Search Results - "DARZYNKIEWICZ, EDWARD"

Characterization of hMTr1, a Human Cap1 2′-O-Ribose Methyltransferase by Bélanger, François, Stepinski, Janusz, Darzynkiewicz, Edward, Pelletier, Jerry

“…Cellular eukaryotic mRNAs are capped at their 5′ ends with a 7-methylguanosine nucleotide, a structural feature that has been shown to be important for…”
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miR-483-5p orchestrates the initiation of protein synthesis by facilitating the decrease in phosphorylated Ser209eIF4E and 4E-BP1 levels by Nagaraj, Siranjeevi, Stankiewicz-Drogon, Anna, Darzynkiewicz, Edward, Wojda, Urszula, Grzela, Renata

“…Eukaryotic initiation factor 4E (eIF4E) is a pivotal protein involved in the regulatory mechanism for global protein synthesis in both physiological and…”
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Insight into the Binding and Hydrolytic Preferences of hNudt16 Based on Nucleotide Diphosphate Substrates by Chrabąszczewska, Magdalena, Winiewska-Szajewska, Maria, Ostrowska, Natalia, Bojarska, Elżbieta, Stępiński, Janusz, Mancewicz, Łukasz, Łukaszewicz, Maciej, Trylska, Joanna, Taube, Michał, Kozak, Maciej, Darżynkiewicz, Edward, Grzela, Renata

“…Nudt16 is a member of the NUDIX family of hydrolases that show specificity towards substrates consisting of a nucleoside diphosphate linked to another moiety…”
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MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the Cap-Binding Complex eIF4F by Mathonnet, Géraldine, Fabian, Marc R, Svitkin, Yuri V, Parsyan, Armen, Huck, Laurent, Murata, Takayuki, Biffo, Stefano, Merrick, William C, Darzynkiewicz, Edward, Pillai, Ramesh S, Filipowicz, Witold, Duchaine, Thomas F, Sonenberg, Nahum

“…MicroRNAs (miRNAs) play an important role in gene regulatory networks in animals. Yet, the mechanistic details of their function in translation inhibition or…”
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Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies by Dobieżyńska, Anna, Miszkiewicz, Joanna, Darżynkiewicz, Zbigniew M., Tyras, Michał, Stankiewicz-Drogoń, Anna, Trylska, Joanna, Darżynkiewicz, Edward, Grzela, Renata

“…The interferon-induced proteins with tetratricopeptide repeats (IFITs) are a family of RNA-binding proteins that are very highly expressed during antiviral…”
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Structural analysis of human 2′-O-ribose methyltransferases involved in mRNA cap structure formation by Smietanski, Miroslaw, Werner, Maria, Purta, Elzbieta, Kaminska, Katarzyna H., Stepinski, Janusz, Darzynkiewicz, Edward, Nowotny, Marcin, Bujnicki, Janusz M.

“…The 5′ cap of human messenger RNA contains 2′- O -methylation of the first and often second transcribed nucleotide that is important for its processing,…”
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CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures by Galloway, Alison, Atrih, Abdelmadjid, Grzela, Renata, Darzynkiewicz, Edward, Ferguson, Michael A J, Cowling, Victoria H

“…Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5' triphosphate. The cap guanosine and initial transcribed…”
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RNA sensor response in HeLa cells for transfected mRNAs prepared in vitro by SP6 and HiT7 RNA polymerases: A comparative study by Nagaraj, Siranjeevi, Stankiewicz-Drogon, Anna, Darzynkiewicz, Edward, Grzela, Renata

“…In vitro transcribed (IVT) synthetic mRNAs are in high demand due to their attractive bench to clinic translational processes. Mainly, the procedure to make…”
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Amino-Functionalized 5′ Cap Analogs as Tools for Site-Specific Sequence-Independent Labeling of mRNA by Warminski, Marcin, Sikorski, Pawel J, Warminska, Zofia, Lukaszewicz, Maciej, Kropiwnicka, Anna, Zuberek, Joanna, Darzynkiewicz, Edward, Kowalska, Joanna, Jemielity, Jacek

“…mRNA is a template for protein biosynthesis, and consequently mRNA transport, translation, and turnover are key elements in the overall regulation of gene…”
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Mesenchymal stem cell engineering by ARCA analog-capped mRNA by Andrzejewska, Anna, Grzela, Renata, Stankiewicz-Drogon, Anna, Rogujski, Piotr, Nagaraj, Siranjeevi, Darzynkiewicz, Edward, Lukomska, Barbara, Janowski, Miroslaw

“…We previously have shown that mRNA-based engineering may enhance mesenchymal stem cell (MSC) trafficking. However, optimal conditions for in vitro mRNA…”
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Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells by Grudzien-Nogalska, Ewa, Jemielity, Jacek, Kowalska, Joanna, Darzynkiewicz, Edward, Rhoads, Robert E

