Search Results - "DERTINGER, Stephen D"

Genotoxic mode of action predictions from a multiplexed flow cytometric assay and a machine learning approach by Bryce, Steven M., Bernacki, Derek T., Bemis, Jeffrey C., Dertinger, Stephen D.

“…Several endpoints associated with cellular responses to DNA damage as well as overt cytotoxicity were multiplexed into a miniaturized, “add and read” type flow…”
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The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup by Gollapudi, B. Bhaskar, Lynch, Anthony M., Heflich, Robert H., Dertinger, Stephen D., Dobrovolsky, Vasily N., Froetschl, Roland, Horibata, Katsuyoshi, Kenyon, Michelle O., Kimoto, Takafumi, Lovell, David P., Stankowski, Leon F., White, Paul A., Witt, Kristine L., Tanir, Jennifer Y.

“…•An IWGT Workgroup reviewed the in vivo Pig-a assay as a safety assessment tool.•Data for 41 agents evaluated in the rat Pig-a assay were…”
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Lack of hydroxyurea‐associated mutagenesis in pediatric sickle cell disease patients by Torous, Dorothea K., Avlasevich, Svetlana, Bemis, Jeffrey C., Howard, Thad, Ware, Russell E., Fung, Chunkit, Chen, Yuhchyau, Sahsrabudhe, Deepak, MacGregor, James T., Dertinger, Stephen D.

“…Hydroxyurea is approved for treating children and adults with sickle cell anemia (SCA). Despite its proven efficacy, concerns remain about its mutagenic and…”
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When pigs fly: Immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysis by Dertinger, Stephen D., Bryce, Steven M., Phonethepswath, Souk, Avlasevich, Svetlana L.

“…In vivo mutation assays based on the Pig-a null phenotype, that is, the absence of cell surface glycosylphosphatidylinositol (GPI) anchored proteins such as…”
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Quantitative in vitro to in vivo extrapolation of genotoxicity data provides protective estimates of in vivo dose by Beal, Marc A., Audebert, Marc, Barton‐Maclaren, Tara, Battaion, Hannah, Bemis, Jeffrey C., Cao, Xuefei, Chen, Connie, Dertinger, Stephen D., Froetschl, Roland, Guo, Xiaoqing, Johnson, George, Hendriks, Giel, Khoury, Laure, Long, Alexandra S., Pfuhler, Stefan, Settivari, Raja S., Wickramasuriya, Shamika, White, Paul

“…Genotoxicity assessment is a critical component in the development and evaluation of chemicals. Traditional genotoxicity assays (i.e., mutagenicity,…”
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Efficient monitoring of in vivo pig-a gene mutation and chromosomal damage: summary of 7 published studies and results from 11 new reference compounds by Dertinger, Stephen D, Phonethepswath, Souk, Avlasevich, Svetlana L, Torous, Dorothea K, Mereness, Jared, Bryce, Steven M, Bemis, Jeffrey C, Bell, Sara, Weller, Pamela, Macgregor, James T

“…The ability to effectively monitor gene mutation and micronucleated reticulocyte (MN-RET) frequency in short-term and repeated dosing schedules was…”
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Interlaboratory evaluation of a multiplexed high information content in vitro genotoxicity assay by Bryce, Steven M., Bernacki, Derek T., Bemis, Jeffrey C., Spellman, Richard A., Engel, Maria E., Schuler, Maik, Lorge, Elisabeth, Heikkinen, Pekka T., Hemmann, Ulrike, Thybaud, Véronique, Wilde, Sabrina, Queisser, Nina, Sutter, Andreas, Zeller, Andreas, Guérard, Melanie, Kirkland, David, Dertinger, Stephen D.

“…We previously described a multiplexed in vitro genotoxicity assay based on flow cytometric analysis of detergent‐liberated nuclei that are simultaneously…”
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The in vivo pig-a gene mutation assay, a potential tool for regulatory safety assessment by Dobrovolsky, Vasily N., Miura, Daishiro, Heflich, Robert H., Dertinger, Stephen D.

“…The Pig‐a (phosphatidylinositol glycan, Class A) gene codes for a catalytic subunit of the N‐acetylglucosamine transferase complex involved in an early step of…”
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In vivo mutation assay based on the endogenous Pig-a locus by Bryce, Steven M, Bemis, Jeffrey C, Dertinger, Stephen D

“…The product of the X-chromosome's Pig-a gene acts in the first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis, and is thereby essential for…”
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In vivo delivery of FTY720 prevents radiation-induced ovarian failure and infertility in adult female nonhuman primates by Zelinski, Mary B., Ph.D, Murphy, Mark K., M.S, Lawson, Maralee S., B.S, Jurisicova, Andrea, Ph.D, Pau, K. Y. Francis, Ph.D, Toscano, Natalia P., B.S, Jacob, Darla S., B.S, Fanton, John K., D.V.M, Casper, Robert F., M.D, Dertinger, Stephen D., Ph.D, Tilly, Jonathan L., Ph.D

“…Objective To determine whether sphingosine-1-phosphate (S1P), or the S1P mimetic FTY720 shields ovaries of adult female rhesus monkeys from damage caused by 15…”
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High information content assays for genetic toxicology testing: A report of the International Workshops on Genotoxicity Testing (IWGT) by Dertinger, Stephen D., Totsuka, Yukari, Bielas, Jason H., Doherty, Ann T., Kleinjans, Jos, Honma, Masamitsu, Marchetti, Francesco, Schuler, Maik J., Thybaud, Veronique, White, Paul, Yauk, Carole L.

