Association of genetic polymorphism at tumor necrosis factor-α gene promoter - 1031T/C and parasitic infections among children in Northern South Africa

Association of genetic polymorphism at tumor necrosis factor-α gene promoter - 1031T/C and parasitic infections among children in Northern South Africa

Intestinal parasitic diseases are common in developing countries including South Africa and have been documented to be the most common in children under the age of five. The present study aimed to identify any potential association that may exist between TNF-α promoter gene polymorphism and parasiti...

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Bibliographic Details
Published in:Heliyon Vol. 6; no. 10; p. e05129
Main Authors: Davhana, N.C., ElBakri, A.K., Bessong, P.O., Samie, A.
Format: Journal Article
Language:English
Published: Elsevier Ltd 01-10-2020
Elsevier
Subjects:
Dzimauli community
Genetics
Immunology
Limpopo province
Microbiology
Molecular biology
PCR-RFLP
South Africa
TNF-α
Immunology
Microbiology
TNF-α
Dzimauli community
Genetics
South Africa
PCR-RFLP
Molecular biology
Limpopo province
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Summary:Intestinal parasitic diseases are common in developing countries including South Africa and have been documented to be the most common in children under the age of five. The present study aimed to identify any potential association that may exist between TNF-α promoter gene polymorphism and parasitic infections. A total of 199 blood samples were evaluated from children who were part of the MAL-ED study cohort. The DNA was used to investigate polymorphism in the promoter region of the TNF-α gene at position -1031T/C. The polymorphisms were detected by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) assay. The TC genotype at position -1031 was significantly higher in healthy controls children than in children who were infected with Entamoeba species (59.9% vs 29.4%, P = 0.015) and Entamoeba coli (59.1% vs 30.8%, P = 0.046), indicating that TC genotype may be protective against Entamoeba infections and Entamoeba coli infections. The CC genotype at position -1031 was more common among children with parasite and diarrhea and the results was statistically significant (P = 0.04). This study has revealed that the CC genotype may be is a risk factor for symptomatic parasitic infections while the TC genotype might be protective of Entamoeba infections among children in Dzimauli community. Genetics; Immunology; Microbiology; Molecular Biology; TNF-α; PCR-RFLP; Dzimauli community; Limpopo Province; South Africa
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ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2020.e05129