A GRA1 DNA Vaccine Primes Cytolytic CD8 super(+) T Cells To Control Acute Toxoplasma gondii Infection

A GRA1 DNA Vaccine Primes Cytolytic CD8 super(+) T Cells To Control Acute Toxoplasma gondii Infection

Protective immunity against Toxoplasma gondii is known to be mediated mainly by T lymphocytes and gamma interferon (IFN-[gamma]). The contribution of CD4 super(+) and CD8 super(+) T-lymphocyte subsets to protective immune responses against T. gondii infection, triggered by a GRA1 (p24) DNA vaccine,...

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Bibliographic Details
Published in:Infection and immunity Vol. 71; no. 1; pp. 309 - 316
Main Authors: D'Souza, TSAS, Laloup, M, Dewit, J, De Braekeleer, J, Verschueren, H, Huygen, MVK, Jongert, E
Format: Journal Article
Language:English
Published: 01-01-2003
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Summary:Protective immunity against Toxoplasma gondii is known to be mediated mainly by T lymphocytes and gamma interferon (IFN-[gamma]). The contribution of CD4 super(+) and CD8 super(+) T-lymphocyte subsets to protective immune responses against T. gondii infection, triggered by a GRA1 (p24) DNA vaccine, was assessed in this study. In vitro T-cell depletion experiments indicated that both CD4 super(+) and CD8 super(+) T-cell subsets produced IFN-[gamma] upon restimulation with a T. gondii lysate. In addition, the GRA1 DNA vaccine elicited CD8 super(+) T cells that were shown to have cytolytic activity against parasite-infected target cells and a GRA1-transfected cell line. C3H mice immunized with the GRA1 DNA vaccine showed 75 to 100% protection, while 0 to 25% of the mice immunized with the empty control vector survived challenge with T. gondii cysts. In vivo T-cell depletion experiments indicated that CD8 super(+) T cells were essential for the survival of GRA1-vaccinated C3H mice during the acute phase of T. gondii infection, while depletion of CD4 super(+) T cells led to an increase in brain cyst burden during the chronic phase of infection.
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ISSN:0019-9567
DOI:10.1128/IAI.71.1.309-316.2003