Human adenovirus species in children with acute respiratory illnesses
•This is a retrospective study of children with ARI whose HAdV-positive respiratory specimens were typed by specific qPCR assay.•The most common HAdV species detected were HAdV-B and HAdV-C•Patients with HAdV-C had higher odds of being admitted compared to those with HAdV-B.•No sufficient evidence o...
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| Published in: | Journal of clinical virology Vol. 134; p. 104716 |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Netherlands
Elsevier B.V
01-01-2021
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | •This is a retrospective study of children with ARI whose HAdV-positive respiratory specimens were typed by specific qPCR assay.•The most common HAdV species detected were HAdV-B and HAdV-C•Patients with HAdV-C had higher odds of being admitted compared to those with HAdV-B.•No sufficient evidence of an association between HAdV species and co-detection with other respiratory viruses were found.
Human adenovirus (HAdV) species B, C, and E are commonly associated with acute respiratory illnesses (ARI). We sought to determine the association between HAdV species and ARI severity in children over one respiratory season at Monroe Carell Jr. Children's Hospital at Vanderbilt.
We conducted a retrospective cohort study of children with HAdV from a provider-ordered BioFire® FilmArray Respiratory Pathogen Panel 2.0 (RPP) from 05/2018−06/2019. Type-specific PCR assays for HAdV-B3, B7, B11, B14, B16, B21, HAdV-C1, C2, C5, C6, and HAdV-E4 were performed. Demographics, clinical characteristics, and outcome data were compared between HAdV species.
Of 4514 respiratory specimens collected, 2644 (59 %) had at least one pathogen detected by RPP, and 384 (15 %) were HAdV-positive; 342 (89 %) were available for research testing with 306 (89 %) specimens from unique symptomatic individuals; 237 (77 %) were positive for the following species: 104 (44 %) HAdV-B, 114 (48 %) HAdV-C, 9 (4%) HAdV-E, and 10 (4%) with co-detection between species. The majority with identified HAdV species were seen in the ED (62 %), and approximately one-third were hospitalized. Patients with HAdV-C were more likely to be younger, hospitalized, and have a higher frequency of seizures compared to HAdV-B.
HAdV-C and HAdV-B were the most common species detected, with differences in clinical characteristics and outcomes noted. Additional studies with larger sample sizes focusing on a high-risk pediatric population are necessary to determine if differences in illness severity across individual HAdV types exist to guide further type-specific HAdV vaccine development. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 CRediT authorship contribution statement Varvara Probst: Conceptualization, Methodology, Formal analysis, Investigation, Writing - original draft, Visualization, Supervision, Project administration, Funding acquisition, Data curation. Emily K. Datyner: Methodology, Investigation, Writing - original draft. Zaid Haddadin: Writing - review & editing. Danielle A. Rankin: Formal analysis, Data curation, Writing - review & editing. Lubna Hamdan: Writing - review & editing. Herdi K Rahman: Formal analysis, Data curation, Visualization. Andrew Spieker: Formal analysis, Writing - review & editing. Laura S. Stewart: Resources, Writing - review & editing. Claudia Guevara: Investigation. Erin Yepsen: Investigation. Jonathan E. Schmitz: Conceptualization, Investigation, Writing - review & editing. Natasha B. Halasa: Supervision, Conceptualization, Methodology, Resources, Project administration, Funding acquisition, Writing - review & editing. |
| ISSN: | 1386-6532 1873-5967 |
| DOI: | 10.1016/j.jcv.2020.104716 |