Analysis of the immune response to Neospora caninum in a model of intragastric infection in mice

To study experimental Neospora caninum infection initiated at the gastrointestinal tract, Toll-like Receptor 4- and functional IL-12Rβ2 chain-deficient C57BL/10 ScCr mice were challenged intragastrically with 5 x 10⁶ N. caninum tachyzoites. All parasite-inoculated mice eventually died with dissemina...

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Bibliographic Details
Published in:	Parasite immunology Vol. 29; no. 1; pp. 23 - 36
Main Authors:	TEIXEIRA, L, BOTELHO, A.S, BATISTA, A.R, MEIRELES, C.S, RIBEIRO, A, DOMINGUES, H.S, CORREIA DA COSTA, J.M, CASTRO, A.G, FAUSTINO, A.M.R, VILANOVA, M
Format:	Journal Article
Language:	English
Published:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-01-2007 Wiley Blackwell Publishing Ltd
Subjects:	Animal Animals Antibodies Antibodies, Protozoan - biosynthesis Ciências Médicas Coccidiosis Coccidiosis - immunology Coccidiosis - parasitology Coccidiosis - pathology Disease Models Disease Models, Animal Foxp3 IFN-γ IL-10 Inbred BALB C Inbred C57BL Intragastric infection Medicina Básica Mice Mice, Inbred BALB C Mice, Inbred C57BL Models, Animal Neospora - growth & development Neospora - immunology Neospora - pathogenicity Neospora caninum Protozoan Science & Technology
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Summary:	To study experimental Neospora caninum infection initiated at the gastrointestinal tract, Toll-like Receptor 4- and functional IL-12Rβ2 chain-deficient C57BL/10 ScCr mice were challenged intragastrically with 5 x 10⁶ N. caninum tachyzoites. All parasite-inoculated mice eventually died with disseminated infection. In contrast, immunocompetent BALB/c mice challenged with 1 x 10⁷ N. caninum tachyzoites by the intragastric (i.g.) or the intraperitoneal (i.p.) route remained alive for at least 6 months. Expansion of splenic B- and T-cells, the latter displaying both activated and regulatory phenotypes, and increased levels of IFN-γ and IL-10 mRNA were detected in both groups of infected BALB/c mice compared with non-infected controls, whereas in the Peyer's patches only IFN-γ mRNA levels were found to be increased. Parasite-specific IgG1, IgG2a and IgA antibody levels were elevated in the sera of all infected mice, whereas increased N. caninum-specific IgA levels were detected in intestinal lavage fluids of i.g. challenged mice only. These results show that N. caninum infection can be successfully established in mice by i.g. administration of tachyzoites. They also show that the immune response elicited in i.g. or i.p. infected BALB/c mice, although conferring some degree of protection, was not sufficient for complete parasite clearance.
Bibliography:	http://dx.doi.org/10.1111/j.1365-3024.2006.00911.x ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0141-9838 1365-3024
DOI:	10.1111/j.1365-3024.2006.00911.x