Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions

Hydrogen sulfide is a highly toxic gas that causes many economic losses in aquaculture ponds. The application of sulfur-oxidizing bacteria (SOB) to remove hydrogen sulfide is an eco-friendly approach. This study aimed to isolate and identify the most efficient SOBs from the sediment of warm-water fi...

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Bibliographic Details
Published in:	Brazilian journal of microbiology Vol. 54; no. 4; pp. 3163 - 3172
Main Authors:	Dashtbin, Rana, Mahmoudi, Nemat, Besharati, Hossein, Lalevic, Blažo
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-12-2023 Springer Nature B.V
Subjects:	Aquaculture Aquariums Bacteria Bacteria - genetics Biomedical and Life Sciences Cell culture Cell morphology Culture media Cytology Economic impact Fish farms Fishponds Food Microbiology Hydrogen Hydrogen Sulfide Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Organic carbon Oxidation Oxidation-Reduction RNA, Ribosomal, 16S - genetics rRNA 16S Screening Soil and Agricultural Microbiology - Research Paper Strains (organisms) Sulfates Sulfur Sulfur oxidation Thiosulfate Thiosulfates Iran Biofertilizer Hydrogen sulfide Sustainable aquaculture Sulfur oxidizing bacteria fishpond Thiobacillus thioparus
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Summary:	Hydrogen sulfide is a highly toxic gas that causes many economic losses in aquaculture ponds. The application of sulfur-oxidizing bacteria (SOB) to remove hydrogen sulfide is an eco-friendly approach. This study aimed to isolate and identify the most efficient SOBs from the sediment of warm-water fish farms. Enrichment and isolation were performed in three different culture media (Starkey, Postgate, and H-3) based on both mineral and organic carbon. Overall, 27 isolates (14 autotrophic and 13 heterotrophic isolates) were purified based on colony and cell morphology differences. Initial screening was performed based on pH decrease. For final screening, the isolates were assessed based on their efficacy in thiosulfate oxidation and the sulfate production on Starkey liquid medium. Among isolated strains, 3 strains of Iran 2 (FH-13), Iran 3 (FH-21), and Iran 1 (FH-14) that belonged to Thiobacillus thioparus species (identified by 16s rRNA) showed the highest ability in thiosulfate oxidation (413.21, 1362.50, and 4188.03 mg/L for 14 days) and the highest sulfate production (3350, 2075, and 1600 mg/L). In the final phase, the performance of these strains under aquarium conditions showed that Iran 1 and Iran 2 had the highest ability in sulfur oxidation. In conclusion, Iran 1 and 2 strains can be used as effective SOB to remove hydrogen sulfide in fish farms. It is very important to evaluate strains in an appropriate strategy using a combination of different criteria to ensure optimal performance of SOB in farm conditions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1517-8382 1678-4405 1678-4405
DOI:	10.1007/s42770-023-01128-6