Bovine parvovirus uses clathrin-mediated endocytosis for cell entry

Entry events of bovine parvovirus (BPV) were studied. Transmission electron micrographs of infected cells showed virus particles in cytoplasmic vesicles. Chemical inhibitors that block certain aspects of the cellular machinery were employed to assess viral dependency upon those cellular processes. C...

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Bibliographic Details
Published in:	Journal of general virology Vol. 91; no. Pt 12; pp. 3032 - 3041
Main Authors:	DUDLEENAMJIL, Enkhmart, LIN, Chin-Yo, DREDGE, Devin, MURRAY, Byron K, ROBISON, Richard A, JOHNSON, F. Brent
Format:	Journal Article
Language:	English
Published:	Reading Society for General Microbiology 01-12-2010
Subjects:	Animals Biological and medical sciences Bocavirus - physiology Bocavirus - ultrastructure Bovine parvovirus Cattle Cells, Cultured Clathrin-Coated Vesicles - ultrastructure Clathrin-Coated Vesicles - virology Endocytosis Epithelial Cells Fundamental and applied biological sciences. Psychology Microbiology Microscopy, Electron, Transmission Miscellaneous Virology Virus Internalization Virus Bovine parvovirus Clathrin Endocytosis Parvovirus Parvoviridae Veterinary Parvovirinae
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Summary:	Entry events of bovine parvovirus (BPV) were studied. Transmission electron micrographs of infected cells showed virus particles in cytoplasmic vesicles. Chemical inhibitors that block certain aspects of the cellular machinery were employed to assess viral dependency upon those cellular processes. Chlorpromazine, ammonium chloride, chloroquine and bafilamicin A1 were used to inhibit acidification of endosomes and clathrin-associated endocytosis. Nystatin was used as an inhibitor of the caveolae pathway. Cytochalasin D and ML-7 were used to inhibit actin and myosin functions, respectively. Nocodazole and colchicine were employed to inhibit microtubule activity. Virus entry was assessed by measuring viral transcription using real-time PCR, synthesis of capsid protein and assembly of infectious progeny virus in the presence of inhibitor blockage. The results indicated that BPV entry into embryonic bovine trachael cells utilizes endocytosis in clathrin-coated vesicles, is dependent upon acidification, and appears to be associated with actin and microtubule dependency. Evidence for viral entry through caveolae was not obtained. These findings provide a fuller understanding of the early cell-entry events of the replication cycle for members of the genus Bocavirus.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0022-1317 1465-2099 1465-2099
DOI:	10.1099/vir.0.024133-0