Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny

The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequenc...

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Bibliographic Details
Published in:	Insect biochemistry and molecular biology Vol. 37; no. 12; pp. 1359 - 1365
Main Authors:	Crone, E.J., Zera, A.J., Anand, A., Oakeshott, J.G., Sutherland, T.D., Russell, R.J., Harshman, L.G., Hoffmann, F.G., Claudianos, C.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-12-2007
Subjects:	Amino Acid Sequence amino acid sequences Animals Base Sequence Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - isolation & purification Carboxylic Ester Hydrolases - metabolism Cloning, Molecular complementary DNA enzyme activity Fat Body - enzymology Gastrointestinal Tract - enzymology Gene Library genetic polymorphism Gryllidae Gryllidae - chemistry Gryllidae - enzymology Gryllidae - genetics Gryllus Gryllus assimilis Hemimetabolous insect Hemolymph - enzymology Indel polymorphism Introns Isoenzymes - genetics Isoenzymes - isolation & purification Isoenzymes - metabolism juvenile hormone esterase Juvenile hormone esterase gene Lepidoptera line differences molecular cloning Molecular Probe Techniques Molecular Sequence Data nucleotide sequences phenology Phylogeny Selection, Genetic Sequence Analysis, DNA sequence homology Structure-Activity Relationship structure-activity relationships Indel polymorphism Hemimetabolous insect Juvenile hormone esterase gene Phylogeny Gryllus assimilis
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Summary:	The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from G. assimilis. Partial genomic sequence encoding the Jhe contains two large (1330 and 2918 bp) introns. No coding DNA sequence variation was observed over a 1293 bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19 bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F 2 progeny of crosses between them. Phylogenetic analyses localised the G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.
Bibliography:	http://dx.doi.org/10.1016/j.ibmb.2007.08.005 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0965-1748 1879-0240
DOI:	10.1016/j.ibmb.2007.08.005