Search Results - "Crout, David H.G"
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Immobilization/stabilization on Eupergit C of the β-galactosidase from B. circulans and an α-galactosidase from Aspergillus oryzae
Published in Enzyme and microbial technology (01-07-2000)“…Two synthetically useful glycosidases, the β-galactosidase from Bacillus circulans and an α-galactosidase from Aspergillus oryzae have been immobilized on…”
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Glycosidase-catalysed synthesis of mannobioses by the reverse hydrolysis activity of α-mannosidase: partial purification of α-mannosidases from almond meal, limpets and Aspergillus niger
Published in Tetrahedron: asymmetry (28-01-2000)“…Two disaccharides, α- d-Man p-(1→2)- d-Man p 6 and α- d-Man p-(1→3)- d-Man p 7, were synthesised from mannose using the reverse hydrolysis activity of the…”
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Purification of α-galactosidase from Aspergillus niger for application in the synthesis of complex oligosaccharides
Published in Journal of molecular catalysis. B, Enzymatic (01-01-2000)“…An α-galactosidase capable of α-galactosyl transfer on to a 4-position of sugar acceptors was isolated from Aspergillus niger and purified to homogeneity. The…”
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Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis
Published in Current opinion in chemical biology (01-02-1998)“…Remarkable advances in glycobiology in recent years have stimulated a resurgence of interest in carbohydrate chemistry. The challenge of producing the complex…”
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Glycosidases—a great synthetic tool
Published in Journal of molecular catalysis. B, Enzymatic (05-05-1999)“…Glycosidases were used to prepare oligosaccharide structures of physiological and medicinal relevance. The study included an extensive screening of crude…”
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Microbial β- N-acetylhexosaminidases and their biotechnological applications
Published in Enzyme and Microbial Technology (15-07-1999)“…Oligosaccharides containing a β- N-acetylglucosamine moiety are useful building blocks for the synthesis of complex substances with important functions in…”
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Book Review Journal Article -
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Pyruvate Decarboxylase: A Molecular Modeling Study of Pyruvate Decarboxylation and Acyloin Formation
Published in Journal of the American Chemical Society (28-02-1996)“…Using crystal structure data for the pyruvate decarboxylase from Saccharomyces uvarum (which is nearly identical with the enzyme from Saccharomyces…”
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Solvent effect on enzyme-catalyzed synthesis of β- d-glucosides using the reverse hydrolysis method: Application to the preparative-scale synthesis of 2-hydroxybenzyl and octyl β- d-glucopyranosides
Published in Enzyme and microbial technology (01-06-1997)“…Almond β- d-glucosidase was used to catalyze alkyl-β- d-glucoside synthesis by reacting glucose and the alcohol in organic media. The influence of five…”
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Glycosidase-catalysed synthesis of glycosides by an improved procedure for reverse hydrolysis: application to the chemoenzymatic synthesis of galactopyranosyl-(1→4)- O-α-galactopyranoside derivatives
Published in Tetrahedron: asymmetry (01-07-1996)“…β-Galactosidase from Aspergillus oryzae, α-galactosidase from Aspergillus niger, β-mannosidase from Helix pomatia and β-glucosidase from almond were used to…”
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Carbohydrate-protein interactions at interfaces: synthesis of thiolactosyl glycolipids and design of a working model for surface plasmon resonance
Published in Organic & biomolecular chemistry (21-03-2003)“…Thiolactosyl lipids designed for carbohydrate-protein binding studies have been synthesised. One representative was selected for binding studies with a plant…”
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Cn microspheres as surrogate membranes in glycosidase-catalysed hydrolysis of glycolipids
Published in Chemical communications (Cambridge, England) (21-01-2004)“…Glycosidase catalysed hydrolysis of glycolipids non-covalently attached to C(n) microspheres proceeds to completion for appropriate glycolipid-microsphere…”
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Chemoenzymatic synthesis of ethyl 1-thio-(β-D-galactopyranosyl)- O- β-D-glycopyranosyl disaccharides using the β-galactosidase from Bacillus circulans
Published in Tetrahedron: asymmetry (01-03-1996)“…Different ethyl 1-thio-β-D-disaccharides have been synthesised by transgalactosylation using the β-galactosidase from Bacillus circulans as biocatalyst. This…”
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Glycosidase-catalysed oligosaccharide synthesis: preparation of N-acetylchitooligosaccharides using the β- N-acetylhexosaminidase of Aspergillus oryzae
Published in Carbohydrate research (27-12-1995)“…The β- N-acetylhexosaminidase of Aspergillus oryzae catalyses the formation of 2-acetamido-4- O-(2-acetamido-2-deoxy-…”
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Glycosidase-catalysed oligosaccharide synthesis: Preparation of the N-acetylchitooligosaccharidespenta- N-acetylchitopentaose and hexa- N-acetylchitohexaose using the β- N-acetylhexosaminidase of Aspergillus oryzae
Published in Tetrahedron: asymmetry (1995)“…Using a crude β- N-acetylhexosaminidase from Aspergillus oryzae both tri- N-acetylchitotriose (GlcNAc) 3 ( 1, n=1) and tetra- N-acetylchitotetraose (GlcNAc) 4…”
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A Practical Protocol for the Reduction of Disulfide Bonds in Proteins Prior to Analysis by Mass Spectrometry
Published in European journal of mass spectrometry (Chichester, England) (01-02-2001)“…Aquick, simple applicable protocol for the reduction of protein disulfide bonds for the purposes of mass spectrometry has been established. The method utilises…”
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Trisaccharide synthesis by glycosyl transfer from p-nitrophenyl beta-D-N-acetylgalactosaminide on to disaccharide acceptors catalysed by the beta-N-acetylhexosaminidase from Aspergillus oryzae
Published in Carbohydrate research (01-12-1997)“…The beta-N-acetylhexosaminidase from Aspergillus oryzae catalysed the transfer of beta-D-N-acetylgalactosaminyl residues from p-nitrophenyl…”
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Trisaccharide synthesis by glycosyl transfer from p-nitrophenyl [formula omitted] on to disaccharide acceptors catalysed by the β- N-acetylhexosaminidase from Aspergillus oryzae
Published in Carbohydrate research (1997)“…The β- N-acetylhexosaminidase from Aspergillus oryzae catalysed the transfer of β- d-N- acetylgalactosaminyl residues from p-nitrophenyl β- d-N-…”
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Syntheses of pseudo-α-L-fucopyranose and pseudo-6-deoxy-β-D-altropyranose from ((5 S,6 R)-5,6-dihydroxy-1,3-cyclohexadienyl)methanenitrile
Published in Tetrahedron: asymmetry (01-08-1996)“…Pseudo-α-L-fucopyranose 1 and pseudo-6-deoxy-β-D-altropyranose 2 have been synthesised from ((5 S,6 R)-5,6-dihydroxy-1,3-cyclohexadienyl)methanenitrile 3…”
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Enzymatic synthesis of 2-acetamido-4- O-(2-acetamido-2-deoxy-β-d-galactopyranosyl)-2-deoxy-d-glucopyranose and 2-acetamido-6- O-(2-acetamido-2-deoxy-β-d-galactopyranosyl)-2-deoxy-d-glucopyranose catalysed by the β- N-acetylhexosaminidase from Aspergillus oryzae
Published in Carbohydrate research (27-12-1995)Get full text
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