Retinoblastoma protein expression leads to reduced Oct-1 DNA binding activity and enhances interleukin-8 expression

Tumor cell lines with a defective retinoblastoma gene are unable to transcribe the HLA class II genes in response to IFN-gamma treatment, and reconstitution of functional Rb rescues IFN-gamma-induced class II gene expression. However, the molecular mechanism of Rb rescue of the class II genes is unk...

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Bibliographic Details
Published in:	Cell growth & differentiation Vol. 10; no. 7; pp. 457 - 465
Main Authors:	HONGQUAN ZHANG, SHEPHERD, A. T, EASON, D. D, SHENG WEI, DIAZ, J. I, DIEU, J. Y, WU, G. D, BLANCK, G
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA American Association for Cancer Research 01-07-1999
Subjects:	Biological and medical sciences Blotting, Western Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Chemotaxis, Leukocyte Culture Media DNA-Binding Proteins - metabolism Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Gene Expression Regulation HLA-DR alpha-Chains HLA-DR Antigens - metabolism Host Cell Factor C1 Humans In Vitro Techniques Interleukin-8 - biosynthesis Molecular and cellular biology Neutrophils - physiology Octamer Transcription Factor-1 Phosphorylation Retinoblastoma Protein - biosynthesis Retinoblastoma Protein - genetics Transcription Factors - metabolism Transfection Tumor Cells, Cultured Human Phosphorylation Class II histocompatibility antigen Retinoblastoma protein Gene expression Carcinogenesis Regulation(control) Cell line Urinary bladder Transcription factor Tumor cell Interleukin 8 Tumor suppressor gene
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Summary:	Tumor cell lines with a defective retinoblastoma gene are unable to transcribe the HLA class II genes in response to IFN-gamma treatment, and reconstitution of functional Rb rescues IFN-gamma-induced class II gene expression. However, the molecular mechanism of Rb rescue of the class II genes is unknown. We have examined the effect of Rb expression on the activation of the promoter for HLA-DRA, the prototype class II gene. Oct-1, a POU domain transcription factor, was identified as a repressor of HLA-DRA promoter activity in the Rb-defective cells. Rb expression led to phosphorylation of Oct-1, thus relieving its repressive effect. Oct-1 has also been shown to repress interleukin 8 promoter activity. Consistent with reduced levels of Oct-1 DNA binding activity in the Rb-transformed cell lines, interleukin 8 expression is higher in these cell lines.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1044-9523 2377-0732