Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro

Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro

Contents We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and there...

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Published in:Reproduction in domestic animals Vol. 52; no. 3; pp. 409 - 421
Main Authors: Ambrogi, M, Dall'Acqua, PC, Rocha‐Frigoni, NAS, Leão, BCS, Mingoti, GZ
Format: Journal Article
Language:English
Published: Germany Blackwell Publishing Ltd 01-06-2017
Subjects:
Animals
Antioxidants
Antioxidants - pharmacology
Blastocysts
Bovine serum albumin
Catalase
Catalase - pharmacology
Cattle
Cold tolerance
Culture Media
Cysteamine
Cysteamine - pharmacology
Cysteine
Cysteine - pharmacology
Cytoplasm - physiology
Embryo Culture Techniques - veterinary
Embryogenesis
Embryonic growth stage
Embryos
In Vitro Oocyte Maturation Techniques - methods
In Vitro Oocyte Maturation Techniques - veterinary
In vitro testing
Macromolecules
Maturation
Membrane Potential, Mitochondrial
Mitochondria
Oocytes
Reactive Oxygen Species
Serum albumin
Transport
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Summary:Contents We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p < .05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS: 1.00, p < .05). After IVM, the mitochondrial membrane potentials of the transported oocytes were lower than those of the non‐transported oocytes (FCS: 0.41 and BSA: 0.57 vs. Control FCS: 1.00, p < .05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS: 1.00 and Control Antiox: 0.84, p < .05). The blastocyst formation rates (36.9% average) and the re‐expansion rates of vitrified‐warmed blastocysts (53%, average) were unaffected (p > .05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance.
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ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12923