Coagulation assays based on the Luminescent Oxygen Channeling Immunoassay technology 1

The Luminescent Oxygen Channeling Immunoassay (LOCI) technology is a well-established homogeneous assay format that allows for fast, accurate, and highly sensitive quantitation of analytes. We set out to develop and prove a novel concept to establish a LOCI format that should principally allow for t...

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Bibliographic Details
Published in:	Clinical chemistry and laboratory medicine Vol. 49; no. 5; p. 855
Main Authors:	Kappel, Andreas, Stephan, Sina, Christ, Gerlinde, Haude-Barten, Anja, Dahm, Madeleine, Schwarz, Herbert, Fischer, Bodo, Hahn, Martin, Althaus, Harald, Ehm, Matthias, Vitzthum, Frank
Format:	Journal Article
Language:	English
Published:	Germany 01-05-2011
Subjects:	Antithrombins - blood Antithrombins - pharmacology Blood Coagulation Tests - methods Humans Immunoassay - methods Luminescent Measurements - methods Singlet Oxygen - chemistry
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Summary:	The Luminescent Oxygen Channeling Immunoassay (LOCI) technology is a well-established homogeneous assay format that allows for fast, accurate, and highly sensitive quantitation of analytes. We set out to develop and prove a novel concept to establish a LOCI format that should principally allow for the determination of the activity of coagulation factors and anticoagulants of clinical relevance. The concept is based on the linkage of LOCI nano-beads by a peptide that can be cleaved by a coagulation factor. To prove the principle, we used a peptide that can be cleaved by thrombin. We were able to show that coagulation activation of plasma or whole blood samples that were combined with the LOCI components degraded the thrombin-sensitive peptide and consequently, led to a reduction of the LOCI signal. Signal reduction was proportional to the amount of active thrombin generated. The research prototype assay allowed for the detection of factor deficiencies in both the extrinsic and intrinsic coagulation pathways, and for the quantification of hirudin, a direct thrombin inhibitor. Taken together, we conclude that the LOCI technology has the potential for extension to functional blood coagulation assays.
ISSN:	1437-4331
DOI:	10.1515/CCLM.2011.132