Leptospira-rat-human relationship in Luzon, Philippines

Leptospira-rat-human relationship in Luzon, Philippines

Leptospirosis is a zoonotic infection that is caused by the pathogenic species of Leptospira. Rats are the most important reservoirs of these organisms. Our study aimed to characterize Leptospira isolates from humans and rats and elucidate the Leptospira-rat-human relationship in Luzon, Philippines....

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Bibliographic Details
Published in:Microbes and infection Vol. 16; no. 11; pp. 902 - 910
Main Authors: Villanueva, Sharon Yvette Angelina M., Saito, Mitsumasa, Baterna, Rubelia A., Estrada, Crystal Amiel M., Rivera, Ana Kriselda B., Dato, Micaella C., Zamora, Pia Regina Fatima C., Segawa, Takaya, Cavinta, Lolita L., Fukui, Takashi, Masuzawa, Toshiyuki, Yanagihara, Yasutake, Gloriani, Nina G., Yoshida, Shin-ichi
Format: Journal Article
Language:English
Published: France Elsevier Masson SAS 01-11-2014
Subjects:
Animals
Disease Models, Animal
Disease Reservoirs
Disease Transmission, Infectious
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Genotype
Geographic information system (GIS)
gyrB sequence analysis
Human
Humans
Leptospira
Leptospira interrogans
Leptospira interrogans - classification
Leptospira interrogans - genetics
Leptospira interrogans - immunology
Leptospira interrogans - isolation & purification
Leptospirosis - epidemiology
Leptospirosis - microbiology
Leptospirosis - transmission
Leptospirosis - veterinary
Male
Mesocricetus
Molecular Epidemiology
Molecular Sequence Data
Molecular Typing
Pathogenicity
PFGE
Philippines - epidemiology
Polymerase Chain Reaction
Rat
Rats
Rodent Diseases - epidemiology
Rodent Diseases - microbiology
Rodent Diseases - transmission
Sequence Analysis, DNA
Serogroup
Survival Analysis
Zoonoses - microbiology
Zoonoses - transmission
Philippines
Human
Pathogenicity
PFGE
Rat
Geographic information system (GIS)
gyrB sequence analysis
Leptospira
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Summary:Leptospirosis is a zoonotic infection that is caused by the pathogenic species of Leptospira. Rats are the most important reservoirs of these organisms. Our study aimed to characterize Leptospira isolates from humans and rats and elucidate the Leptospira-rat-human relationship in Luzon, Philippines. Forty strains were isolated from humans and rats. The isolates were confirmed to be Leptospira and pathogenic through rrl- and flaB-PCR, respectively. Around 73% of the isolates were found to be lethal to hamsters. Serotyping showed that there were mainly three predominant leptospiral serogroups in the study areas namely Pyrogenes, Bataviae, and Grippotyphosa. Gyrase B gene sequence analysis showed that all the isolates belonged to Leptospira interrogans. Most had 100% similarity with serovar Manilae (15/40), serovar Losbanos (8/40), and serogroup Grippotyphosa (8/40). Strains from each group had highly identical pulsed-field gel electrophoresis patterns and were further grouped as A (Pyrogenes, 14), B (Bataviae, 8), and C (Grippotyphosa, 10). Results further revealed that similar serotypes were isolated from both humans and rats in the same areas. It is suggested that these three predominant groups with highly similar intra-group PFGE patterns may have been primarily transmitted by rats and persistently caused leptospirosis in humans particularly in the Luzon islands.
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ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2014.07.001