The Shape Parameter of Liposomes and DNA-Lipid Complexes Determined by Viscometry Utilizing Small Sample Volumes

The Shape Parameter of Liposomes and DNA-Lipid Complexes Determined by Viscometry Utilizing Small Sample Volumes

A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/ b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/−) was 3.7...

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Bibliographic Details
Published in:Biophysical journal Vol. 85; no. 2; pp. 1223 - 1232
Main Authors: Sun, Y., Li, X., Düzgüneşν, N., Takaoka, Y., Ohi, S., Hirota, S.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-08-2003
Biophysical Society
Subjects:
Capillary Action
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - ultrastructure
Drug Delivery Systems - methods
Equipment Design
Equipment Failure Analysis - instrumentation
Equipment Failure Analysis - methods
Gene therapy
Lipids - chemistry
Liposomes - chemistry
Macromolecular Substances
Materials Testing - instrumentation
Materials Testing - methods
Membrane Fluidity
Microchemistry - instrumentation
Microchemistry - methods
Microfluidics - instrumentation
Microfluidics - methods
Molecular Conformation
Particle Size
Reproducibility of Results
Sample Size
Sensitivity and Specificity
Supramolecular Assemblies
Viscosity
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Summary:A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/ b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/−) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.
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content type line 23
Address reprint requests to S. Hirota, 6-6-18 Higashikaigan-Minami, Chigasaki, 253-0054 Japan. Tel.: 81-467-85-8471; Fax: 81-467-57-5080; E-mail: sadaohiro@aol.com.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(03)74558-5