Search Results - "DANSON, Michael J."

Temperature and the catalytic activity of enzymes: A fresh understanding by Daniel, Roy M., Danson, Michael J.

“…The discovery of an additional step in the progression of an enzyme from the active to inactive state under the influence of temperature has led to a better…”
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The dependence of enzyme activity on temperature: determination and validation of parameters by Peterson, Michelle E, Daniel, Roy M, Danson, Michael J, Eisenthal, Robert

“…Traditionally, the dependence of enzyme activity on temperature has been described by a model consisting of two processes: the catalytic reaction defined by…”
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Catalytic efficiency and kcat / KM : a useful comparator? by Eisenthal, Robert, Danson, Michael J, Hough, David W

“…The ratio kcat / KM – often referred to as the ‘specificity constant’ – is a useful index for comparing the relative rates of an enzyme acting on alternative,…”
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The molecular basis of the effect of temperature on enzyme activity by Daniel, Roy M, Peterson, Michelle E, Danson, Michael J, Price, Nicholas C, Kelly, Sharon M, Monk, Colin R, Weinberg, Cristina S, Oudshoorn, Matthew L, Lee, Charles K

“…Experimental data show that the effect of temperature on enzymes cannot be adequately explained in terms of a two-state model based on increases in activity…”
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Inhibition of extracellular proteases improves the production of a xylanase in Parageobacillus thermoglucosidasius by Holland, Alexandria T N, Danson, Michael J, Bolhuis, Albert

“…Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose and pentose sugars for fermentation. The…”
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Metabolism of Pentose Sugars in the Hyperthermophilic Archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius by Nunn, Charlotte E.M., Johnsen, Ulrike, Schönheit, Peter, Fuhrer, Tobias, Sauer, Uwe, Hough, David W., Danson, Michael J.

“…We have previously shown that the hyperthermophilic archaeon, Sulfolobus solfataricus, catabolizes d-glucose and d-galactose to pyruvate and glyceraldehyde via…”
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Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp by Bacon, Leann F, Hamley-Bennett, Charlotte, Danson, Michael J, Leak, David J

“…Geobacillus thermoglucosidasius is a thermophilic, natural ethanol producer and a potential candidate for commercial bioethanol production. Previously, G…”
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Why are the 2-oxoacid dehydrogenase complexes so large? Generation of an active trimeric complex by Marrott, Nia L, Marshall, Jacqueline J T, Svergun, Dmitri I, Crennell, Susan J, Hough, David W, van den Elsen, Jean M H, Danson, Michael J

“…The four-component polypeptides of the 2-oxoacid dehydrogenase complex from the thermophilic archaeon Thermoplasma acidophilum assemble to give an active…”
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Syntheses of 2-Keto-3-deoxy-D-xylonate and 2-Keto-3-deoxy-L-arabinonate as Stereochemical Probes for Demonstrating the Metabolic Promiscuity of Sulfolobus solfataricus Towards D-Xylose and L-Arabinose by Archer, Robert M., Royer, Sylvain F., Mahy, William, Winn, Caroline L., Danson, Michael J., Bull, Steven D.

“…Practical syntheses of 2‐keto‐3‐deoxy‐D‐xylonate (D‐KDX) and 2‐keto‐3‐deoxy‐L‐arabinonate (L‐KDA) that rely on reaction of the anion of ethyl…”
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Structurally Informed Site-Directed Mutagenesis of a Stereochemically Promiscuous Aldolase To Afford Stereochemically Complementary Biocatalysts by Royer, Sylvain F., Haslett, Luke, Crennell, Susan J., Hough, David W., Danson, Michael J., Bull, Steven D.

“…2-Keto-3-deoxygluconate aldolase from the hyperthermophile Sulfolobus solfataricus is a highly thermostable type I aldolase that can catalyze carbon−carbon…”
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Engineering enhanced thermostability into the Geobacillus pallidus nitrile hydratase by Van Wyk, Jennifer C., Sewell, B. Trevor, Danson, Michael J., Tsekoa, Tsepo L., Sayed, Muhammed F., Cowan, Don A.

“…Nitrile hydratases (NHases) are important biocatalysts for the enzymatic conversion of nitriles to industrially-important amides such as acrylamide and…”
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Comparative genomic analysis reveals 2-oxoacid dehydrogenase complex lipoylation correlation with aerobiosis in archaea by Borziak, Kirill, Posner, Mareike G, Upadhyay, Abhishek, Danson, Michael J, Bagby, Stefan, Dorus, Steve

“…Metagenomic analyses have advanced our understanding of ecological microbial diversity, but to what extent can metagenomic data be used to predict the…”
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Post-translational modification in the archaea: structural characterization of multi-enzyme complex lipoylation by Posner, Mareike G, Upadhyay, Abhishek, Crennell, Susan J, Watson, Andrew J A, Dorus, Steve, Danson, Michael J, Bagby, Stefan

“…Lipoylation, the covalent attachment of lipoic acid to 2-oxoacid dehydrogenase multi-enzyme complexes, is essential for metabolism in aerobic bacteria and…”
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Metabolic Pathway Promiscuity in the Archaeon Sulfolobus solfataricus Revealed by Studies on Glucose Dehydrogenase and 2-Keto-3-deoxygluconate Aldolase by Lamble, Henry J., Heyer, Narinder I., Bull, Steven D., Hough, David W., Danson, Michael J.

“…The hyperthermophilic Archaeon Sulfolobus solfataricus metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway…”
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A New Intrinsic Thermal Parameter for Enzymes Reveals True Temperature Optima by Peterson, Michelle E, Eisenthal, Robert, Danson, Michael J, Spence, Alastair, Daniel, Roy M

“…Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of…”
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The Structural Basis of Substrate Promiscuity in Glucose Dehydrogenase from the Hyperthermophilic Archaeon Sulfolobus solfataricus by Milburn, Christine C., Lamble, Henry J., Theodossis, Alex, Bull, Steven D., Hough, David W., Danson, Michael J., Taylor, Garry L.

“…The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 °C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff…”
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The Structural Basis for Substrate Promiscuity in 2-Keto-3-deoxygluconate Aldolase from the Entner-Doudoroff Pathway in Sulfolobus solfataricus by Theodossis, Alex, Walden, Helen, Westwick, Elaine J, Connaris, Helen, Lamble, Henry J, Hough, David W, Danson, Michael J, Taylor, Garry L

“…The hyperthermophilic Archaea Sulfolobus solfataricus grows optimally above 80 °C and metabolizes glucose by a non-phosphorylative variant of the…”
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Gluconate dehydratase from the promiscuous Entner–Doudoroff pathway in Sulfolobus solfataricus by Lamble, Henry J., Milburn, Christine C., Taylor, Garry L., Hough, David W., Danson, Michael J.

“…An investigation has been carried out into gluconate dehydratase from the hyperthermophilic Archaeon Sulfolobus solfataricus. The enzyme has been purified from…”
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Promiscuity in the part-phosphorylative Entner–Doudoroff pathway of the archaeon Sulfolobus solfataricus by Lamble, Henry J., Theodossis, Alex, Milburn, Christine C., Taylor, Garry L., Bull, Steven D., Hough, David W., Danson, Michael J.

“…The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a ‘promiscuous’ non-phosphorylative variant of the Entner–Doudoroff…”
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Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium by Russell, Rupert JM, Gerike, Ursula, Danson, Michael J, Hough, David W, Taylor, Garry L

“…Background: The structural basis of adaptation of enzymes to low temperature is poorly understood. Dimeric citrate synthase has been used as a model enzyme to…”
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