Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks,...

Full description

Saved in:
Bibliographic Details
Published in:Parasites & vectors Vol. 14; no. 1; pp. 1 - 368
Main Authors: Sato, Kozue, Liu, Dan, Cui, Yunhong, Yin, Xuhong, Zhang, Lihua, Li, Hong, Wang, Tingfu, Liu, Rongxin, Wu, Lijing, Lu, Saixia, Gao, Ting, Zhang, Zitong, Cao, Minzhi, Wang, Guodong, Li, Chunpu, Yan, Dacheng, Ohashi, Norio, Ando, Shuji, Kawabata, Hiroki
Format: Journal Article
Language:English
Published: London BioMed Central Ltd 17-07-2021
BioMed Central
BMC
Subjects:
Arachnids
Borrelia miyamotoi
Deoxyribonucleic acid
Disease transmission
DNA
Epidemiology
Fever
Gene sequencing
Genomes
Genomic analysis
Identification
Infections
Inner Mongolia
Ixodes persulcatus
Laboratories
Lyme disease
Maximum likelihood method
MLSA
Nucleotide sequence
Pathogens
PCR
Phosphodiesterase
Polymerase chain reaction
Relapsing fever
Risk factors
Sequence analysis
Sequencing
Software
Spirochetes
Statistics
Surveillance
Ticks
Vector-borne diseases
China
Inner Mongolia China
United States > US
Japan
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China. From May to June each year during the period 2016-2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA). A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia. We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-021-04809-z