Bialaphos selection of stable transformants from maize cell culture

Stable transformed Black Mexican Sweet (BMS) maize callus was recovered from suspension culture cells bombarded with plasmid DNA that conferred resistance to the herbicide bialaphos. Suspension culture cells were bombarded with a mixture of two plasmids. One plasmid contained a selectable marker gen...

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Bibliographic Details
Published in:	Theoretical and applied genetics Vol. 79; no. 5; pp. 625 - 631
Main Authors:	Spencer, T.M, Gordon-Kamm, W.J, Daines, R.J, Start, W.G, Lemaux, P.G
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-05-1990 Berlin
Subjects:	Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions artificial selection bar gene beta-glucuronidase Biological and medical sciences callus Classical genetics, quantitative genetics, hybrids enzymes Fundamental and applied biological sciences. Psychology genes genetic markers genetic transformation Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution glucuronidase herbicides microprojectile bombardment phosphinothricin acetyltransferase Pteridophyta, spermatophyta resistance tissue culture Varietal selection. Specialized plant breeding, plant breeding aims Vegetals Zea mays Monocotyledones Callus Zea mays Genetic marker Enzyme β-D-Glucuronidase Artificial selection Cell transformation Herbicide Cereal crop Resistance Suspension culture Enzymatic activity Gramineae Angiospermae Spermatophyta Southern blotting
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Summary:	Stable transformed Black Mexican Sweet (BMS) maize callus was recovered from suspension culture cells bombarded with plasmid DNA that conferred resistance to the herbicide bialaphos. Suspension culture cells were bombarded with a mixture of two plasmids. One plasmid contained a selectable marker gene, bar, which encoded phosphinothricin acetyl transferase (PAT), and the other plasmid encoded a screenable marker for β-glucuronidase (GUS). Bombarded cells were selected on medium containing the herbicide bialaphos, which is cleaved in plant cells to yield phosphinothricin (PPT), an inhibitor of glutamine synthetase. The bialaphos-resistant callus contained the bar gene and expressed PAT as assayed by PPT inactivation. Transformants that expressed high levels of PAT grew more rapidly on increasing concentrations of bialaphos than transformants expressing low levels of PAT. Fifty percent of the bialaphos-resistant transformants tested (8 of 16) expressed the nonselected gene encoding GUS.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0040-5752 1432-2242
DOI:	10.1007/BF00226875