Search Results - "DARDEL, Frederic"

Recombinant RNA technology: the tRNA scaffold by Dardel, Frédéric, Ponchon, Luc

“…RNA has emerged as a major player in most cellular processes. Understanding these processes at the molecular level requires homogeneous RNA samples for…”
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In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution by Ranaei-Siadat, Ehsan, Mérigoux, Cécile, Seijo, Bili, Ponchon, Luc, Saliou, Jean-Michel, Bernauer, Julie, Sanglier-Cianférani, Sarah, Dardel, Fréderic, Vachette, Patrice, Nonin-Lecomte, Sylvie

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Large scale expression and purification of recombinant RNA in Escherichia coli by Ponchon, Luc, Dardel, Frédéric

“…Stable, folded RNA are involved in many key cellular processes and can be used as tools for biological, pharmacological and/or molecular design studies…”
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A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold by Ponchon, Luc, Beauvais, Geneviève, Nonin-Lecomte, Sylvie, Dardel, Frédéric

“…RNA production using in vivo transcription by Escherichia coli allows preparation of milligram quantities of RNA for biochemical, biophysical and structural…”
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Co-expression of RNA-protein complexes in Escherichia coli and applications to RNA biology by Ponchon, Luc, Catala, Marjorie, Seijo, Bili, El Khouri, Marguerite, Dardel, Frédéric, Nonin-Lecomte, Sylvie, Tisné, Carine

“…RNA has emerged as a major player in many cellular processes. Understanding these processes at the molecular level requires homogeneous RNA samples for…”
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Investigation of RNA-Ligand Interactions by 19F NMR Spectroscopy Using Fluorinated Probes by Lombès, Thomas, Moumné, Roba, Larue, Valéry, Prost, Elise, Catala, Marjorie, Lecourt, Thomas, Dardel, Frédéric, Micouin, Laurent, Tisné, Carine

“…Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated…”
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Trapping conformational states along ligand-binding dynamics of peptide deformylase: the impact of induced fit on enzyme catalysis by Fieulaine, Sonia, Boularot, Adrien, Artaud, Isabelle, Desmadril, Michel, Dardel, Frédéric, Meinnel, Thierry, Giglione, Carmela

“…For several decades, molecular recognition has been considered one of the most fundamental processes in biochemistry. For enzymes, substrate binding is often…”
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Enzyme structural plasticity and the emergence of broad-spectrum antibiotic resistance by Maurice, Frédérique, Broutin, Isabelle, Podglajen, Isabelle, Benas, Philippe, Collatz, Ekkehard, Dardel, Frédéric

“…The emergence of multi‐resistant pathogenic bacteria is a worldwide health issue. Recently, clinical variants of a single antibiotic‐modifying…”
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In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution by Ranaei-Siadat, Ehsan, Mérigoux, Cécile, Seijo, Bili, Ponchon, Luc, Saliou, Jean-Michel, Bernauer, Julie, Sanglier-Cianférani, Sarah, Dardel, Fréderic, Vachette, Patrice, Nonin-Lecomte, Sylvie

“…TmRNA is an abundant RNA in bacteria with tRNA and mRNA features. It is specialized in trans-translation, a translation rescuing system. We demonstrate that…”
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unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis by Barraud, Pierre, Schmitt, Emmanuelle, Mechulam, Yves, Dardel, Frédéric, Tisné, Carine

“…In all organisms, translational initiation takes place on the small ribosomal subunit and two classes of methionine tRNA are present. The initiator is used…”
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RNA-methyltransferase TrmA is a dual-specific enzyme responsible for C 5 - methylation of uridine in both tmRNA and tRNA by Ranaei-Siadat, Ehsan, Fabret, Céline, Seijo, Bili, Dardel, Frédéric, Grosjean, Henri, Nonin-Lecomte, Sylvie

