Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic requirements for bacterial growth in different conditions, as it enables…”
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Insights into Vibrio cholerae intestinal colonization from monitoring fluorescently labeled bacteria by Millet, Yves A, Alvarez, David, Ringgaard, Simon, von Andrian, Ulrich H, Davis, Brigid M, Waldor, Matthew K

“…Vibrio cholerae, the agent of cholera, is a motile non-invasive pathogen that colonizes the small intestine (SI). Most of our knowledge of the processes…”
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Analysis of Bottlenecks in Experimental Models of Infection by Abel, Sören, Abel zur Wiesch, Pia, Davis, Brigid M, Waldor, Matthew K

“…  [...]genome-wide transposon insertion studies can be confounded by the existence of tight bottlenecks, which can limit the complexity of libraries that can…”
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Inflammation and disintegration of intestinal villi in an experimental model for Vibrio parahaemolyticus-induced diarrhea by Ritchie, Jennifer M, Rui, Haopeng, Zhou, Xiaohui, Iida, Tetsuya, Kodoma, Toshio, Ito, Susuma, Davis, Brigid M, Bronson, Roderick T, Waldor, Matthew K

“…Vibrio parahaemolyticus is a leading cause of seafood-borne gastroenteritis in many parts of the world, but there is limited knowledge of the pathogenesis of…”
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A cell wall damage response mediated by a sensor kinase/response regulator pair enables beta-lactam tolerance by Dörr, Tobias, Alvarez, Laura, Delgado, Fernanda, Davis, Brigid M., Cava, Felipe, Waldor, Matthew K.

“…The bacterial cell wall is critical for maintenance of cell shape and survival. Following exposure to antibiotics that target enzymes required for cell wall…”
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A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole by Yamaichi, Yoshiharu, Bruckner, Raphael, Ringgaard, Simon, Möll, Andrea, Cameron, D Ewen, Briegel, Ariane, Jensen, Grant J, Davis, Brigid M, Waldor, Matthew K

“…The cell poles constitute key subcellular domains that are often critical for motility, chemotaxis, and chromosome segregation in rod-shaped bacteria. However,…”
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Endopeptidase-mediated beta lactam tolerance by Dörr, Tobias, Davis, Brigid M, Waldor, Matthew K

“…In many bacteria, inhibition of cell wall synthesis leads to cell death and lysis. The pathways and enzymes that mediate cell lysis after exposure to cell…”
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A family of ParA-like ATPases promotes cell pole maturation by facilitating polar localization of chemotaxis proteins by Ringgaard, Simon, Schirner, Kathrin, Davis, Brigid M, Waldor, Matthew K

“…Stochastic processes are thought to mediate localization of membrane-associated chemotaxis signaling clusters in peritrichous bacteria. Here, we identified a…”
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Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization by Warr, Alyson R, Hubbard, Troy P, Munera, Diana, Blondel, Carlos J, Abel Zur Wiesch, Pia, Abel, Sören, Wang, Xiaoxue, Davis, Brigid M, Waldor, Matthew K

“…Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is an important food-borne pathogen that colonizes the colon. Transposon-insertion sequencing (TIS) was used…”
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A hybrid approach for the automated finishing of bacterial genomes by Bashir, Ali, Klammer, Aaron A, Robins, William P, Chin, Chen-Shan, Webster, Dale, Paxinos, Ellen, Hsu, David, Ashby, Meredith, Wang, Susana, Peluso, Paul, Sebra, Robert, Sorenson, Jon, Bullard, James, Yen, Jackie, Valdovino, Marie, Mollova, Emilia, Luong, Khai, Lin, Steven, LaMay, Brianna, Joshi, Amruta, Rowe, Lori, Frace, Michael, Tarr, Cheryl L, Turnsek, Maryann, Davis, Brigid M, Kasarskis, Andrew, Mekalanos, John J, Waldor, Matthew K, Schadt, Eric E

“…The multikilobase reads that can be produced by single-molecule sequencing technologies may span complex, repetitive genomic regions but have high error rates…”
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Cell Separation in Vibrio cholerae Is Mediated by a Single Amidase Whose Action Is Modulated by Two Nonredundant Activators by Möll, Andrea, Dörr, Tobias, Alvarez, Laura, Chao, Michael C, Davis, Brigid M, Cava, Felipe, Waldor, Matthew K

“…Synthesis and hydrolysis of septal peptidoglycan (PG) are critical processes at the conclusion of cell division that enable separation of daughter cells…”
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CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity by Blondel, Carlos J., Park, Joseph S., Hubbard, Troy P., Pacheco, Alline R., Kuehl, Carole J., Walsh, Michael J., Davis, Brigid M., Gewurz, Benjamin E., Doench, John G., Waldor, Matthew K.

