Genomic analysis and proteomic response of the chromium‐resistant and phenanthrene‐degrading strain Streptomyces sp. MC1

Genomic analysis and proteomic response of the chromium‐resistant and phenanthrene‐degrading strain Streptomyces sp. MC1

Aim Chemically disparate toxic organic and/or inorganic molecules produced by anthropogenic activities often hinder the bioremediation process. This research was conducted to understand the capacity of Streptomyces sp. MC1 to remove chemically disparate toxics such as Cr(VI) or phenanthrene. Methods...

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Bibliographic Details
Published in:Journal of applied microbiology Vol. 131; no. 2; pp. 719 - 727
Main Authors: Sineli, P.E., Herrera, H.M., Aparicio, J.D., Guerrero, D.S., Polti, M.A., Dávila Costa, J.S.
Format: Journal Article
Language:English
Published: England Oxford University Press 01-08-2021
Subjects:
Anthropogenic factors
Biodegradation
Bioremediation
Chromium
Contaminants
Degradation
Genomic analysis
genomics
Human influences
Intermediates
Metabolism
Metabolites
Phenanthrene
Proteomics
Streptomyces
Streptomyces
biodegradation
genomics
bioremediation
proteomics
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Summary:Aim Chemically disparate toxic organic and/or inorganic molecules produced by anthropogenic activities often hinder the bioremediation process. This research was conducted to understand the capacity of Streptomyces sp. MC1 to remove chemically disparate toxics such as Cr(VI) or phenanthrene. Methods and Results Genomic, metabolic modeling and proteomic approaches were used in this study. Our results demonstrated that Streptomyces sp. MC1 has the genetic determinants to remove Cr(VI) or degrade phenanthrene. Proteomics showed that these genetic determinants were expressed. Metabolic versatility of the strain was confirmed by two metabolic models in complex and minimal media. Interestingly, our results also suggested a connection between the degradation of phenanthrene and synthesis of specialized metabolites. Conclusions Streptomyces sp. MC1 has the genetic and physiological potential to remove Cr(VI) or degrade phenanthrene Significance and Impact of Study The probability of a microorganism to survive in the presence of different contaminants depends on its genetic potential and the ability to express it. The genetic and proteomic profiles obtained for Streptomyces sp. MC1 can be recommended as model and predict if other Streptomyces strains can be used in bioremediation processes. Our work also hypothesized that intermediates of the phenanthrene degradation serve as precursors for the specialized metabolism.
Bibliography:Pedro E. Sineli and Hector M. Herrera contributed equally to this work.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.15002