Serodiagnosis of pulmonary tuberculosis in Argentina by enzyme-linked immunosorbent assay (ELISA) of IgG antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein derivative

IgG antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein derivative (PPD) was measured, by enzyme-linked immunosorbent assay (ELISA), in serum samples from 86 patients with active pulmonary tuberculosis and 91 non-tuberculous control subjects from Santa Fé, Argentina. The...

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Bibliographic Details
Published in:	Bulletin of the World Health Organization Vol. 62; no. 5; pp. 755 - 761
Main Authors:	BALESTRINO, E. A, DANIEL, T. M, DELATINI, M. D. S, LATINI, O. A, MA, Y, SCOCOZZA, J. B
Format:	Journal Article
Language:	English
Published:	Genève Organisation mondiale de la santé 1984
Subjects:	Antigens, Bacterial - immunology Argentina Bacterial diseases Biological and medical sciences Enzyme-Linked Immunosorbent Assay Human bacterial diseases Humans Immunoglobulin G - analysis Infectious diseases Medical sciences Mycobacterium tuberculosis - immunology Pneumology Respiratory system : syndromes and miscellaneous diseases Tropical medicine Tuberculin - immunology Tuberculosis and atypical mycobacterial infections Tuberculosis, Pulmonary - diagnosis Argentina South America Human Argentina Respiratory disease Lung Diagnostic Mycobacterial infection Enzyme immunoassay Immunological method Infection Tuberculin PPD Tuberculosis Bacteriosis ELISA assay
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Summary:	IgG antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein derivative (PPD) was measured, by enzyme-linked immunosorbent assay (ELISA), in serum samples from 86 patients with active pulmonary tuberculosis and 91 non-tuberculous control subjects from Santa Fé, Argentina. The geometric mean titre for the tuberculosis patients was 74.6 with antigen 5 and 99.5 with PPD. In 91 control subjects the geometric mean titres were 3.6 and 15.6 respectively. Titres were not related to tuberculin reactor status or prior BCG vaccination. At a serum dilution end-point of 1:40, ELISA with antigen 5 had a sensitivity of 81.4% and a specificity of 93.4% for tuberculosis. At 1:40, ELISA with PPD showed a sensitivity of 82.6% and a specificity of 54.9% for tuberculosis. Applied at a serum dilution of 1:40 to a hypothetical model population with a tuberculosis prevalence of 2%, ELISA using antigen 5 would correctly classify 93.2% of persons and ELISA with PPD, 55.5%. At a dilution of 1:80, accuracy is increased to 99.3% with antigen 5 and 83.3% with PPD, but sensitivity decreases to 64.0% with antigen 5 and 72.1% with PPD. Thus, antigen 5 is more accurate than PPD for the diagnosis of tuberculosis using ELISA.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0042-9686 1564-0604