Expression of miR-29, miR-125, and miR-181 in the anterior kidneys of Streptococcus-infected Nile tilapia (Oreochromis niloticus)

[Abstract] MicroRNAs (miRNAs) are a group of small non-coding RNAs which posttranscriptionally regulate gene expression in eukaryotic cells. In this study, three miRNAs participating in the immune function in mammalian cells: miR-29, miR-125, and miR-181 have been characterized in Nile tilapia (Oreo...

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Bibliographic Details
Published in:	The Japanese Journal of Veterinary Research Vol. 66; no. 3; pp. 165 - 176
Main Authors:	Danudej Chiachanpongse, Atthaporn Roongsitthichai, Win Surachetpong
Format:	Journal Article
Language:	English
Published:	Faculty of Veterinary Medicine, Hokkaido University 01-08-2018
Online Access:	Get full text
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Summary:	[Abstract] MicroRNAs (miRNAs) are a group of small non-coding RNAs which posttranscriptionally regulate gene expression in eukaryotic cells. In this study, three miRNAs participating in the immune function in mammalian cells: miR-29, miR-125, and miR-181 have been characterized in Nile tilapia (Oreochromis niloticus). A comparison of all three miRNAs in Nile tilapia and other organisms, including zebrafish, mouse, rat, chicken, pig, and human revealed identical sequence of mature miRNA sequences. The genomic sequences of miR-29, miR-125, and miR-181 of Nile tilapia have been amplified using polymerase chain reaction (PCR) and cloned into pGT-19T plasmid. The plasmids containing the sequences of miR-29, miR-125, and miR-181 were used for standardization and validation of quantitative PCR (qPCR) methods. Moreover, the qPCR methods have been applied as a tool to determine the expression of all three miRNAs in the anterior kidneys of Streptococcus agalactiae-infected tilapia. Specifically, expression of miR-29, miR-125, and miR-181 reduced significantly at 3 days post bacterial challenge. In silico analysis of three miRNA targets revealed that multiple genes could be regulated by these miRNAs including Ifi30, Cd276, Lfng, Cdc42se1, Ddx3x, Il1a, and Atg5. In conclusion, these results demonstrated the expression level and molecular targets of miRNAs in Nile tilapia which could be applied for the understanding of these miRNAs during pathogen-host interaction.
ISSN:	0047-1917
DOI:	10.14943/jjvr.66.3.165