The effects of preoperative immunosuppressive therapy on ischemia and reperfusion (I/R) injury in healthy rats

Purpose Warm-ischemia-induced injuries might be encountered during renal transplants from cadavers and healthy donors. Toll-like receptors (TLR) in ischemia–reperfusion (I/R) injury are one of the indicators of intracellular injury pathways. The intensity of ischemic injury is directly proportionate...

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Published in:	International urology and nephrology Vol. 46; no. 2; pp. 389 - 393
Main Authors:	Kilinc, Selcuk, Tan, Sedat, Kolatan, Efsun Hatice, Ruscuklu, Dane, Satici, Esra, Kemiksiz, Mehmet, Dalkilic, Lutfi, Erdogdu, Umut Eren, Karaca, Cezmi
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-02-2014 Springer Nature B.V
Subjects:	Animals Gene Expression - drug effects Immunosuppressive Agents - pharmacology Immunosuppressive Agents - therapeutic use Kidney Transplantation Medicine Medicine & Public Health Nephrology Preoperative Care Rats Rats, Wistar Reperfusion Injury - blood Reperfusion Injury - prevention & control RNA - blood Tacrolimus - pharmacology Tacrolimus - therapeutic use Toll-Like Receptor 2 - genetics Toll-Like Receptor 4 - genetics Urology Urology - Original Paper Toll-like receptors Rat model Ischemia–reperfusion injury TLR
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Summary:	Purpose Warm-ischemia-induced injuries might be encountered during renal transplants from cadavers and healthy donors. Toll-like receptors (TLR) in ischemia–reperfusion (I/R) injury are one of the indicators of intracellular injury pathways. The intensity of ischemic injury is directly proportionate to high TLR levels. To minimize the I/R injury, we investigated TLR2 and TLR4 levels on rats, which were pretreated with tacrolimus (FK506) before I/R. Methods Eight Wistar albino rats in the study group were administered .01 mg/kg intramuscular tacrolimus. Administration to the study group was performed 24 and 1 h before warm ischemia. Eight rats in the control group were injected with 0.1 c.c. of distilled water. Blood samples were collected from the tail veins of all the rats on the first, second and third days. Expression levels of TLR2 and TLR4 genes were analyzed using the polymerase chain reaction method, to determine any significant difference between the control and study groups on the days when blood was taken. Results TLR2 ( p = 0.045) and TLR4 ( p = 0.022) levels in the study group were found to be statistically, and significantly, lower than those in the control group, on the second day following warm-ischemia- and reperfusion-induced injury. Conclusions Administration of immunosuppressive drugs to healthy donor rats led to a statistically significant reduction in the expression levels of TLR2 and TLR4 in the early period. In light of the data obtained by this study, we hypothesize that a preoperative therapy on donors might have a role in preventing I/R injury.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0301-1623 1573-2584
DOI:	10.1007/s11255-013-0548-2