Protein complexes associated with β‐catenin differentially influence the differentiation profile of neonatal and adult CD8+ T cells

The canonical Wnt signaling pathway is a master cell regulator involved in CD8+ T cell proliferation and differentiation. In human CD8+ T cells, this pathway induces differentiation into memory cells or a “stem cell memory like” population, which is preferentially present in cord blood. To better un...

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Published in:	Journal of cellular physiology Vol. 234; no. 10; pp. 18639 - 18652
Main Authors:	Hernández‐Acevedo, Gerson N., López‐Portales, Oscar H., Gutiérrez‐Reyna, Darely Y., Cuevas‐Fernández, Erick, Kempis‐Calanis, Linda A., Labastida‐Conde, Rosario G., Aguilar‐Luviano, Oscar B., Ramírez‐Pliego, Oscar, Spicuglia, Salvatore, Lino‐Alfaro, Bárbara, Chagolla‐López, Alicia, González‐de la Vara, Luis E., Santana, María Angélica
Format:	Journal Article
Language:	English
Published:	United States Wiley Subscription Services, Inc 01-10-2019 Wiley
Subjects:	Adult Adults beta Catenin - metabolism Blood Calcium Calcium ions Catenin CBP/p300 CD8 antigen CD8+ T cells CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - metabolism Cell activation Cell Differentiation Cell proliferation Cell self-renewal Chromatin Computer memory Cord blood Differentiation Event-related potentials Gene Expression Regulation Genes Humans Immunology Immunoprecipitation Infant, Newborn Life Sciences Lymphocytes Lymphocytes T Memory cells Metabolism Multiprotein Complexes - metabolism neonate immunity Neonates Polarity Polymerase chain reaction Promoter Regions, Genetic - genetics Protein Binding Protein Interaction Mapping Proteins proteomics Signal transduction Signaling Stem cells Wnt protein Wnt Signaling Pathway β‐catenin β-catenin CD8+ T cells neonate immunity proteomics CBP/p300 beta-catenin
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Summary:	The canonical Wnt signaling pathway is a master cell regulator involved in CD8+ T cell proliferation and differentiation. In human CD8+ T cells, this pathway induces differentiation into memory cells or a “stem cell memory like” population, which is preferentially present in cord blood. To better understand the role of canonical Wnt signals in neonatal or adult blood, we compared the proteins associated with β‐catenin, in nonstimulated and Wnt3a‐stimulated human neonatal and adult naive CD8+ T cells. Differentially recruited proteins established different complexes in adult and neonatal cells. In the former, β‐catenin‐associated proteins were linked to cell signaling and immunological functions, whereas those of neonates were linked to proliferation and metabolism. Wnt3a stimulation led to the recruitment and overexpression of Wnt11 in adult cells and Wnt5a in neonatal cells, suggesting a differential connexion with planar polarity and Wnt/Ca2+ noncanonical pathways, respectively. The chromatin immunoprecipitation polymerase chain reaction β‐catenin was recruited to a higher level on the promoters of cell renewal genes in neonatal cells and of differentiation genes in those of adults. We found a preferential association of β‐catenin with CBP in neonatal cells and with p300 in the adult samples, which could be involved in a higher self‐renewal capacity of the neonatal cells and memory commitment in those of adults. Altogether, our results show that different proteins associated with β‐catenin during Wnt3a activation mediate a differential response of neonatal and adult human CD8+ T cells. Specific proteins were found associated with β‐catenin in nonstimulated and Wnt3a‐stimulated human neonatal cells as compared with those of adults. Differentially recruited proteins established different complexes in adult and neonatal cells. These differential proteins could be responsible for a differential response of neonatal and adult human CD8+T cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9541 1097-4652
DOI:	10.1002/jcp.28502