Increased Frequency of Genomic Alterations in Staphylococcus aureus during Chronic Infection Is in Part Due to Phage Mobilization

We assessed the nature and frequency of genome alterations in Staphylococcus aureus during chronic lung infection in patients with cystic fibrosis (CF) and during colonization of the nares in healthy individuals. Only individuals harboring the same S. aureus clone on consecutive samplings were inclu...

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Bibliographic Details
Published in:	The Journal of infectious diseases Vol. 189; no. 4; pp. 724 - 734
Main Authors:	Goerke, Christiane, y Papenberg, Saskia Matias, Dasbach, Simone, Dietz, Klaus, Ziebach, Rita, Kahl, Barbara C., Wolz, Christiane
Format:	Journal Article
Language:	English
Published:	Chicago, IL The University of Chicago Press 15-02-2004 University of Chicago Press Oxford University Press
Subjects:	Antibiotics Bacteria Bacterial Typing Techniques Bacteriology Bacteriophages Base Sequence Biological and medical sciences Cystic fibrosis Cystic Fibrosis - complications Cystic Fibrosis - microbiology DNA Primers Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Gels Genome, Bacterial Genomes Genomics Humans Infections Infectious diseases Medical sciences Microbiology Miscellaneous Mutation Open Reading Frames Pathogens Polymerase Chain Reaction Reference Values Restriction Mapping Sputum - microbiology Staphylococcal Infections - physiopathology Staphylococcal Infections - virology Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - genetics Staphylococcus aureus - virology Streptococcus Phages - genetics Streptococcus Phages - physiology Virology Virus Infection Chronic Microbiology Genomics Bacteriosis Bacteria Micrococcales Micrococcaceae Staphylococcal infection Staphylococcus aureus Phage
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Summary:	We assessed the nature and frequency of genome alterations in Staphylococcus aureus during chronic lung infection in patients with cystic fibrosis (CF) and during colonization of the nares in healthy individuals. Only individuals harboring the same S. aureus clone on consecutive samplings were included in the present study. Clone definition was based on pulsed-field gel electrophoresis (PFGE) analysis. Minor fragment variations in consecutive clones were interpreted as genome alterations. The frequency of genome alterations was significantly higher in S. aureus derived from patients with CF (mean time, 1.03 years) than in isolates derived from healthy individuals (mean time, 13.4 years). In total, 19 S. aureus strain pairs showing genome alterations were available for molecular analysis to clarify the nature of recombinational events in the host environment. In 8 cases, genome alteration could be linked to phage mobilization. Phage conversion of β-toxin production was evident in 7 pairs. In 1 strain pair, changes in the PFGE pattern were accompanied by deletion of a phage similar to ETA. Obviously, phage mobilization plays an important role in vivo. During long-termlung infection in patients with CF, the specific host response and/or the regular exposure to antibiotics exercises strong selective pressure on the pathogen. Genome plasticity may facilitate the adaptation to various host conditions.
Bibliography:	ark:/67375/HXZ-JTXXC3WN-X Financial support: Deutsche Forschungsgemeinschaft (grants Wo 578/3-2 and Wo 578/3-3); Mukoviszidose e.V. istex:E196248987F5B967A570E92BE6172D0DE59A94E9 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1899 1537-6613
DOI:	10.1086/381502