“…Capped RNAs synthesized by in vitro transcription have found wide utility for studying mRNA function and metabolism and for producing proteins of interest. We…”
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Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes by Kowalska, Joanna, Wypijewska del Nogal, Anna, Darzynkiewicz, Zbigniew M, Buck, Janina, Nicola, Corina, Kuhn, Andreas N, Lukaszewicz, Maciej, Zuberek, Joanna, Strenkowska, Malwina, Ziemniak, Marcin, Maciejczyk, Maciej, Bojarska, Elzbieta, Rhoads, Robert E, Darzynkiewicz, Edward, Sahin, Ugur, Jemielity, Jacek

“…Modified mRNA cap analogs aid in the study of mRNA-related processes and may enable creation of novel therapeutic interventions. We report the synthesis and…”
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Investigating the consequences of eIF4E2 (4EHP) interaction with 4E-transporter on its cellular distribution in HeLa cells by Kubacka, Dorota, Kamenska, Anastasiia, Broomhead, Helen, Minshall, Nicola, Darzynkiewicz, Edward, Standart, Nancy

“…In addition to the canonical eIF4E cap-binding protein, eukaryotes have evolved sequence-related variants with distinct features, some of which have been shown…”
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The potential of N2-modified cap analogues for precise genetic manipulation through mRNA engineering by Kurpiejewski, Karol, Stankiewicz-Drogon, Anna, Piecyk, Karolina, Rajkowska, Eliza, Skrzypczyk, Paulina, Geng, Jingping, Darzynkiewicz, Edward, Grzela, Renata, Jankowska-Anyszka, Marzena

“…The technology of mRNA-based drugs is currently being intensively developed and implemented. Medical products of this type are already being used as viral…”
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Five eIF4E isoforms from Arabidopsis thaliana are characterized by distinct features of cap analogs binding by Kropiwnicka, Anna, Kuchta, Krzysztof, Lukaszewicz, Maciej, Kowalska, Joanna, Jemielity, Jacek, Ginalski, Krzysztof, Darzynkiewicz, Edward, Zuberek, Joanna

“…•eIF4E, eIF4E1b and eIF4E1c interact with the cap stronger than eIF(iso)4E and nCBP.•Short loop between β7 and β8 strands causes low affinity of eIF(iso)4E for…”
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Drosophila miR2 primarily targets the m7GpppN cap structure for translational repression by Zdanowicz, Agnieszka, Thermann, Rolf, Kowalska, Joanna, Jemielity, Jacek, Duncan, Kent, Preiss, Thomas, Darzynkiewicz, Edward, Hentze, Matthias W

“…Understanding the molecular mechanism(s) of how miRNAs repress mRNA translation is a fundamental challenge in RNA biology. Here we use a validated cell-free…”
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Synthesis and properties of mRNA cap analogs containing imidodiphosphate moiety—fairly mimicking natural cap structure, yet resistant to enzymatic hydrolysis by Rydzik, Anna M., Kulis, Marta, Lukaszewicz, Maciej, Kowalska, Joanna, Zuberek, Joanna, Darzynkiewicz, Zbigniew M., Darzynkiewicz, Edward, Jemielity, Jacek

“…We describe synthesis and properties of eight dinucleotide mRNA 5′ cap analogs containing imidodiphosphate moiety within 5′,5′-tri- or tetraphosphate bridge…”
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Effect of the His-Tag Location on Decapping Scavenger Enzymes and Their Hydrolytic Activity toward Cap Analogs by Ferenc-Mrozek, Aleksandra, Bojarska, Elzbieta, Stepinski, Janusz, Darzynkiewicz, Edward, Lukaszewicz, Maciej

“…Decapping scavenger enzymes (DcpSs) are important players in mRNA degradation machinery and conserved in eukaryotes. Importantly, human DcpS is the recognized…”
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mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2 by Deshmukh, Mandar V., Jones, Brittnee N., Quang-Dang, Duc-Uy, Flinders, Jeremy, Floor, Stephen N., Kim, Candice, Jemielity, Jacek, Kalek, Marcin, Darzynkiewicz, Edward, Gross, John D.

“…Cap hydrolysis by Dcp2 is a critical step in several eukaryotic mRNA decay pathways. Processing requires access to cap-proximal nucleotides and the coordinated…”
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Biophysical Studies of eIF4E Cap-binding Protein: Recognition of mRNA 5′ Cap Structure and Synthetic Fragments of eIF4G and 4E-BP1 Proteins by Niedzwiecka, Anna, Marcotrigiano, Joseph, Stepinski, Janusz, Jankowska-Anyszka, Marzena, Wyslouch-Cieszynska, Aleksandra, Dadlez, Michal, Gingras, Anne-Claude, Mak, Pawel, Darzynkiewicz, Edward, Sonenberg, Nahum, Burley, Stephen K., Stolarski, Ryszard

“…mRNA 5′-cap recognition by the eukaryotic translation initiation factor eIF4E has been exhaustively characterized with the aid of a novel fluorometric,…”
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