Effects of Adiponectin Including Reduction of Androstenedione Secretion and Ovarian Oxidative Stress Parameters In Vivo

Adiponectin is the most abundantly produced human adipokine with anti-inflammatory, anti-oxidative, and insulin-sensitizing properties. Evidence from in vitro studies has indicated that adiponectin has a potential role in reproduction because it reduces the production of androstenedione in bovine th...

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Published in:	PloS one Vol. 11; no. 5; p. e0154453
Main Authors:	Comim, Fabio V, Gutierrez, Karina, Bridi, Alessandra, Bochi, Guilherme, Chemeris, Raisa, Rigo, Melânia L, Dau, Andressa Minussi P, Cezar, Alfredo S, Moresco, Rafael Noal, Gonçalves, Paulo Bayard Dias
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 09-05-2016 Public Library of Science (PLoS)
Subjects:	Adiponectin Adiponectin - pharmacology Analysis Androstenedione Androstenedione - secretion Animals Biochemistry Biology and Life Sciences Biotechnology Care and treatment Complications and side effects Enzyme-Linked Immunosorbent Assay Enzymes Female Gene expression Gonadotropins Hypotheses In vivo methods and tests Inflammation Influence Insulin Kinases Laboratories Medicine and Health Sciences Mice Mice, Inbred BALB C Ovarian cancer Ovary - drug effects Ovary - metabolism Oxidation Oxidative Stress Phosphates Pituitary (anterior) Polycystic ovary syndrome Proteins Real-Time Polymerase Chain Reaction Recovery (Medical) Reduction Research and Analysis Methods Rodents Secretion Sensitizing Serum levels Studies Theca Brazil United States > US
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Summary:	Adiponectin is the most abundantly produced human adipokine with anti-inflammatory, anti-oxidative, and insulin-sensitizing properties. Evidence from in vitro studies has indicated that adiponectin has a potential role in reproduction because it reduces the production of androstenedione in bovine theca cells in vitro. However, this effect on androgen production has not yet been observed in vivo. The current study evaluated the effect of adiponectin on androstenedione secretion and oxidative stress parameters in a rodent model. Seven-week-old female Balb/c mice (n = 33), previously treated with equine gonadotropin chorionic, were assigned to one of four different treatments: Group 1, control (phosphate-buffered saline); Group 2, adiponectin 0.1 μg/mL; Group 3, adiponectin 1.0 μg/mL; Group 4, adiponectin 5.0 μg/mL. After 24 h, all animals were euthanized and androstenedione levels were measured in the serum while oxidative stress markers were quantified in whole ovary tissue. Female mice treated with adiponectin exhibited a significant reduction (about 60%) in serum androstenedione levels in comparison to controls. Androstenedione levels decreased from 0.78 ± 0.4 ng/mL (mean ± SD) in controls to 0.28 ± 0.06 ng/mL after adiponectin (5 μg/mL) treatment (P = 0.01). This change in androgen secretion after 24 hours of treatment was associated with a significant reduction in the expression of CYP11A1 and STAR (but not CYP17A1). In addition, ovarian AOPP product levels, a direct product of protein oxidation, decreased significantly in adiponectin-treated mice (5 μg/mL); AOPP (mean ± SD) decreased to 4.3 ± 2.1 μmol/L in comparison with that of the controls (11.5 ± 1.7 μmol/L; P = 0.0003). Our results demonstrated for the first time that acute treatment with adiponectin reduced the levels of a direct oxidative stress marker in the ovary as well as decreased androstenedione serum levels in vivo after 24 h.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: FVC KG. Performed the experiments: FVC AB RC MLR AMPD ASC GVB RNM. Analyzed the data: FVC. Contributed reagents/materials/analysis tools: PBDG RNM. Wrote the paper: FVC PBDG.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0154453