Oncocytic modifications in rectal adenocarcinomas after radio and chemotherapy

The purpose of the study is to highlight oncocytic modifications in rectal adenocarcinomas and evaluate a possible correlation with preoperative radiochemotherapy (RCT). Twenty-eight cases of advanced rectal carcinoma, treated preoperatively by 5-fluorouracil (200-225 mg/m(2)) and 44-46 Gy in 22-23...

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Bibliographic Details
Published in:	Virchows Archiv : an international journal of pathology Vol. 448; no. 4; pp. 442 - 448
Main Authors:	AMBROSINI-SPALTRO, Andrea, SALVI, Fabrizio, BETTS, Christine M, FREZZA, Giovanni P, PIEMONTESE, Antonio, DEL PRETE, Pietro, BALDONI, Cristina, FOSCHINI, Maria P, VIALE, Giuseppe
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-04-2006 Springer Nature B.V
Subjects:	Adenocarcinoma - drug therapy Adenocarcinoma - pathology Adenocarcinoma - radiotherapy Aged Antimetabolites, Antineoplastic - therapeutic use Biological and medical sciences Biomarkers, Tumor - analysis Cell Transformation, Neoplastic Cytoplasm - ultrastructure Female Fluorouracil - therapeutic use Humans Investigative techniques, diagnostic techniques (general aspects) Liver cirrhosis Male Medical sciences Neoplasm, Residual - pathology Oxyphil Cells - pathology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Radiotherapy, Adjuvant Rectal Neoplasms - drug therapy Rectal Neoplasms - pathology Rectal Neoplasms - radiotherapy Tumors Adenocarcinoma Modification Anatomic pathology Chemotherapy Mitochondria Treatment resistance Treatment Drug-resistance Oncocyte Rectum Malignant tumor
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Summary:	The purpose of the study is to highlight oncocytic modifications in rectal adenocarcinomas and evaluate a possible correlation with preoperative radiochemotherapy (RCT). Twenty-eight cases of advanced rectal carcinoma, treated preoperatively by 5-fluorouracil (200-225 mg/m(2)) and 44-46 Gy in 22-23 fractions, were studied. All patients underwent biopsy before RCT. Surgery was performed within 6 weeks after RCT. In all cases oncocytic modifications were searched for on hematoxylin and eosin (H&E) and at immunohistochemistry using an antimitochondrial antibody. In addition, in two cases, both pre- and post-RCT tissues were examined at electron microscopy. All tumors were adenocarcinomas. In pre-RCT biopsies, oncocytic changes were difficult to find on H&E, while the antimitochondrial antibody strongly stained numerous neoplastic cells (mean 48.4%). In post-RCT surgical specimens, oncocytic changes were detected in 24 out of 28 cases on H&E and the antimitochondrial antibody stained most of the residual neoplastic cells (mean 76.7%). Ultrastructural examination revealed large and bizarre mitochondria inside tumor cells both in pre- and post-RCT tissues. In conclusion, the present data suggest that rectal adenocarcinomas are "mitochondrion-rich" tumors. After preoperative RCT, residual neoplastic cells acquire a definite oncocytic phenotype.
ISSN:	0945-6317 1432-2307
DOI:	10.1007/s00428-005-0137-6