Development of a chemiluminescent optical fiber immunosensor to detect Streptococcus pneumoniae antipolysaccharide antibodies

A chemiluminescent-based optical fiber immunosensor was developed for the detection of antipneumococcal antibodies. This was accomplished by developing a different chemical procedure utilizing 3-aminopropyl trimethoxysilane and cyanuric chloride to conjugate pneumococcal cell wall polysaccharides to...

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Bibliographic Details
Published in:	Applied biochemistry and biotechnology Vol. 89; no. 2-3; pp. 117 - 126
Main Authors:	MARKS, Robert S, MARGALIT, Alon, BYCHENKO, Alexei, BASSIS, Efim, PORAT, Nurith, DAGAN, R. O. N
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-11-2000 Springer Nature B.V
Subjects:	3-aminopropyl trimethoxysilane Antibodies - chemistry Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Chemiluminescence cyanuric chloride Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Fiber Optic Technology Fundamental and applied biological sciences. Psychology Immune system Immunoassay Luminescent Measurements Methods. Procedures. Technologies Models, Chemical Optical Fibers Pneumonia polysaccharides Polysaccharides - chemistry Polysaccharides - immunology Propylamines - chemistry Saccharides Saliva - chemistry Sensitivity and Specificity Sensors Silanes - chemistry Streptococcus infections Streptococcus pneumoniae Streptococcus pneumoniae - immunology Studies Triazines - chemistry Urine - chemistry Various methods and equipments Streptococcaceae Antibody Biosensor Pathogenic Bacteria Micrococcales Capsule Chemiluminescence Polysaccharide Immunosensor Optical fiber Streptococcus pneumoniae
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Summary:	A chemiluminescent-based optical fiber immunosensor was developed for the detection of antipneumococcal antibodies. This was accomplished by developing a different chemical procedure utilizing 3-aminopropyl trimethoxysilane and cyanuric chloride to conjugate pneumococcal cell wall polysaccharides to the optical fiber tips, and by improving the sensitivity of the photodetection system. The lowest titer of antipneumococcal antibodies detected by the optical fiber was at a 1:819,200 dilution. The lowest corresponding value by standard enzyme-linked immunosorbent assay was at a 1:98,415 dilution. It was concluded that the optical immunosensor system is an accurate and sensitive method to detect antipneumococcal antibodies and may be an adequate tool to monitor antibodies in specimens such as saliva and urine.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-2 ObjectType-Feature-1 content type line 23 SourceType-Conference Papers & Proceedings-1 ObjectType-Conference-3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2
ISSN:	0273-2289 1559-0291 0273-2289
DOI:	10.1385/ABAB:89:2-3:117