Synthetic Clonal Reproduction Through Seeds

Cloning through seeds has potential revolutionary applications in agriculture, because it would allow vigorous hybrids to be propagated indefinitely. However, asexual seed formation or apomixis, avoiding meiosis and fertilization, is not found in the major food crops. To develop de novo synthesis of...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) Vol. 331; no. 6019; p. 876
Main Authors:	Marimuthu, Mohan P.A, Jolivet, Sylvie, Ravi, Maruthachalam, Pereira, Lucie, Davda, Jayeshkumar N, Cromer, Laurence, Wang, Lili, Nogué, Fabien, Chan, Simon W.L, Siddiqi, Imran, Mercier, Raphaël
Format:	Journal Article
Language:	English
Published:	Washington, DC American Association for the Advancement of Science 18-02-2011 The American Association for the Advancement of Science American Association for the Advancement of Science (AAAS)
Subjects:	Agronomy. Soil science and plant productions Apomixis Arabidopsis - genetics Arabidopsis - physiology Biological and medical sciences BREVIA Cellular Biology Chromosome Segregation - genetics Chromosomes Chromosomes, Plant Cloning Conversion Crosses, Genetic Diploidy Dyadic relations Fertilization Foods Fundamental and applied biological sciences. Psychology Gametes Genes Genes, Plant Genetic Engineering Genetic loci Genetics and breeding of economic plants Genomes Heterozygote Histones - genetics Life Sciences Meiosis - genetics Plant breeding: fundamental aspects and methodology Plant Proteins - genetics Plant reproduction Plants Plants, Genetically Modified Progeny Reproduction Reproduction, Asexual Seeds Seeds - genetics Seeds - physiology Arabidopsis thaliana Seeds Cruciferae Dicotyledones Angiospermae Apomixis Chromosome number Unreduced gamete Spermatophyta Experimental plant Sexual reproduction Clonal propagation clonal female fertilization clonal reproduction chromosomes application en agriculture male gametes GENETIQUE VEGETALE
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Summary:	Cloning through seeds has potential revolutionary applications in agriculture, because it would allow vigorous hybrids to be propagated indefinitely. However, asexual seed formation or apomixis, avoiding meiosis and fertilization, is not found in the major food crops. To develop de novo synthesis of apomixis, we crossed Arabidopsis MiMe and dyad mutants that produce diploid clonal gametes to a strain whose chromosomes are engineered to be eliminated after fertilization. Up to 34% of the progeny were clones of their parent, demonstrating the conversion of clonal female or male gametes into seeds. We also show that first-generation cloned plants can be cloned again. Clonal reproduction through seeds can therefore be achieved in a sexual plant by manipulating two to four conserved genes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1199682