Search Results - "Darzynkiewicz, E"

Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo by Kuhn, A N, Diken, M, Kreiter, S, Selmi, A, Kowalska, J, Jemielity, J, Darzynkiewicz, E, Huber, C, Türeci, Ö, Sahin, U

“…Vaccination with in vitro transcribed RNA coding for tumor antigens is considered a promising approach for cancer immunotherapy and has already entered human…”
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Weak binding affinity of human 4EHP for mRNA cap analogs by Zuberek, Joanna, Kubacka, Dorota, Jablonowska, Agnieszka, Jemielity, Jacek, Stepinski, Janusz, Sonenberg, Nahum, Darzynkiewicz, Edward

“…Ribosome recruitment to the majority of eukaryotic mRNAs is facilitated by the interaction of the cap binding protein, eIF4E, with the mRNA 5' cap structure…”
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Stopped-flow and Brownian dynamics studies of electrostatic effects in the kinetics of binding of 7-methyl-GpppG to the protein eIF4E by Błachut-Okrasińska, E, Bojarska, E, Niedźwiecka, A, Chlebicka, L, Darzynkiewicz, E, Stolarski, R, St piński, J, Antosiewicz, J M

“…The kinetics of binding 7-methyl-GpppG, an analogue of the 5'-mRNA cap, to the cap-binding protein eIF4E, at 20 degrees C, in 50 mM Hepes-KOH buffer, pH 7.2,…”
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Effects of pH on kinetics of binding of mRNA-cap analogs by translation initiation factor eIF4E by Długosz, M, Błachut-Okrasińska, E, Bojarska, E, Darzynkiewicz, E, Antosiewicz, J M

“…Stopped-flow spectrofluorimetry and a theoretical method for predicting protonation equilibria in polyelectrolytes were combined in an analysis of the pH…”
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Kinetics of C. Elegans DcpS Cap Hydrolysis Studied by Fluorescence Spectroscopy by Wierzchowski, J., Pietrzak, M., Stepinski, J., Jemielity, J., Kalek, M., Bojarska, E., Jankowska-Anyszka, M., Davis, R. E., Darzynkiewicz, E.

“…DcpS (scavenger decapping enzyme) from nematode C. elegans readily hydrolyzes both monomethyl- and trimethylguanosine cap analogues. The reaction was followed…”
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Synthesis and properties of mRNAs containing the novel “anti-reverse” cap analogs 7-methyl(3′-O-methyl)GpppG and 7-methyl(3′-deoxy)GpppG by STEPINSKI, JANUSZ, WADDELL, CLINT, STOLARSKI, RYSZARD, DARZYNKIEWICZ, EDWARD, RHOADS, ROBERT E.

“…The ability to synthesize capped RNA transcripts in vitro using bacteriophage polymerases has been of considerable value in a variety of applications. However,…”
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Purine nucleoside phosphorylase. Structure-activity relationships for substrate and inhibitor properties of N-1-, N-7-, and C-8-substituted analogues; differentiation of mammalian and bacterial enzymes with N-1-methylinosine and guanosine by Bzowska, A, Kulikowska, E, Darzynkiewicz, E, Shugar, D

“…The previous finding that 7-methylinosine (m7Ino) and 7-methylguanosine (m7Guo) are excellent, as well as fluorescent, substrates for calf spleen purine…”
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Molecular recognition of mRNA 5′ cap by 3′ poly(A)-specific ribonuclease (PARN) differs from interactions known for other cap-binding proteins by Niedzwiecka, Anna, Nilsson, Per, Worch, Remigiusz, Stepinski, Janusz, Darzynkiewicz, Edward, Virtanen, Anders

“…The mRNA 5′ cap structure plays a pivotal role in coordination of eukaryotic translation and mRNA degradation. Poly(A)-specific ribonuclease (PARN) is a…”
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Novel "anti-reverse" cap analogs with superior translational properties by Jemielity, Jacek, Fowler, Tolvert, Zuberek, Joanna, Stepinski, Janusz, Lewdorowicz, Magdalena, Niedzwiecka, Anna, Stolarski, Ryszard, Darzynkiewicz, Edward, Rhoads, Robert E

“…Synthetic analogs of the 5'-terminal caps of eukaryotic mRNAs and snRNAs are used in elucidating such physiological processes as mRNA translation, pre-mRNA…”
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Biophysical Studies of eIF4E Cap-binding Protein: Recognition of mRNA 5′ Cap Structure and Synthetic Fragments of eIF4G and 4E-BP1 Proteins by Niedzwiecka, Anna, Marcotrigiano, Joseph, Stepinski, Janusz, Jankowska-Anyszka, Marzena, Wyslouch-Cieszynska, Aleksandra, Dadlez, Michal, Gingras, Anne-Claude, Mak, Pawel, Darzynkiewicz, Edward, Sonenberg, Nahum, Burley, Stephen K., Stolarski, Ryszard

