Characterization and Validation Studies of Powerplex™ 2.1, A Nine-locus Short Tandem Repeat (Str) Multiplex System and Penta D Monoplex

In order to increase the power of discrimination for human identification purposes, a nine-locus short tandem repeat (STR) multiplex, the GenePrint® PowerPlex™ 2.1 system (PowerPlex™ 2.1) developed by Promega Corporation and a separate pentanucleotide-repeat locus, Penta D, were tested. This megaple...

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Bibliographic Details
Published in:	Journal of forensic sciences Vol. 47; no. 4; pp. 1 - 16
Main Authors:	Levedakou, EN, Freeman, DA, Budzynski, MJ, Early, BE, Damaso, RC, Pollard, AM, Townley, AJ, Gombos, JL, Lewis, JL, Kist, FG, Hockensmith, ME, Terwilliger, ML, Amiott, E, McElfresh, KC, Schumm, JW, Ulery, SR, Konotop, F, Sessa, TL, Sailus, JS, Crouse, CA, Tomsey, CS, Ban, JD, Nelson, MS
Format:	Journal Article
Language:	English
Published:	United States 01-07-2002
Subjects:	Animals Bacteria DNA Fingerprinting - methods DNA Primers Forensic Medicine - methods Humans Observer Variation Polymerase Chain Reaction Primates Reproducibility of Results Sensitivity and Specificity Tandem Repeat Sequences - genetics vWA D7S820 TPOX primer sequences D5S818 forensic science TH01 PowerPlex™ 1.1 PowerPlex™ 2.1 Penta D allele microvariants Penta E CSF1PO polymerase chain reaction D3S1358 DNA typing FGA short tandem repeats D21S11 forensics D18S51 multiplex D16S539 D13S317 D8S1179
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Summary:	In order to increase the power of discrimination for human identification purposes, a nine-locus short tandem repeat (STR) multiplex, the GenePrint® PowerPlex™ 2.1 system (PowerPlex™ 2.1) developed by Promega Corporation and a separate pentanucleotide-repeat locus, Penta D, were tested. This megaplex system includes the highly polymorphic loci FGA, TPOX, D8S1179, vWA, Penta E, D18S51, D21S11, TH01, and D3S1358 and may be used in combination with the eight-locus STR multiplex, the GenePrint® PowerPlex™ 1.1 system (PowerPlex™ 1.1) that has been previously developed. Three of the loci, TPOX, TH01 and vWA, have been included in both systems for quality control purposes. As with PowerPlex™ 1.1, PowerPlex™ 2.1 is also based on a two-color detection of fluorescent-labeled DNA products amplified by polymerase chain reaction (PCR) and provides a valuable tool for accurate and rapid allele determination. The primer sequences used in the PowerPlex™ 2.1/Penta D system are also presented in this report. To meet the "Quality Assurance Standards for Forensic DNA Testing Laboratories" (FBI), we tested the efficiency and reproducibility of the PowerPlex™ 2.1/Penta D system by several validation studies that were conducted as a joint project among seven laboratories. Validation tests included concordance studies, sensitivity, and species specificity determination, as well as performance in forensic and environmentally impacted samples. The results produced from these tests demonstrated the consistency and reliability of the PowerPlex™ 2.1/Penta D system.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1198 1556-4029
DOI:	10.1520/JFS15443J