Bioactive cell-like hybrids coassembled from (glyco)dendrimersomes with bacterial membranes

A library of amphiphilic Janus dendrimers including two that are fluorescent and one glycodendrimer presenting lactose were used to construct giant dendrimersomes and glycodendrimersomes. Coassembly with the components of bacterial membrane vesicles by a dehydration–rehydration process generated gia...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 113; no. 9; pp. E1134 - E1141
Main Authors:	Xiao, Qi, Yadavalli, Srujana S., Zhang, Shaodong, Sherman, Samuel E., Fiorin, Elodie, da Silva, Louise, Wilson, Daniela A., Hammer, Daniel A., André, Sabine, Gabius, Hans-Joachim, Klein, Michael L., Goulian, Mark, Percec, Virgil
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 01-03-2016 National Acad Sciences
Series:	PNAS Plus
Subjects:	Bacteria Biological activity Biological Sciences Cell Wall - metabolism Cells Dendrimers - metabolism Escherichia coli Escherichia coli - metabolism Fluorescence Janus Membranes Physical Sciences PNAS Plus Proteins lipopolysaccharides transmembrane protein E. coli galectin
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Summary:	A library of amphiphilic Janus dendrimers including two that are fluorescent and one glycodendrimer presenting lactose were used to construct giant dendrimersomes and glycodendrimersomes. Coassembly with the components of bacterial membrane vesicles by a dehydration–rehydration process generated giant cell-like hybrid vesicles, whereas the injection of their ethanol solution into PBS produced monodisperse nanometer size assemblies. These hybrid vesicles contain transmembrane proteins including a small membrane protein, MgrB, tagged with a red fluorescent protein, lipopolysaccharides, and glycoproteins from the bacterium Escherichia coli. Incorporation of two colored fluorescent probes in each of the components allowed fluorescence microscopy to visualize and demonstrate coassembly and the incorporation of functional membrane channels. Importantly, the hybrid vesicles bind a human galectin, consistent with the display of sugar moieties from lipopolysaccharides or possibly glycosylated membrane proteins. The present coassembly method is likely to create cell-like hybrids from any biological membrane including human cells and thus may enable practical application in nanomedicine.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewers: L.P., Centre Interdisciplinaire de Nanoscience de Marseille (CINaM) CNRS UMR 7325; and D.T., NanoSynthons LLC. 2Present address: Institute for Molecules and Materials, Radboud University Nijmegen, 6525 AJ, Nijmegen, The Netherlands. Contributed by Michael L. Klein, January 22, 2016 (sent for review December 26, 2015; reviewed by Ling Peng and Donald Tomalia) Author contributions: Q.X., S.S.Y., S.Z., M.G., and V.P. designed research; Q.X., S.S.Y., S.Z., and S.E.S. performed research; E.F., L.d.S., D.A.W., D.A.H., S.A., and H.-J.G. contributed new reagents/analytic tools; Q.X., S.S.Y., S.Z., M.G., and V.P. analyzed data; and Q.X., S.S.Y., S.E.S., H.-J.G., M.L.K., M.G., and V.P. wrote the paper. 1Q.X. and S.S.Y. contributed equally to this work.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1525589113