Virucidal Efficacy of Laser-Generated Copper Nanoparticle Coatings Against Model Coronavirus and Herpesvirus
High-efficiency antiviral surfaces can be effective means to fight against viral diseases such as the recent Covid-19 pandemic. Copper and copper oxides, as well as their nanoparticles (Cu NPs) and coatings, are among the effective antiviral materials having internal and external biocidal effects on...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
28-07-2023
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | High-efficiency antiviral surfaces can be effective means to fight against
viral diseases such as the recent Covid-19 pandemic. Copper and copper oxides,
as well as their nanoparticles (Cu NPs) and coatings, are among the effective
antiviral materials having internal and external biocidal effects on viruses.
In this work, stable Cu NP colloids were produced via femtosecond laser
ablation of the metal target in water containing sodium citrate. Raman
spectroscopy and X-ray diffraction studies confirmed that the 32 nm mean size
nanoparticles are mixtures of mainly metallic copper and copper (I) oxide Cu2O.
Polyvinyl butyral was utilized as the binding agent for the spray-coated Cu
NPs. The virucidal efficacy of such coatings containing different Cu content
ranging from 2.9 at.% to 11.2 at.%. was confirmed against animal-origin
coronavirus containing RNA, the agent of avian infectious bronchitis (IBV), and
herpesvirus containing DNA, the agent of bovine herpesvirus (BoHV-1) infection.
It was demonstrated that after a short time of exposure, the Cu NPs-based
coatings do not have a toxic effect on the cell cultures while demonstrating a
negative effect on the biological activity of both model viruses that was
confirmed by quantification of the viruses via the determination of tissue
culture infectious dose (TCID50) virus titre and their viral nucleic acids via
determination of threshold cycle (Ct) employing real-time polymerase chain
reaction analysis. The assays showed that the decrease in TCID50 virus titre
and increase in Ct values correlated with Cu content in Cu NPs-based coatings
for both investigated viruses. Contact with coatings decreased IBV and BoHV-1
numbers from 99.42% to 100.00% and from 98.65% to 99.96% respectively. These
findings suggest that Cu NPs show inhibitory effects leading to the
inactivation of viruses and their nuclei regardless of the presence of a viral
envelope. |
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| DOI: | 10.48550/arxiv.2308.02404 |