“…In bacteria, trans-translation rescues stalled ribosomes by the combined action of tmRNA (transfer-mRNA) and its associated protein SmpB. The tmRNA 5' and 3'…”
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Ribosome hijacking: a role for small protein B during trans-translation by Nonin-Lecomte, Sylvie, Germain-Amiot, Noella, Gillet, Reynald, Hallier, Marc, Ponchon, Luc, Dardel, Frédéric, Felden, Brice

“…Tight recognition of codon–anticodon pairings by the ribosome ensures the accuracy and fidelity of protein synthesis. In eubacteria, translational surveillance…”
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Discovery and Refinement of a New Structural Class of Potent Peptide Deformylase Inhibitors by Boularot, Adrien, Giglione, Carmela, Petit, Sylvain, Duroc, Yann, Alves de Sousa, Rodolphe, Larue, Valéry, Cresteil, Thierry, Dardel, Frédéric, Artaud, Isabelle, Meinnel, Thierry

“…New classes of antibiotics are urgently needed to counter increasing levels of pathogen resistance. Peptide deformylase (PDF) was originally selected as a…”
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NMR-guided fragment-based approach for the design of tRNA(Lys3) ligands by Chung, Florence, Tisné, Carine, Lecourt, Thomas, Dardel, Frédéric, Micouin, Laurent

“…Despite recent breakthroughs, the de novo design of new compounds that specifically bind to structured RNAs is still a standing challenge. The aim of this…”
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New insights into the formation of HIV-1 reverse transcription initiation complex by Barraud, Pierre, Gaudin, Cyril, Dardel, Frédéric, Tisné, Carine

“…HIV-1 reverse transcriptase uses the host tRNA 3 Lys as a primer for the synthesis of the minus DNA strand. The first event in viral replication is thus the…”
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Structure-Activity Relationship Analysis of the Peptide Deformylase Inhibitor 5-Bromo-1H-indole-3-acetohydroxamic Acid by Petit, Sylvain, Duroc, Yann, Larue, Valéry, Giglione, Carmela, Léon, Carole, Soulama, Coralie, Denis, Alexis, Dardel, Frédéric, Meinnel, Thierry, Artaud, Isabelle

“…SAR by NMR: A series of indole compounds derived from 5‐bromo‐1H‐indole‐3‐acetohydroxamic acid were synthesized. Their inhibitory activities were evaluated…”
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New peptide deformylase inhibitors and cooperative interaction: a combination to improve antibacterial activity by GOEMAERE, Emilie, MELET, Armelle, MEINNEL, Thierry, DARDEL, Frederic, ARTAUD, Isabelle, PAGES, Jean-Marie, LARUE, Valéry, LIEUTAUD, Aurélie, DE SOUSA, Rodolphe Alves, CHEVALIER, Jacqueline, YIMGA-DJAPA, Liselotte, GIGLIONE, Carmela, HUGUET, Florian, ALIMI, Mickael

“…Bacterial drug resistance is a worrying public health problem and there is an urgent need for research and development to provide new antibacterial molecules…”
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NMR-Guided Fragment-Based Approach for the Design of AAC(6')-Ib Ligands by Lombès, Thomas, Bégis, Guillaume, Maurice, Frédérique, Turcaud, Serge, Lecourt, Thomas, Dardel, Frédéric, Micouin, Laurent

“…Reverse n.O.e. pumping in the detection and optimisation of ligands: Ligand‐observed NMR experiments have been used to drive the detection and optimisation of…”
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NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation by Nonin-Lecomte, Sylvie, Felden, Brice, Dardel, Frédéric

“…The transfer-messenger RNA (tmRNA) pseudoknot PK1 is essential for bacterial trans-translation, a ribosomal rescue mechanism. We report the solution structure…”
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Design of tRNALys3 Ligands: Fragment Evolution and Linker Selection Guided by NMR Spectroscopy by Chung, Florence, Tisné, Carine, Lecourt, Thomas, Seijo, Bili, Dardel, Frédéric, Micouin, Laurent

“…A fragment‐based approach for the synthesis of ligands of tRNALys3, the HIV reverse‐transcription primer, is described. The use of NMR spectroscopy has proved…”
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