Characterization of specific IgE response in vitro against protein and drug allergens using atopic and normal donors

Background: As the incidence of allergy to different compounds increases in society, the need to understand and characterize specific IgE responses becomes obvious. Different cell culture systems have been evaluated for their ability to support such IgE secretion. Methods: One system employed human...

Full description

Saved in:

Bibliographic Details
Published in:	Allergy (Copenhagen) Vol. 57; no. 3; pp. 193 - 200
Main Authors:	Åkesson, A., Ingvarsson, S., Karlsson, F., Leyva, L., Blanca, M., Cuerden, S. A., Smith, J. A., Coleman, J. W., Borrebaeck, C. A. K.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science, Ltd 01-03-2002 Blackwell
Subjects:	allergenicity Allergens - immunology Amino Acid Sequence B-Lymphocytes - immunology benzylpenicillin Biological and medical sciences Blood Donors Cells, Cultured Clinical Medicine grasspollen Humans Hypersensitivity - immunology IgE Immunoglobulin E - biosynthesis Immunoglobulin G - biosynthesis Immunological methods for diagnosis and exploration Immunopathology in vitro Interleukin-4 - pharmacology Interleukin-5 - pharmacology Klinisk medicin Lungmedicin och allergi Medical and Health Sciences Medical sciences Medicin och hälsovetenskap Molecular Sequence Data Penicillin G - immunology Pollen - immunology Respiratory Medicine and Allergy Serological tests Human Drug Immunopathology Allergy Monocotyledones Immune response Specific IgE β-Lactams Benzylpenicillin Allergenicity In vitro Atopy Penicillin derivatives Gramineae Angiospermae Spermatophyta Pollen Diagnosis Molecular biology ELISA assay Allergen
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Background: As the incidence of allergy to different compounds increases in society, the need to understand and characterize specific IgE responses becomes obvious. Different cell culture systems have been evaluated for their ability to support such IgE secretion. Methods: One system employed human peripheral lymphocytes (PBL) from normal donors stimulated with anti‐CD3 activated T cells with or without the presence of allergens like benzylpenicillin (BP) and Phlenum pratense (PP). Secretion of IgE was analyzed in ELISA and compared to the IgG response to the nonallergenic antigen tetanus toxoid (TT). Another system employed stimulation of T and B cells with a heterotope, consisting of a T helper cell epitope derived from TT, and a B cell allergen epitope derived from BP. The specific IgE secretion was compared, using lymphocytes from normal as well as BP‐allergic donors. Results: Anti‐CD3 stimulated T cells supported BP‐specific IgE secretion in six of 11 normal donors. This response was inhibited in four donors and enhanced in two donors by the addition of the BP‐allergen to the culture. In contrast, addition of the protein allergen (PP) or antigen (TT) to the same culture system inhibited both IgE and IgG synthesis in all experiments. Cells from the majority (10/16) of the BP‐allergic donors failed to produce BP‐specific IgE in vitro, when cultured in the presence of allergen. Conclusions: An allergen specific immune response is readily generated in vitro. The differential response against benzylpenicillin between different donor categories most probably reflects the level of pre‐exposure to this allergen in vivo.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0105-4538 1398-9995 1398-9995
DOI:	10.1034/j.1398-9995.2002.1o3321.x