Search Results - "Das, Anuska"

Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA by Goswami, Hemant N, Rai, Jay, Das, Anuska, Li, Hong

“…Cas7-11 is a Type III-E CRISPR Cas effector that confers programmable RNA cleavage and has potential applications in RNA interference. Cas7-11 encodes a single…”
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The molecular basis for recognition of 5′-NNNCC-3′ PAM and its methylation state by Acidothermus cellulolyticus Cas9 by Das, Anuska, Hand, Travis H., Smith, Chardasia L., Wickline, Ethan, Zawrotny, Michael, Li, Hong

“…Acidothermus cellulolyticus CRISPR-Cas9 (AceCas9) is a thermophilic Type II-C enzyme that has potential genome editing applications in extreme environments. It…”
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Structural and Biochemical Analysis of a Methylation Sensitive Cas9 by Das, Anuska

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Directed evolution studies of a thermophilic Type II-C Cas9 by Hand, Travis H, Das, Anuska, Li, Hong

“…Though making up nearly half of the known CRISPR-Cas9 family of enzymes, the Type II-C CRISPR-Cas9 has been underexplored for their molecular mechanisms and…”
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Structural principles of CRISPR-Cas enzymes used in nucleic acid detection by Das, Anuska, Goswami, Hemant N., Whyms, Charlisa T., Sridhara, Sagar, Li, Hong

“…[Display omitted] •Three types CRISPR-Cas systems including the associated ancillary enzymes are used in nucleic acid detections.•Molecular mechanisms of these…”
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Structural and Biochemical Characterization of a NovelMethylation Sensitive Cas9 by Das, Anuska

“…Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) – Cas9 system is an RNA guided DNA cleavage mechanism that has been repurposed for many…”
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Solvent Accessibility of CRISPR-CAS9 Target DNA is Correlated with Substrate Specificity by Hand, Travis H., Das, Anuska, Duboy, Emily, Roth, Mitchell, Smith, Chardasia, Baptist, Uriel, Li, Hong

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Coupled catalytic states and the role of metal coordination in Cas9 by Das, Anuska, Rai, Jay, Roth, Mitchell O., Shu, Yuerong, Medina, Megan L., Barakat, Mackenzie R., Li, Hong

“…Controlling the activity of the CRISPR–Cas9 system is essential to its safe adoption for clinical and research applications. Although the conformational…”
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Phosphate Lock Residues of Acidothermus cellulolyticus Cas9 Are Critical to Its Substrate Specificity by Hand, Travis H, Das, Anuska, Roth, Mitchell O, Smith, Chardasia L, Jean-Baptiste, Uriel L, Li, Hong

“…Despite being utilized widely in genome sciences, CRISPR-Cas9 remains limited in achieving high fidelity in cleaving DNA. A better understanding of the…”
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