In vitro activity of antifungals in combination with essential oils against the oomycete Pythium insidiosum

In vitro activity of antifungals in combination with essential oils against the oomycete Pythium insidiosum

Aims The aim of this study was to investigate the in vitro susceptibility of Pythium insidiosum to combinations of the antifungal drugs terbinafine or itraconazole with Melaleuca alternifolia, Mentha piperita and Origanum vulgare essential oils (EOs). Methods and Results In vitro combinations of ant...

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Published in:Journal of applied microbiology Vol. 121; no. 4; pp. 998 - 1003
Main Authors: Valente, J.S., Fonseca, A.O.S., Denardi, L.B., Dal Ben, V.S., Maia Filho, F.S., Zambrano, C.G., Braga, C.Q., Alves, S.H., Botton, S.A., Brayer Pereira, D.I.
Format: Journal Article
Language:English
Published: England Oxford University Press 01-10-2016
Subjects:
Animals
Anti-Infective Agents - pharmacology
Antifungal Agents - pharmacology
azoles
Brazil
Drug Therapy, Combination
Fungal infections
Horse Diseases - drug therapy
Horses
Melaleuca alternifolia
Mentha piperita
Mentha piperita - chemistry
Microbial Sensitivity Tests
Microbiology
Naphthalenes - pharmacology
Oils & fats
Oils, Volatile - pharmacology
Oomycetes
Origanum - chemistry
Origanum vulgare
pythiosis
Pythiosis - drug therapy
Pythiosis - parasitology
Pythium - drug effects
Pythium insidiosum
terbinafine
Mentha piperita
terbinafine
azoles
Origanum vulgare
Melaleuca alternifolia
pythiosis
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Summary:Aims The aim of this study was to investigate the in vitro susceptibility of Pythium insidiosum to combinations of the antifungal drugs terbinafine or itraconazole with Melaleuca alternifolia, Mentha piperita and Origanum vulgare essential oils (EOs). Methods and Results In vitro combinations of antifungal drugs with EOs were evaluated by checkerboard microdilution method against 20 Brazilian isolates of P. insidiosum. The tests were performed according to protocol M38‐A2, and the interpretation of each combination result was based on the values of the fractional inhibitory concentration index. The combinations of itraconazole with EOs presented prominent synergistic effects against P. insidiosum isolates, and no antagonism was observed with these combinations. However, the combinations of terbinafine with EOs resulted in indifferent or antagonistic effects. Conclusions The combination of plant‐derived bioactive compounds with antifungal drugs may be an alternative therapy for the control of infections caused by P. insidiosum. Studies of new therapeutic protocols involving these proposed combinations are needed. Significance and Impact of the Study The antimicrobial combinations using EOs with terbinafine or itraconazole can be an attractive therapeutic option for controlling P. insidiosum infections.
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ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13234