Determination of Disulfiram by Micellar Liquid Chromatography in Illicit Preparations

Determination of Disulfiram by Micellar Liquid Chromatography in Illicit Preparations

Presently, disulfiram is used in aversion therapy for recovering alcoholics. It acts by inhibiting aldehyde dehydrogenase, leading to high blood levels of acetaldehyde. A simple direct injection micellar liquid chromatographic procedure was developed to determine disulfiram in illicit preparations (...

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Bibliographic Details
Published in:Journal of AOAC International Vol. 94; no. 4; pp. 1082 - 1088
Main Authors: MOURVA, Sandeep-Kumar, DUBEV, Swati, DURGABANSHI, Abhilasha, SUDHEER KUMAR SHUKLA, ESTEVE-ROMERO, Joseph, BOSE, Devasish
Format: Journal Article
Language:English
Published: Gaithersburg, MD AOAC International 01-07-2011
Oxford University Press
Subjects:
Alcoholics
Aversion therapy
Biological and medical sciences
Biological Products - chemistry
Care and treatment
Chemical properties
Chromatography, Liquid - methods
Disulfiram
Disulfiram - chemistry
Food industries
Fundamental and applied biological sciences. Psychology
Humans
Identification and classification
Liquid chromatography
Methods
Molecular Structure
Reproducibility of Results
Street Drugs - chemistry
Urine - chemistry
India
Disulfiram
Preparation
Liquid chromatography
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Summary:Presently, disulfiram is used in aversion therapy for recovering alcoholics. It acts by inhibiting aldehyde dehydrogenase, leading to high blood levels of acetaldehyde. A simple direct injection micellar liquid chromatographic procedure was developed to determine disulfiram in illicit preparations (ayurvedic, herbal, divine ash, and traditional medicine), as well as in pharmaceuticals and biological samples (urine). After application of a predictive optimization strategy, the proposed method was developed using a 0.1 M sodium dodecyl sulfate-butanol 4% (v/v) buffered to pH 7 as the mobile phase at a flow rate of 1 mL/min, an octyl silyl (C8) 150 mm column, and diode array detection at 248 nm. Under the above conditions, the analysis time was below 8 min. Validation studies were based on U.S. Food and Drug Administration guidelines. The LOD (3 x SD criterion) was 15 ng/mL and LOQ (10 x SD criterion) was 70 ng/mL for disulfiram. The intraday and interday precisions were below 3.5%, recoveries were in the range of 97-102%, and robustness was below 3%. The optimized and validated micellar liquid chromatographic method was successfully applied to the determination of disulfiram in ayurvedic, herbal, divine ash, and other samples. The procedure developed could also be used in the fields of QC, routine analysis, and pharmacokinetic studies.
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ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/94.4.1082