“…mRNA 5′-cap recognition by the eukaryotic translation initiation factor eIF4E has been exhaustively characterized with the aid of a novel fluorometric,…”
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mRNAs containing the histone 3' stem-loop are degraded primarily by decapping mediated by oligouridylation of the 3' end by Su, Wei, Slepenkov, Sergey V, Slevin, Michael K, Lyons, Shawn M, Ziemniak, Marcin, Kowalska, Joanna, Darzynkiewicz, Edward, Jemielity, Jacek, Marzluff, William F, Rhoads, Robert E

“…Metazoan replication-dependent histone mRNAs are only present in S-phase, due partly to changes in their stability. These mRNAs end in a unique stem-loop (SL)…”
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Synthesis and evaluation of stability of m 3 G-CAP analogues in serum-supplemented medium and cytosolic extract by Honcharenko, M, Zytek, M, Bestas, B, Moreno, P, Jemielity, J, Darzynkiewicz, E, Smith, CIE, Stromberg, R

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Differential Inhibition of mRNA Degradation Pathways by Novel Cap Analogs by Grudzien, Ewa, Kalek, Marcin, Jemielity, Jacek, Darzynkiewicz, Edward, Rhoads, Robert E.

“…mRNA degradation predominantly proceeds through two alternative routes: the 5′→3′ pathway, which requires deadenylation followed by decapping and 5′→3′…”
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Synthesis and evaluation of stability of m3G-CAP analogues in serum-supplemented medium and cytosolic extract by Honcharenko, Malgorzata, Zytek, Malgorzata, Bestas, Burcu, Moreno, Pedro, Jemielity, Jacek, Darzynkiewicz, Edward, Smith, C.I. Edvard, Strömberg, Roger

“…Increased efficiency in splice-correction (splice-switching) has been shown by use of a synthetic RNA 5′-end nuclear localization signal composed of an…”
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Novel cap analogs for in vitro synthesis of mRNAs with high translational efficiency by Grudzien, Ewa, Stepinski, Janusz, Jankowska-Anyszka, Marzena, Stolarski, Ryszard, Darzynkiewicz, Edward, Rhoads, Robert E

“…Synthetic analogs of the N7-methylated guanosine triphosphate cap at the 5' end of eukaryotic mRNAs and snRNAs have played an important role in understanding…”
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Thermodynamics of mRNA 5‘ Cap Binding by Eukaryotic Translation Initiation Factor eIF4E by Niedzwiecka, Anna, Darzynkiewicz, Edward, Stolarski, Ryszard

“…Translation of mRNA in eukaryotes begins with specific recognition of the 5‘ cap structure by the highly conserved protein, eIF4E. The thermodynamics of eIF4E…”
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Influence of Electric Charge Variation at Residues 209 and 159 on the Interaction of eIF4E with the mRNA 5‘ Terminus by Zuberek, Joanna, Jemielity, Jacek, Jablonowska, Agnieszka, Stepinski, Janusz, Dadlez, Michał, Stolarski, Ryszard, Darzynkiewicz, Edward

“…Eukaryotic translation initiation factor 4E (eIF4E) is essential for efficient protein synthesis in cap-dependent translation. The protein specifically binds…”
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Specificity of recognition of mRNA 5' cap by human nuclear cap-binding complex by Worch, Remigiusz, Niedzwiecka, Anna, Stepinski, Janusz, Mazza, Catherine, Jankowska-Anyszka, Marzena, Darzynkiewicz, Edward, Cusack, Stephen, Stolarski, Ryszard

“…The heterodimeric nuclear cap-binding complex (CBC) binds to the mono-methylated 5' cap of eukaryotic RNA polymerase II transcripts such as mRNA and U snRNA…”
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A direct method for the synthesis of nucleoside 5′-methylenebis(phosphonate)s from nucleosides by Kalek, Marcin, Jemielity, Jacek, Stepinski, Janusz, Stolarski, Ryszard, Darzynkiewicz, Edward

“…[Display omitted] An efficient method for the preparation of nucleoside 5′-methylenebis(phosphonate)s has been developed. Unprotected nucleosides are…”
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Enzymatically stable 5′ mRNA cap analogs: Synthesis and binding studies with human DcpS decapping enzyme by Kalek, Marcin, Jemielity, Jacek, Darzynkiewicz, Zbigniew M., Bojarska, Elzbieta, Stepinski, Janusz, Stolarski, Ryszard, Davis, Richard E., Darzynkiewicz, Edward

“…Four novel 5′ mRNA cap analogs have been synthesized with one of the pyrophosphate bridge oxygen atoms of the triphosphate linkage replaced with a methylene…”
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