“…Type III secretion systems (T3SSs) inject bacterial effector proteins into host cells and underlie the virulence of many gram-negative pathogens. Studies have…”
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Comparative RNA-Seq based dissection of the regulatory networks and environmental stimuli underlying Vibrio parahaemolyticus gene expression during infection by Livny, Jonathan, Zhou, Xiaohui, Mandlik, Anjali, Hubbard, Troy, Davis, Brigid M, Waldor, Matthew K

“…Vibrio parahaemolyticus is the leading worldwide cause of seafood-associated gastroenteritis, yet little is known regarding its intraintestinal gene expression…”
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Time-Resolved Transposon Insertion Sequencing Reveals Genome-Wide Fitness Dynamics during Infection by Yang, Guanhua, Billings, Gabriel, Hubbard, Troy P, Park, Joseph S, Yin Leung, Ka, Liu, Qin, Davis, Brigid M, Zhang, Yuanxing, Wang, Qiyao, Waldor, Matthew K

“…Transposon insertion sequencing (TIS) is a powerful high-throughput genetic technique that is transforming functional genomics in prokaryotes, because it…”
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CRISPR Screen Reveals that EHEC's T3SS and Shiga Toxin Rely on Shared Host Factors for Infection by Pacheco, Alline R, Lazarus, Jacob E, Sit, Brandon, Schmieder, Stefanie, Lencer, Wayne I, Blondel, Carlos J, Doench, John G, Davis, Brigid M, Waldor, Matthew K

“…Enterohemorrhagic (EHEC) has two critical virulence factors-a type III secretion system (T3SS) and Shiga toxins (Stxs)-that are required for the pathogen to…”
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High-resolution genetic analysis of the requirements for horizontal transmission of the ESBL plasmid from Escherichia coli O104:H4 by Yamaichi, Yoshiharu, Chao, Michael C, Sasabe, Jumpei, Clark, Lars, Davis, Brigid M, Yamamoto, Nozomi, Mori, Hiroshi, Kurokawa, Ken, Waldor, Matthew K

“…Horizontal dissemination of the genes encoding extended spectrum beta-lactamases (ESBLs) via conjugative plasmids is facilitating the increasingly widespread…”
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Substrate specificity of an elongation‐specific peptidoglycan endopeptidase and its implications for cell wall architecture and growth of Vibrio cholerae by Dörr, Tobias, Cava, Felipe, Lam, Hubert, Davis, Brigid M., Waldor, Matthew K.

“…Summary The bacterial cell wall consists of peptidoglycan (PG), a sturdy mesh of glycan strands cross‐linked by short peptides. This rigid structure constrains…”
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ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor by Ringgaard, Simon, Zepeda-Rivera, Martha, Wu, Xiaoji, Schirner, Kathrin, Davis, Brigid M, Waldor, Matthew K

“…Bacterial chemotaxis proteins are organized into ordered arrays. In peritrichous organisms, such as Escherichia coli , stochastic assembly processes are…”
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A Vibrio parahaemolyticus T3SS Effector Mediates Pathogenesis by Independently Enabling Intestinal Colonization and Inhibiting TAK1 Activation by Zhou, Xiaohui, Gewurz, Benjamin E., Ritchie, Jennifer M., Takasaki, Kaoru, Greenfeld, Hannah, Kieff, Elliott, Davis, Brigid M., Waldor, Matthew K.

“…Vibrio parahaemolyticus type III secretion system 2 (T3SS2) is essential for the organism’s virulence, but the effectors required for intestinal colonization…”
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