“…•The IWGT evaluated high information content data streams for genotoxicity testing.•Strengths, weaknesses, opportunities, and threats were…”
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In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity by Bryce, Steven M., Bemis, Jeffrey C., Avlasevich, Svetlana L., Dertinger, Stephen D.

“…This laboratory has previously reported on the development of a flow cytometry-based method for scoring in vitro micronuclei in mouse lymphoma (L5178Y) cells…”
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Pig-a Mutation: Kinetics in Rat Erythrocytes Following Exposure to Five Prototypical Mutagens by Phonethepswath, Souk, Franklin, Dean, Torous, Dorothea K., Bryce, Steven M., Bemis, Jeffrey C., Raja, Sarojini, Avlasevich, Svetlana, Weller, Pamela, Hyrien, Ollivier, Palis, James, MacGregor, James T., Dertinger, Stephen D.

“…An in vivo mutation assay has been developed based on flow cytometric enumeration of glycosylphosphatidylinositol (GPI) anchor–deficient rat erythrocytes. With…”
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Persistence of cisplatin-induced mutagenicity in hematopoietic stem cells: implications for secondary cancer risk following chemotherapy by Dertinger, Stephen D, Avlasevich, Svetlana L, Torous, Dorothea K, Bemis, Jeffrey C, Phonethepswath, Souk, Labash, Carson, Carlson, Kristine, Mereness, Jared, Cottom, John, Palis, James, MacGregor, James T

“…Cisplatin is a cytostatic agent used in the treatment of many types of cancer, but its use is associated with increased incidences of secondary leukemia. We…”
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Assessment of the genotoxic potential of azidothymidine in the comet, micronucleus, and Pig-a assay by Guérard, Melanie, Koenig, Julie, Festag, Matthias, Dertinger, Stephen D, Singer, Thomas, Schmitt, Georg, Zeller, Andreas

“…The genotoxic potential of azidothymidine (Zidovudine, AZT), chosen as a model compound for nucleotide analogs, was comprehensively assessed in vivo for gene…”
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Comparison of male versus female responses in the Pig-a mutation assay by Labash, Carson, Avlasevich, Svetlana L, Carlson, Kristine, Torous, Dorothea K, Berg, Ariel, Bemis, Jeffrey C, MacGregor, James T, Dertinger, Stephen D

“…Validation of the Pig-a gene mutation assay has been based mainly on studies in male rodents. To determine if the mutagen-induced responses of the X-linked…”
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International Pig-a gene mutation assay trial: Evaluation of transferability across 14 laboratories by Dertinger, Stephen D., Phonethepswath, Souk, Weller, Pamela, Nicolette, John, Murray, Joel, Sonders, Paul, Vohr, Hans-Werner, Shi, Jing, Krsmanovic, Ljubica, Gleason, Carol, Custer, Laura, Henwood, Andrew, Sweder, Kevin, Stankowski Jr, Leon F., Roberts, Daniel J., Giddings, Amanda, Kenny, Julia, Lynch, Anthony M., Defrain, Céline, Nesslany, Fabrice, van der Leede, Bas-jan M., Van Doninck, Terry, Schuermans, Ann, Tanaka, Kentaro, Hiwata, Yoshie, Tajima, Osamu, Wilde, Eleanor, Elhajouji, Azeddine, Gunther, William C., Thiffeault, Catherine J., Shutsky, Thomas J., Fiedler, Ronald D., Kimoto, Takafumi, Bhalli, Javed A., Heflich, Robert H., MacGregor, James T.

“…A collaborative international trial was conducted to evaluate the reproducibility and transferability of an in vivo mutation assay based on the enumeration of…”
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Pig-a gene mutation and micronucleated reticulocyte induction in rats exposed to tumorigenic doses of the leukemogenic agents chlorambucil, thiotepa, melphalan, and 1,3-propane sultone by Dertinger, Stephen D., Phonethepswath, Souk, Avlasevich, Svetlana L., Torous, Dorothea K., Mereness, Jared, Cottom, John, Bemis, Jeffrey C., MacGregor, James T.

“…To evaluate whether blood‐based genotoxicity endpoints can provide temporal and dose‐response data within the low‐dose carcinogenic range that could contribute…”
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Absence of hydroxyurea‐induced mutational effects supports higher utilisation for the treatment of sickle cell anaemia by Ware, Russell E., Dertinger, Stephen D.

“…Summary Hydroxyurea (hydroxycarbamide) is approved for treating both children and adults with sickle cell anaemia (SCA). Fetal haemoglobin (HbF) induction is…”
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Induction of Pig-a mutant erythrocytes in male and female rats exposed to 1,3-propane sultone, ethyl carbamate, or thiotepa by Labash, Carson, Carlson, Kristine, Avlasevich, Svetlana L., Berg, Ariel, Bemis, Jeffrey C., MacGregor, James T., Dertinger, Stephen D.

“…•To date Pig-a gene mutation assays have overwhelmingly involved male rats.•Rats of both sexes were exposed to propane sultone, ethyl carbamate, or…”
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