Evaluation of Schistosoma japonicum thioredoxin peroxidase-1 as a potential circulating antigen target for the diagnosis of Asian schistosomiasis

Evaluation of Schistosoma japonicum thioredoxin peroxidase-1 as a potential circulating antigen target for the diagnosis of Asian schistosomiasis

Asian schistosomiasis caused by Schistosoma japonicum is a serious zoonotic disease endemic in China, the Philippines and parts of Indonesia. Mass drug administration in endemic areas resulted to decline in disease severity and intensity. The low intensity of infection limits the use of current para...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science Vol. 80; no. 1; pp. 156 - 163
Main Authors: MACALANDA, Adrian Miki C., ANGELES, Jose Ma. M., MOENDEG, Kharleezelle J., DANG, Anh TM, HIGUCHI, Luna, INOUE, Noboru, XUAN, Xuenan, KIRINOKI, Masashi, CHIGUSA, Yuichi, LEONARDO, Lydia R., VILLACORTE, Elena A., RIVERA, Pilarita T., GOTO, Yasuyuki, KAWAZU, Shin-ichiro
Format: Journal Article
Language:English
Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-01-2018
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects:
Antigens
Antioxidants
Biomarkers
Diagnosis
Enzyme-linked immunosorbent assay
Infections
Monoclonal antibodies
Parasites
Parasitology
Peroxidase
Peroxiredoxin
Sandwich ELISA
Schistosoma japonicum
Schistosomiasis
schistosomiasis biomarker
Thioredoxin
Thioredoxin peroxidase
thioredoxin peroxidase-1
Zoonoses
thioredoxin peroxidase-1
Schistosoma japonicum
Sandwich ELISA
schistosomiasis biomarker
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Summary:Asian schistosomiasis caused by Schistosoma japonicum is a serious zoonotic disease endemic in China, the Philippines and parts of Indonesia. Mass drug administration in endemic areas resulted to decline in disease severity and intensity. The low intensity of infection limits the use of current parasitological methods for schistosomiasis diagnosis. Detection of parasite circulating antigens might provide more informative result as it may indicate the true status of infection. In this study, S. japonicum thioredoxin peroxidase-1 (SjTPx-1) a 22 kDa secreted antioxidant enzyme expressed throughout the life stages of the parasite was evaluated for its potential use as a biomarker for schistosomiasis japonica infection. Rabbit polyclonal antibody and mouse monoclonal antibodies (mAbs) were raised against the recombinant SjTPx-1 (rSjTPx-1). The antibodies produced against the recombinant antigen was confirmed to detect the native SjTPx-1 in crude adult worm lysate. Likewise, the specific binding of mAbs to parasite TPx-1 and not to mammalian peroxiredoxin-1 orthologues was also confirmed. The double antibody sandwich ELISA developed in this study was able to detect at least 1 ng/ml of rSjTPx-1. In addition, this method was able to detect the antigen from all serum samples of experimentally infected rabbit and mice. The diagnostic potential of SjTPx-1 in human clinical samples was also evaluated, in which 4 out of 10 stool-confirmed serum samples had detectable levels of the antigen. The results suggest that SjTPx-1 can be a potential biomarker for Asian zoonotic schistosomiasis.
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.17-0579